公开(公告)号：US20140030727A1

公开(公告)日：2014-01-30

申请号：US13747306

申请日：2013-01-22

申请人： Gerd Pfeifer ,  Seung-Gi Jin ,  Yong Jiang ,  Runxiang Qiu ,  Qiang Lu

发明人： Gerd Pfeifer ,  Seung-Gi Jin ,  Yong Jiang ,  Runxiang Qiu ,  Qiang Lu

IPC分类号： G01N33/574 ,  G01N33/483

CPC分类号： G01N33/57496 ,  G01N33/4833

摘要： Methods for detecting or diagnosing cancer in a subject are provided herein. Such methods may include, but are not limited to, measuring a test level of 5hmC in a biological sample from the subject; and determining that the subject has a malignant cancer when the test level of 5hmC is lower than that of a control level of 5hmC. Such methods may further include a step of measuring a test level of Ki67 in the biological sample and determining that the subject has a malignant cancer when the test level of Ki67 is higher than that of a control level of Ki67.

摘要翻译： 本文提供了用于检测或诊断受试者中的癌症的方法。 这样的方法可以包括但不限于测量来自受试者的生物样品中的5hmC的测试水平; 并且当5hmC的测试水平低于5hmC的对照水平时，确定受试者具有恶性癌症。 这样的方法还可以包括当Ki67的检测水平高于对照水平的Ki67时，测量生物样品中Ki67的测试水平并确定受试者具有恶性癌症的步骤。

公开(公告)号：US08513401B2

公开(公告)日：2013-08-20

申请号：US12772652

申请日：2010-05-03

申请人： John J. Rossi ,  Daniela Castanotto ,  Gerd Pfeifer ,  Stella Tommasi ,  Kevin V. Morris ,  Daniel H. Kim

发明人： John J. Rossi ,  Daniela Castanotto ,  Gerd Pfeifer ,  Stella Tommasi ,  Kevin V. Morris ,  Daniel H. Kim

IPC分类号： C07H21/04 ,  C07H21/02

CPC分类号： C12N15/111 ,  C12N15/1135 ,  C12N15/63 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

摘要： The present invention relates to transcriptional gene silencing (TGS) in mammalian, including human, cells that is mediated by small interfering RNA (siRNA) molecules. The present invention also relates to a double stranded nucleic acid that directs methylation of histones associated with target genes that produce low copy promoter-specific RNA. It has been found that siRNAs can be used to direct methylation of histones in mammalian, including human, cells.

摘要翻译： 本发明涉及哺乳动物（包括人）由小干扰RNA（siRNA）分子介导的细胞的转录基因沉默（TGS）。 本发明还涉及引导与产生低拷贝启动子特异性RNA的靶基因相关的组蛋白甲基化的双链核酸。 已经发现siRNA可以用于引导哺乳动物（包括人）细胞中组蛋白的甲基化。

公开(公告)号：US20060240460A1

公开(公告)日：2006-10-26

申请号：US11398765

申请日：2006-04-06

申请人： Gerd Pfeifer ,  Tibor Rauch

发明人： Gerd Pfeifer ,  Tibor Rauch

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6813 ,  C12Q2537/164

摘要： The present invention provides new and improved assay for detection of genomic methylated CpG islands. This new method is termed the methylated-CpG island recovery assay (MIRA). In accordance with one embodiment, MIRA comprises the steps of: (a) incubating genomic DNA fragments with a methylated CpG island binding protein in the presence of a binding partner for the binding protein to produce bound DNA containing methylated CpG islands, (b) isolating the bound DNA, and (c) detecting CpG island methylation by gene-specific amplification reactions. In accordance with a preferred embodiment, MIRA comprises the steps of: (a) incubating sonicated genomic DNA with a matrix containing a fusion protein of glutathione S-transferase (GST) and MBD2b (GST-MBD2b) in the presence of MBD3L1 to produce bound DNA containing methylated CpG islands, (b) eluting bound DNA from the matrix, and (c) detecting CpG island methylation by gene-specific amplification reactions.

摘要翻译： 本发明提供用于检测基因组甲基化CpG岛的新的和改进的测定。 这种新方法被称为甲基化CpG岛回收测定（MIRA）。 根据一个实施方案，MIRA包括以下步骤：（a）在结合蛋白的结合配偶体存在下，将基因组DNA片段与甲基化CpG岛结合蛋白质孵育以产生含有甲基化CpG岛的结合DNA，（b）分离 结合DNA，和（c）通过基因特异性扩增反应检测CpG岛甲基化。 根据优选实施方案，MIRA包括以下步骤：（a）在MBD3L1存在下，将超声处理的基因组DNA与含有谷胱甘肽S-转移酶（GST）和MBD2b（GST-MBD2b）的融合蛋白的基质一起温育以产生结合 含有甲基化CpG岛的DNA，（b）从基质中洗脱结合的DNA，和（c）通过基因特异性扩增反应检测CpG岛甲基化。