公开(公告)号：US06710870B1

公开(公告)日：2004-03-23

申请号：US09601484

申请日：2000-08-03

申请人： Gerd Marowsky ,  Dieter Neuschaefer ,  Michael Pawlak

发明人： Gerd Marowsky ,  Dieter Neuschaefer ,  Michael Pawlak

IPC分类号： G01J330

CPC分类号： G01N21/7703 ,  G01N21/6452 ,  G01N21/69 ,  G01N21/76

摘要： A method exites and determines a luminescence in an analyte sample which is located in contact with the waveguiding layer of an optical layer waveguide. The luminescence is generated by non-evanescent excitation in the volume of the analyte sample. Luminescence radiation generated in the immediate proximity of the surface of the waveguiding layer is conducted to a measuring device and determined after penetrating the waveguiding layer.

摘要翻译： 一种方法显示和确定位于与光学层波导的波导层接触的分析物样品中的发光。 通过在分析物样品的体积中的非消逝激发产生发光。 在波导层的表面附近产生的发光辐射被传导到测量装置，并在穿透波导层之后确定。

公开(公告)号：US20050117161A1

公开(公告)日：2005-06-02

申请号：US10994028

申请日：2004-11-08

申请人： Wolfgang Budach ,  Dieter Neuschaefer

发明人： Wolfgang Budach ,  Dieter Neuschaefer

IPC分类号： G01N21/55 ,  G01B9/02

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2 is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括折射率（n <1> 1）的基底（30）和折射率（n <2）的薄的，光学透明的层（32） >），（n 2）大于（n 1 1/2），平台包括周期性凹槽（31），（31）的形式的一个或多个波纹结构， 这些凹槽定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件，凹槽的形状，尺寸和取向方式使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致 透射光束和入射光的异常高反射从而在该感测区域或每个感测区域的表面处产生增强的消逝场，该场的谐振条件下的幅度比现有技术领域大约100倍 平台，使得从样品o产生的发光强度 平台也增加了100倍。还公开了一种结合平台的装置和使用该平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US06870630B2

公开(公告)日：2005-03-22

申请号：US10684070

申请日：2003-10-09

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01N21/55 ,  G09B9/02

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括在衬底上的折射率（n1）的衬底（30）和折射率（n2）的薄的光透明层（32）（n2）大于（n1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的异常高反射从而产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，包括 平台和使用平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US20060216204A1

公开(公告)日：2006-09-28

申请号：US11350665

申请日：2006-02-08

申请人： Wolfgang Ernst Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Budach ,  Dieter Neuschaefer

IPC分类号： G01N21/64 ,  G01B9/02

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括折射率（n <1> 1）的基底（30）和折射率（n <2）的薄的，光学透明的层（32） >）在（n 2/2）上大于（n <1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的异常高反射从而产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，包括 平台和使用平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US06771376B2

公开(公告)日：2004-08-03

申请号：US10043629

申请日：2002-01-10

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01B902

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括在衬底上的折射率（n1）的衬底（30）和折射率（n2）的薄的，光学透明的层（32）（n2）大于（n1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的异常高反射从而产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，包括 平台和使用平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US07820106B2

公开(公告)日：2010-10-26

申请号：US11350665

申请日：2006-02-08

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01N21/00

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括在衬底上的折射率（n1）的衬底（30）和折射率（n2）的薄的光透明层（32）（n2）大于（n1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的异常高反射从而产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，包括 平台和使用平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US07064844B2

公开(公告)日：2006-06-20

申请号：US10994028

申请日：2004-11-08

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01B9/02 ,  G01N21/64 ,  G01N21/66

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2 is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括折射率（n <1> 1）的基底（30）和折射率（n <2）的薄的，光学透明的层（32） >），（n 2）大于（n 1 1/2），平台包括周期性凹槽（31），（31）的形式的一个或多个波纹结构， 这些凹槽定义了一个或多个感测区域，每个感应区域用于一个或多个捕获元件，凹槽的形状，尺寸和取向方式使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致 透射光束和入射光的异常高反射从而在该感测区域或每个感测区域的表面处产生增强的消逝场，该场的谐振条件下的幅度比现有技术领域大约100倍 平台，使得从样品o产生的发光强度 平台也增加了100倍。还公开了一种结合平台的装置和使用该平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US06867869B2

公开(公告)日：2005-03-15

申请号：US10684071

申请日：2003-10-09

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01N21/55 ,  G01B9/02

CPC分类号： G01N21/648 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

摘要翻译： 用于样品分析的传感器平台包括在衬底上的折射率（n1）的衬底（30）和折射率（n2）的薄的光透明层（32）（n2）大于（n1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，它被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的异常高反射从而产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，包括 平台和使用平台的方法。 通过使用具有引起TM激发和/或从基板侧照射平台的线性分量的光来检测振幅的进一步增加。

公开(公告)号：US06707561B1

公开(公告)日：2004-03-16

申请号：US09609846

申请日：2000-07-05

申请人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

发明人： Wolfgang Ernst Gustav Budach ,  Dieter Neuschaefer

IPC分类号： G01B902

CPC分类号： G01N21/648 ,  B82Y30/00 ,  G01N21/7743

摘要： A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n1) and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, (n2) is greater than (n1). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an anormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed is an apparatus incorporating the platform and a method of using the platform.

摘要翻译： 用于样品分析的传感器平台包括在衬底上的折射率（n1）的衬底（30）和折射率（n2）的薄的光透明层（32）（n2）大于（n1）。 平台包含周期性凹槽（31），（33）形式的一个或多个波纹结构，其定义了一个或多个感测区域，每个感测区域用于一个或多个捕获元件。 凹槽是如此成型，尺寸和取向，使得当相干光入射到平台上时，其被衍射成单独的光束或衍射级，导致透射光束的减少，并且入射光的正常高反射从而在...处产生增强的渐逝场 或每个感测区域的表面。 谐振条件下该场的振幅比现有技术的平台大约大约100倍，使得从平台上的样本产生的发光强度也增加了100倍。还公开了一种装置，其结合 平台和使用平台的方法。