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公开(公告)号：US06710870B1
公开(公告)日：2004-03-23
申请号：US09601484
申请日：2000-08-03
IPC分类号： G01J330
CPC分类号： G01N21/7703 , G01N21/6452 , G01N21/69 , G01N21/76
摘要： A method exites and determines a luminescence in an analyte sample which is located in contact with the waveguiding layer of an optical layer waveguide. The luminescence is generated by non-evanescent excitation in the volume of the analyte sample. Luminescence radiation generated in the immediate proximity of the surface of the waveguiding layer is conducted to a measuring device and determined after penetrating the waveguiding layer.
摘要翻译： 一种方法显示和确定位于与光学层波导的波导层接触的分析物样品中的发光。 通过在分析物样品的体积中的非消逝激发产生发光。 在波导层的表面附近产生的发光辐射被传导到测量装置，并在穿透波导层之后确定。
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2.发明申请Grating waveguide structure for reinforcing an excitation field and use thereof 审中-公开用于加强激励场的光栅波导结构及其用途公开(公告)号：US20090224173A1
公开(公告)日：2009-09-10
申请号：US12318568
申请日：2008-12-31
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/774 , G01N2021/6419 , G01N2021/7786 , G02B6/124
摘要： The invention relates to a variable embodiment of a grating waveguide structure, based on a planar thin-film waveguide with a first optically transparent layer (a) on a second optically transparent layer (b) having a lower refractive index than layer (a), and a grating structure (c) modulated in layer (a), wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 100 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light. The invention also relates to an optical system with an excitation light source and an embodiment of a grating waveguide structure according to the invention, and to a method for enhancing an excitation light intensity, and to the use thereof in bioanalytical detection processes, in non-linear optics or in telecommunications or communications industry.
摘要翻译： 本发明涉及一种基于具有比层（a）折射率低的第二光学透明层（b）上具有第一光学透明层（a）的平面薄膜波导的光栅波导结构的可变实施例， 以及在层（a）中调制的光栅结构（c），其中以共耦合角度照射到层（a）中的激发光的强度在层（a）上至少增加100倍，并且在层 （a），至少在光栅结构（c）的区域中，与衬底表面上的所述激发光的强度相比较，而不会激发激发光的耦合。 本发明还涉及具有激发光源和根据本发明的光栅波导结构的实施例的光学系统，以及用于增强激发光强度的方法及其在生物分析检测过程中的用途， 线性光学或电信或通信行业。
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3.发明申请公开(公告)号：US20100130370A1
公开(公告)日：2010-05-27
申请号：US12695553
申请日：2010-01-28
申请人： Eveline Schurmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Mtthias Kresbach , Michael Pawlak , Nania Graciela Scharer-Hernandez , Eginhard Schick
发明人： Eveline Schurmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Mtthias Kresbach , Michael Pawlak , Nania Graciela Scharer-Hernandez , Eginhard Schick
CPC分类号： B01L3/5025 , G01N21/05 , G01N21/253 , G01N2021/0346
摘要： A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents. An arrangement of one or more sample compartments includes a base plate and a body combined with the base plate in such a way that one or more recesses for generation of one or more flow cells fluidically sealed against one another, each with at least one inlet and one outlet, are formed between the base plate and the body, wherein at least one outlet of each flow cell is joined with a reservoir fluidically connected with the flow cell, the reservoir being operable to receive liquid exiting the flow cell, besides methods for its manufacturing and their use.
摘要翻译： 提供了流动池的一维或二维布置，作为样品室阵列的一部分，每个样品室的至少一个入口和出口由基板和主体形成，具有空间凹陷的布置 对应于样品室的（几何）布置，与基板结合。 该装置允许从样品室供应或从样品室中取出，样品室可以大量排列在小的基底区域上，甚至是非常少量的样品或试剂。 一个或多个样品室的布置包括基板和与基板组合的主体，使得一个或多个凹槽用于产生流体密封的一个或多个流动池，每个具有至少一个入口和 一个出口形成在基板和主体之间，其中每个流动池的至少一个出口与与流动池流体连接的储存器连接，储存器可操作以接收离开流动池的液体，除了其用于其的方法 制造及其使用。
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4.发明申请Analytical platform and detection method with the analytes to be determined in a sample as immobilized specific binding partners 审中-公开分析平台和检测方法与样品中待测定的分析物作为固定化的特异性结合配偶体公开(公告)号：US20050059014A1
公开(公告)日：2005-03-17
申请号：US10653386
申请日：2003-09-03
申请人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
发明人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
IPC分类号： G01N33/566 , C12M1/00 , C12M1/34 , C12Q1/68 , G01N21/76 , G01N33/53 , G01N33/543 , G01N33/553
CPC分类号： G01N33/54353 , G01N33/54373
摘要： The present invention is related to an analytical platform and a method performed therewith for the analysis of a multitude of “nature-identical” samples for analytes which are contained therein and are of biological relevance as binding partners in specific binding reactions, wherein said samples or dilutions of said samples of the same relative molecular composition as the original samples, with the analytes to be determined contained therein, as a first plurality of specific binding partners, are deposited in discrete measurement areas in at least one one- or two-dimensional array of measurement areas on an evanescent field sensor platform as a solid support without a change in the relative molecular composition, compared with the original relative molecular composition of the sample, one or more tracer compounds as a second plurality of specific binding partners, for the specific determination of analytes out of the first plurality of specific binding partners contained in the samples, are brought into contact with the samples deposited in said discrete measurement areas in a single step or multiple steps of a specific binding reaction, changes in opto-electronic signals, resulting from the binding of tracer compounds to analytes contained in the samples in discrete measurement areas or to specific binding partners bound to the analytes contained in the measurement areas in the evanescent field of the evanescent field sensor platform are measured laterally resolved, and the presence of the analytes to be specifically detected is determined qualitatively and/or quantitatively from the relative amount of the changes in said opto-electronic signals from the corresponding measurement areas.
摘要翻译： 本发明涉及一种分析平台及其分析方法，用于分析大量与分析物相似的“自然相同”的样品，其中所含的分析物样品在其中包含并具有生物相关性，作为特异性结合反应中的结合配偶体，其中所述样品或 与原始样品相同的相对分子组成的所述样品与其中包含的待测定的分析物作为第一多个特异性结合配偶体的稀释沉积在至少一个一维或二维阵列的离散测量区域中 相对于样品的原始相对分子组成，作为第二多个特异性结合配偶体的一个或多个示踪剂化合物，作为固体支持物的ev逝场传感器平台上的测量区域作为固体支持物而没有相对分子组成的变化 确定包含在第一个多个具体约束伙伴中的分析物 在与单独的步骤或特定结合反应的多个步骤中，沉积在所述离散测量区域中的样品与示踪剂化合物与样品中包含的分析物的离散性的光电子信号的变化相接触 测量区域或与消逝场传感器平台的消逝场中测量区域中包含的分析物结合的特异性结合配体进行横向分辨测定，并且特异性检测的分析物的存在是从定性和/或定量的 相应测量区域的所述光电信号的相对变化量。
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5.发明申请Bioanalytical reagent, method for production thereof, sensor platforms and detection methods based on use of said bioanalytical reagent 审中-公开生物分析试剂，其生产方法，传感器平台和基于所述生物分析试剂的检测方法公开(公告)号：US20050019836A1
公开(公告)日：2005-01-27
申请号：US10433823
申请日：2001-12-04
申请人： Horst Vogel , Horst Pick , Axel Preuss , Ana-Paula Tairi , Evelyne Schmid Osborne , Michael Pawlak
发明人： Horst Vogel , Horst Pick , Axel Preuss , Ana-Paula Tairi , Evelyne Schmid Osborne , Michael Pawlak
IPC分类号： G01N33/554 , G01N33/53 , C07K14/705 , C07K16/28 , G01N33/567
CPC分类号： G01N33/554
摘要： The invention relates to various embodiments of a bioanalytical reagent with at least one vesicle, generated from a living cell, comprising at least one receptor, characterized in that a mechanism of signal transduction triggered by said receptor in the cell used for vesicle generation is preserved in said vesicle, as a component of the bioanalytical reagent. The invention further relates to methods for production of the bioanalytical reagent according to the invention, to bioanalytical detection methods based on the application of said reagent, and to the use of said detection method and of the bioanalytical reagent.
摘要翻译： 本发明涉及生物分析试剂的各种实施方案，其具有由活细胞产生的至少一种囊泡，其包含至少一种受体，其特征在于，用于产生囊泡的细胞中由所述受体触发的信号转导机制被保留在 所述囊泡作为生物分析试剂的组分。 本发明还涉及根据本发明的生物分析试剂的生产方法，基于所述试剂的应用的生物分析检测方法，以及所述检测方法和生物分析试剂的使用。
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6.发明授权公开(公告)号：US07678565B2
公开(公告)日：2010-03-16
申请号：US12071526
申请日：2008-02-21
申请人： Eveline Schürmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Schärer-Hernández , Eginhard Schick
发明人： Eveline Schürmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Schärer-Hernández , Eginhard Schick
CPC分类号： B01L3/5025 , G01N21/05 , G01N21/253 , G01N2021/0346
摘要： A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents. An arrangement of one or more sample compartments includes a base plate and a body combined with the base plate in such a way that one or more recesses for generation of one or more flow cells fluidically sealed against one another, each with at least one inlet and one outlet, are formed between the base plate and the body, wherein at least one outlet of each flow cell is joined with a reservoir fluidically connected with the flow cell, the reservoir being operable to receive liquid exiting the flow cell, besides methods for its manufacturing and their use.
摘要翻译： 提供了流动池的一维或二维布置，作为样品室阵列的一部分，每个样品室的至少一个入口和出口由基板和主体形成，具有空间凹陷的布置 对应于样品室的（几何）布置，与基板结合。 该装置允许从样品室供应或从样品室中取出，样品室可以大量排列在小的基底区域上，甚至是非常少量的样品或试剂。 一个或多个样本室的布置包括基板和与基板结合的主体，使得一个或多个凹槽用于产生流体密封的一个或多个流动池，每个具有至少一个入口和 一个出口形成在基板和主体之间，其中每个流动池的至少一个出口与与流动池流体连接的储存器连接，储存器可操作以接收离开流动池的液体，除了其用于其的方法 制造及其使用。
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7.发明申请Grating waveguide structure for multi-analyte determinations and the use thereof 审中-公开用于多分析物测定的光栅波导结构及其用途公开(公告)号：US20090054263A1
公开(公告)日：2009-02-26
申请号：US12222212
申请日：2008-08-05
CPC分类号： G01N21/7743 , G01N33/54373
摘要： The invention relates to variable embodiments of a grating waveguide structure which enables to determine locally resolved changes of the resonance conditions for the incoupling of an excitation light into the waveguiding layer (a) of a stratified optical waveguide by means of a grating structure (c) modulated in said layer (a) or for outcoupling of a light guided in layer (a). The inventive system comprises arrays of measurement areas produced on the grating waveguide structure having different immobilized biological or biochemical or synthetic recognition elements for simultaneously binding and determining one or more analytes, wherein said excitation light is simultaneously irradiated onto an entire array of measurement areas, and the degree of satisfaction of the resonance condition for the incoupling of light into the layer (a) towards said measurement areas is simultaneously measured. The invention also relates to an optical system comprising at least one excitation light source and at least one locally resolving detector and, optionally, positioning elements for altering the angle of incidence of the excitation light onto the inventive grating waveguide structure. The invention additionally relates to a corresponding measuring method and to the use thereof. Surprisingly, it has been found that the inventive method is well-suited as an imaging detection method with high local resolution and sensitivity.
摘要翻译： 本发明涉及光栅波导结构的可变实施例，其能够通过光栅结构（c）确定激发光进入分层光波导的波导层（a）的谐振条件的局部解析变化， 在所述层（a）中调制或者在层（a）中引导的光的外耦合。 本发明的系统包括在光栅波导结构上产生的具有不同的固定的生物或生物化学或合成识别元件的测量区域的阵列，用于同时结合和确定一种或多种分析物，其中所述激发光同时照射到整个测量区域阵列上，以及 同时测量对于朝向所述测量区域将光耦合到层（a）中的共振条件的满足程度。 本发明还涉及一种光学系统，其包括至少一个激发光源和至少一个局部分辨检测器以及可选地用于将激发光的入射角改变到本发明的光栅波导结构上的定位元件。 本发明还涉及相应的测量方法及其用途。 令人惊讶的是，已经发现本发明的方法非常适合作为具有高的局部分辨率和灵敏度的成像检测方法。
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公开(公告)号：US20080299008A1
公开(公告)日：2008-12-04
申请号：US12071526
申请日：2008-02-21
申请人： Eveline Schurmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Scharer-Hernandez , Eginhard Schick
发明人： Eveline Schurmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Scharer-Hernandez , Eginhard Schick
CPC分类号： B01L3/5025 , G01N21/05 , G01N21/253 , G01N2021/0346
摘要： A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents. An arrangement of one or more sample compartments includes a base plate and a body combined with the base plate in such a way that one or more recesses for generation of one or more flow cells fluidically sealed against one another, each with at least one inlet and one outlet, are formed between the base plate and the body, wherein at least one outlet of each flow cell is joined with a reservoir fluidically connected with the flow cell, the reservoir being operable to receive liquid exiting the flow cell, besides methods for its manufacturing and their use.
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公开(公告)号：US06384912B1
公开(公告)日：2002-05-07
申请号：US09742391
申请日：2000-12-22
申请人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
发明人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
IPC分类号： G01N110
摘要： A device has a planar optical waveguide, including a transparent carrier (40) and a wave-guiding layer (41), wherein the waveguide at least has a diffractive element (42) for coupling excitation radiation into the wave-guiding layer. Located on the wave-guiding layer is a further, tightly sealing layer (43) made from a material which, at least at the support surface, is transparent both to the excitation radiation and to the evanescently excited radiation at least to the penetration depth of the evanescent field, and which, for an analysis sample, has, at least in a partial region of the guided excitation radiation, a cavity (45) which is open to the upper side or a cavity (6) which is closed to the upper side and connected by means of an inlet channel (2) and an outlet channel (3). The depth of the cavity corresponds at least to the penetration depth of the evanescent field, and the diffractive element (42) is fully covered by the material of the layer (43) at least in the coupling-in region of the excitation radiation.
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10.发明授权公开(公告)号：US08053225B2
公开(公告)日：2011-11-08
申请号：US12695553
申请日：2010-01-28
申请人： Eveline Schürmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Schärer-Hernández , Eginhard Schick
发明人： Eveline Schürmann-Mader , Andreas Peter Abel , Martin Andreas Bopp , Gert Ludwig Duveneck , Markus Ehrat , Gerhard Matthias Kresbach , Michael Pawlak , Nania Graciela Schärer-Hernández , Eginhard Schick
CPC分类号： B01L3/5025 , G01N21/05 , G01N21/253 , G01N2021/0346
摘要： A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents. An arrangement of one or more sample compartments includes a base plate and a body combined with the base plate in such a way that one or more recesses for generation of one or more flow cells fluidically sealed against one another, each with at least one inlet and one outlet, are formed between the base plate and the body, wherein at least one outlet of each flow cell is joined with a reservoir fluidically connected with the flow cell, the reservoir being operable to receive liquid exiting the flow cell, besides methods for its manufacturing and their use.
摘要翻译： 提供了流动池的一维或二维布置，作为样品室阵列的一部分，每个样品室的至少一个入口和出口由基板和主体形成，具有空间凹陷的布置 对应于样品室的（几何）布置，与基板结合。 该装置允许从样品室供应或从样品室中取出，样品室可以大量排列在小的基底区域上，甚至是非常少量的样品或试剂。 一个或多个样本室的布置包括基板和与基板结合的主体，使得一个或多个凹槽用于产生流体密封的一个或多个流动池，每个具有至少一个入口和 一个出口形成在基板和主体之间，其中每个流动池的至少一个出口与与流动池流体连接的储存器连接，储存器可操作以接收离开流动池的液体，除了其用于其的方法 制造及其使用。
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