公开(公告)号：US06609015B2

公开(公告)日：2003-08-19

申请号：US09912127

申请日：2001-07-24

申请人： Gerhardus Wilhelmus Lucassen ,  Gerwin Jan Puppels ,  Peter Jacobus Caspers ,  Marjolein Van Der Voort ,  Egbert Lenderink ,  Martinus Bernardus Van Der Mark ,  Robert Frans Maria Hendriks ,  Julius Simon Cohen

发明人： Gerhardus Wilhelmus Lucassen ,  Gerwin Jan Puppels ,  Peter Jacobus Caspers ,  Marjolein Van Der Voort ,  Egbert Lenderink ,  Martinus Bernardus Van Der Mark ,  Robert Frans Maria Hendriks ,  Julius Simon Cohen

IPC分类号： A61B500

CPC分类号： G01N21/65 ,  A61B5/0068 ,  A61B5/0075 ,  A61B5/489 ,  A61B5/6824 ,  A61B5/7207 ,  A61B2562/0242 ,  G01N2021/656 ,  G02B21/0024 ,  G02B21/008

摘要： An analysis apparatus including a spectroscopic analysis apparatus comprises an excitation system and a monitoring system. The excitation system emits an excitation beam to excite a target region during an excitation period. The monitoring system emits a monitoring beam to image the target region during a monitoring period. The excitation period and the monitoring period substantially overlap. Hence the target region is imaged together with the excitation, and an image is formed displaying both the target region and the excitation area. On the basis of this image, the excitation beam can be very accurately aimed at the target region.

公开(公告)号：US08368884B2

公开(公告)日：2013-02-05

申请号：US11911557

申请日：2006-04-19

申请人： Gerhardus Wilhelmus Lucassen ,  Rufus Driessen ,  Kristiane Anne Schmidt ,  Gerwin Jan Puppels ,  Peter Jacobus Caspers

发明人： Gerhardus Wilhelmus Lucassen ,  Rufus Driessen ,  Kristiane Anne Schmidt ,  Gerwin Jan Puppels ,  Peter Jacobus Caspers

IPC分类号： G01J3/30 ,  A61B5/1455

CPC分类号： A61B5/14532 ,  A61B5/489

摘要： A spectroscopic apparatus for determining a concentration and/or spatial gradient of an analyte of a bodily fluid that provides determination of a position of a capillary vessel within a biological sample in order to focus spectroscopic excitation radiation to a volume that is in close proximity to the capillary vessel but does not overlap with the capillary vessel. The provided apparatus exploits the permeability of the vessel wall with respect to an analyte that is subject to analyte concentration determination. By means of biological transport processes, the concentration of an analyte of a bodily fluid located in the capillary vessel influences the concentration in the surrounding of the capillary vessel. Spectroscopic analysis of a volume outside the capillary vessel can therefore serve for a precise and reliable analyte concentration determination inside the capillary vessel.

摘要翻译： 一种用于确定体液分析物的浓度和/或空间梯度的分光装置，其提供对生物样品内毛细血管位置的确定，以便将分光激发辐射聚焦到接近于 毛细血管，但不与毛细血管重叠。 所提供的装置相对于经受分析物浓度测定的分析物利用血管壁的渗透性。 通过生物传输过程，位于毛细管中的体液分析物的浓度影响毛细血管周围的浓度。 因此，毛细血管外部的体积的光谱分析可用于在毛细血管内进行精确和可靠的分析物浓度测定。

公开(公告)号：US07656522B2

公开(公告)日：2010-02-02

申请号：US10506056

申请日：2002-02-28

申请人： Gerwin Jan Puppels ,  Peter Jacobus Caspers ,  Gerhardus Wilhelmus Lucassen

发明人： Gerwin Jan Puppels ,  Peter Jacobus Caspers ,  Gerhardus Wilhelmus Lucassen

IPC分类号： G01J3/44

CPC分类号： A61B5/14539 ,  A61B5/0059 ,  A61B5/0064 ,  A61B5/441 ,  A61B2562/0242 ,  G01N21/65

摘要： A Raman spectrum is measured inside animal tissue, such us human skin tissue, at a selected depth from a surface the tissue. A pH value is computed using a function that assigns a pH value as a function of the measured Raman spectrum. The computation may involve computing a number representing a ratio of concentrations of a protonated and a deprotonated version of a chemical substance from the Raman spectrum and generating pH information on the basis of said number. The chemical substance is for example a form of Urocanic acid (UCA). UV exposure is measured from the weight of the spectrum of cis-UCA.

摘要翻译： 在动物组织内测量拉曼光谱，例如人类皮肤组织，从表面的选定深度组织。 使用赋予pH值作为测量的拉曼光谱的函数的函数来计算pH值。 该计算可以涉及计算表示来自拉曼光谱的化学物质的质子化和去质子化版本的浓度比的数量，并且基于所述数量产生pH信息。 化学物质例如是一种形式的尿布酸（UCA）。 紫外线照射是从顺式UCA光谱的重量测定的。

公开(公告)号：US20120116232A1

公开(公告)日：2012-05-10

申请号：US13249500

申请日：2011-09-30

申请人： Peter Jacobus Caspers ,  Grainne O'Regan ,  Johanna de Sterke ,  Gerwin Jan Puppels

发明人： Peter Jacobus Caspers ,  Grainne O'Regan ,  Johanna de Sterke ,  Gerwin Jan Puppels

IPC分类号： A61B6/00

CPC分类号： A61B5/445 ,  A61B5/0075 ,  A61B5/7264 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N21/65

摘要： The present invention relates to a method of using Raman spectra to identify unknown mutations in a gene, more specifically the filaggrin gene. Specifically, the present invention relates to a method to determine if a person has a homozygous or compound heterozygous mutation in the filaggrin-gene comprising measurement of the presence of tyrosine in the skin. Preferably said measurements are performed by Rama spectrography and on the tsiie of the skin, most preferably on the palm of the hand. Further, when these measurements are taken together with measurements of the NMF content of the skin it, the present invention relates to a method of classifying atopic dermatitis.

摘要翻译： 本发明涉及使用拉曼光谱来鉴定基因中未知突变的方法，更具体地说是丝聚蛋白基因。 具体地，本发明涉及一种确定人是否在丝聚蛋白基因中具有纯合或复合杂合突变的方法，包括测量皮肤中酪氨酸的存在。 优选地，所述测量由拉玛光谱法和皮肤上的皮肤进行，最优选在手掌上进行。 此外，当这些测量与皮肤的NMF含量的测量一起使用时，本发明涉及对特应性皮炎进行分类的方法。

公开(公告)号：US20100322860A1

公开(公告)日：2010-12-23

申请号：US12666311

申请日：2008-06-25

申请人： Peter Jacobus Caspers ,  Hok Bing Thio ,  Gerwin Jan Puppels ,  Patrick M.J.H. Kemperman ,  Hendrik Arent Martino Neumann

发明人： Peter Jacobus Caspers ,  Hok Bing Thio ,  Gerwin Jan Puppels ,  Patrick M.J.H. Kemperman ,  Hendrik Arent Martino Neumann

IPC分类号： A61K49/00 ,  G01N33/483 ,  A61P43/00

CPC分类号： A61B5/0059 ,  A61B5/411 ,  A61B5/445 ,  A61B5/4869 ,  A61B2562/0242

摘要： The invention is directed to a method for non-invasive determination of the potential presence of one or more loss-of-function mutation(s) in the gene encoding for filaggrin.The method of the invention comprises (i) obtaining a vibrational spectrum from the stratum corneum of the individual; (ii) determining the local natural moisturising factor content from the vibrational spectrum; (iii) optionally repeating steps (i) and (ii); and (iv) comparing the local natural moisturising factor content of the individual to a reference value, wherein said stratum corneum is stratum corneum of a location of the body of said individual at which said one or more loss-of-function mutation(s) in the gene encoding for filaggrin has a stronger influence on the natural moisturising factor concentration than other factors influencing the natural moisturising factor concentration.

摘要翻译： 本发明涉及用于非侵入性确定编码丝聚蛋白的基因中一种或多种功能丧失功能突变的潜在存在的方法。 本发明的方法包括（i）从个体的角质层获得振动光谱; （ii）从振动光谱确定局部天然保湿因子含量; （iii）任选地重复步骤（i）和（ii）; 和（iv）将个体的局部天然保湿因子含量与参考值进行比较，其中所述角质层是所述个体的身体位置的角质层，其中所述一种或多种功能丧失突变 在编码丝聚蛋白的基因中，与其他影响天然保湿因子浓度的因素相比，对天然保湿因子浓度的影响更大。