公开(公告)号：US06214593B1

公开(公告)日：2001-04-10

申请号：US09194595

申请日：1998-11-27

申请人： Giovanni Carturan ,  Maurizio Muraca ,  Renzo Dal Monte

发明人： Giovanni Carturan ,  Maurizio Muraca ,  Renzo Dal Monte

IPC分类号： C12N1114

CPC分类号： A61K9/0024 ,  C12N5/0012 ,  C12N11/04 ,  C12N2533/10

摘要： A suspension of animal cells is incubated with supports to adhere the cells to the supports. Preferably, the supports have pores that provide pore volume, and the cells are grown during incubation until most of the available pore volume is filled with cells. An encapsulating layer is then formed around the supported cells by exposing the cells on the supports to a reactive gas composed of a carrier gas such as sterile air saturated with an inorganic alkoxide followed by treatment with steam to hydrolyze residual alkoxide groups. The encapsulated cells are stored by immersion in culture media. The cells may be in the form of cell aggregates, and the supports can be sterilized. The supports and encapsulating layer can have pores of a size that permit free exchange nutrients and metabolic products, and excludes the cells from contacting antibodies or immune cells when implanted. The encapsulated cells can used in an extracorporeal device or implanted directly.

摘要翻译： 将动物细胞的悬浮液与载体一起孵育以将细胞粘附到载体上。 优选地，支持体具有提供孔体积的孔，并且细胞在孵育期间生长直到大部分可用孔体积充满细胞。 然后通过将载体上的细胞暴露于由诸如无机醇盐饱和的无菌空气的载气组成的反应性气体，然后用蒸汽处理以水解残余的醇盐基团，在支撑的细胞周围形成包封层。 将包封的细胞通过浸入培养基中储存。 细胞可以是细胞聚集体的形式，并且支持物可被灭菌。 载体和包封层可以具有允许自由交换营养物和代谢产物的尺寸的孔，并且在植入时排除细胞接触抗体或免疫细胞。 封装的细胞可用于体外器械或直接植入。

公开(公告)号：US5998162A

公开(公告)日：1999-12-07

申请号：US952104

申请日：1997-11-17

申请人： Elsa Mariella Cappelletti ,  Giovanni Carturan ,  Anna Piovan

发明人： Elsa Mariella Cappelletti ,  Giovanni Carturan ,  Anna Piovan

IPC分类号： C12M3/00 ,  C12N5/04 ,  C12N11/04 ,  C12N11/14 ,  C12P1/00 ,  C12N5/00

CPC分类号： C12N11/14 ,  C12N11/04

摘要： Secondary metabolites of viable plant cells are produced with the cells immobilized in a porous inorganic support. Immobilization includes the steps of: (a) preparing a support comprising a substantially uniform and porous matrix of inorganic material having a tensile strength of at least 500 MPa; (b) introducing a culture of viable plant cells into the pores of said matrix; (c) entrapping the plant cells by coating the matrix with a sol or colloidal suspension not interfering with the cell viability; and (d) immobilizing the entrapped cells within the matrix with a reactive gas including a carrier gas saturated with volatile SiO.sub.2 or organic modified SiO.sub.2 precursors. The tensile strength may be provided by impregnating the matrix with a gelling solution of SiO.sub.2 precursor for increasing stiffness of the matrix. The matrix may be a SiO.sub.2 or inorganic oxide matrices, in which the weight ratio between cell load and inorganic matrix ranges between 1.times.10.sup.-4 and 1.times.10.sup.-2. The immobilized cells are not released in solution over a period of 6 months and maintain their viability while producing secondary metabolites.

摘要翻译： PCT No.PCT / IT95 / 00083 Sec。 371日期：1997年11月17日 102（e）日期1997年11月17日PCT提交1995年5月18日PCT公布。 WO96 / 36703 PCT出版物 日期1996年11月21日生物植物细胞的第二代代谢物是用固定在多孔无机载体中的细胞产生的。 固定包括以下步骤：（a）制备包含抗拉强度至少为500MPa的基本均匀且多孔的无机材料基质的载体; （b）将活的植物细胞的培养物引入所述基质的孔中; （c）通过用不干扰细胞活力的溶胶或胶体悬浮液涂覆基质来捕获植物细胞; 和（d）用含有挥发性SiO 2或有机改性SiO 2前体饱和的载气的活性气体将夹带的细胞固定在基质内。 拉伸强度可以通过用SiO 2前体的胶凝溶液浸渍基质来提供，以增加基体的刚度。 基质可以是SiO 2或无机氧化物基质，其中电池负载和无机基质之间的重量比在1×10 -4和1×10 -2之间。 固定化细胞在溶液中不会在6个月的时间内释放，并保持其生存力同时产生次生代谢物。

公开(公告)号：US5169809A

公开(公告)日：1992-12-08

申请号：US470167

申请日：1990-01-25

申请人： Umberto Brenna ,  Giovanni Carturan ,  Gianmarco Del Felice ,  Mirto Mozzon

发明人： Umberto Brenna ,  Giovanni Carturan ,  Gianmarco Del Felice ,  Mirto Mozzon

IPC分类号： C07C31/28 ,  C04B35/622 ,  C07C49/14 ,  D01D5/06 ,  D01F9/08

CPC分类号： C04B35/6224 ,  C04B35/6225 ,  D01F9/08

摘要： The present invention relates to ZrO.sub.2 - and SiO.sub.2 -based ceramic fibers in which the weight ratio between SiO.sub.2 and ZrO.sub.2 is comprised between 99 and 0.7. The tetragonal ZrO.sub.2 phase is stable at temperatures comprised between 0.degree. and 1500.degree. C. The process comprises the preparation of: a solution of Zr(OR.sup.1).sub.4, where R.sup.1 is an alkyl or aryl, Si(OR.sup.2).sub.4, where R.sup.2 is alkyl or aryl, an organic solvent and an acid. Vapor-phase water is bubbled in said solution, then the obtained solution is drawn and the fibers thus obtained are heated to obtain the crystallization of ZrO.sub.2 and SiO.sub.2.

摘要翻译： 本发明涉及ZrO 2和SiO 2基陶瓷纤维，其中SiO2和ZrO2之间的重量比在99和0.7之间。 该方法包括制备：Zr（OR1）4的溶液，其中R1是烷基或芳基，Si（OR2）4，其中R2是 烷基或芳基，有机溶剂和酸。 将所述溶液中的气相水鼓泡，然后将得到的溶液拉伸，将得到的纤维加热，得到ZrO 2和SiO 2的结晶。