公开(公告)号：US08097447B2

公开(公告)日：2012-01-17

申请号：US12240043

申请日：2008-09-29

申请人： Gnanasekaran Gopalsamy ,  Kalpana Haresh Mody ,  Sumitra Datta ,  Bhavanath Jha

发明人： Gnanasekaran Gopalsamy ,  Kalpana Haresh Mody ,  Sumitra Datta ,  Bhavanath Jha

IPC分类号： C12N1/20 ,  C12Q1/04 ,  C12Q1/00

CPC分类号： C12N1/20 ,  C12Q1/045

摘要： The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. The media composition consists of 5-15 g of carbon source, 2.5-10 g of peptone, 2.5-10 g of yeast extract, 0.5-1.5 g of dipotassium hydrogen phosphate; 0.1-0.5 g of magnesium sulphate heptahydrate, 30 μl-4 ml of super saturated solution of sodium hydroxide, 5-20 g of potassium chloride and 10-30 g of κ-carrageenan in one liter of distilled water. The potassium salt in combination with κ-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria.

摘要翻译： 本发明涉及具有碱性pH的固体营养培养基组合物，其用于分离和鉴定纯形式的嗜碱性微生物。 培养基组成由5-15g碳源，2.5-10g蛋白胨，2.5-10g酵母提取物，0.5-1.5g磷酸氢二钾组成; 0.1-0.5g七水合硫酸镁，30μl-4ml超饱和氢氧化钠溶液，5-20g氯化钾和10-30g＆amp;角叉菜胶在1升蒸馏水中的溶液。 已经发现与特定比例的钾盐与卡拉胶的组合是在固化细菌培养基中的合适的琼脂替代物，特别是用于分离极碱性碱性物质。 本发明还提供了使用具有碱性pH的固体营养培养基组合物来研究可培养的嗜碱细菌的生物多样性的方法。