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1.发明授权Cyclic peptide and method of use for inducing an immunological response to hepatitis B virus 失效循环肽和用于诱导对乙型肝炎病毒的免疫应答的方法
公开(公告)号：US4778784A
公开(公告)日：1988-10-18
申请号：US124419
申请日：1987-11-23
申请人： Gordon R. Dreesman , James T. Sparrow , Darrell L. Peterson , Frederick B. Hollinger , Joseph L. Melnick
发明人： Gordon R. Dreesman , James T. Sparrow , Darrell L. Peterson , Frederick B. Hollinger , Joseph L. Melnick
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122 , Y10S930/223 , Y10S930/26
摘要： A composition for eliciting production of antibody to hepatitis B surface antigen and method of neutralizing the infectivity of hepatitis type B virus. A cyclic polypeptide is prepared having a disulfide bond in a hydrophilic region, namely residues 117-137 or 122-137 and an amino acid sequence unique from that associated with the native 25,000 molecular weight polypeptide derived from hepatitis B surface antigen.
摘要翻译： 用于引发乙型肝炎表面抗原抗体产生的组合物和中和B型肝炎病毒感染性的方法。 制备在亲水区域具有二硫键的环状多肽，即残基117-137或122-137以及与源于乙型肝炎表面抗原的天然25,000分子量多肽相关的氨基酸序列。
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公开(公告)号：US4956357A
公开(公告)日：1990-09-11
申请号：US842346
申请日：1986-03-28
申请人： Bruce S. Keenan , James T. Sparrow
发明人： Bruce S. Keenan , James T. Sparrow
IPC分类号： A61K31/565 , C07J1/00
CPC分类号： C07J1/0025
摘要： Disclosed is synthesized dihydrotestosterone heptanoate, its method of synthesis, and its method of use as an androgenic hormone of male gynecomastia, as a hormonal probe, as well as other applications.
摘要翻译： 公开了合成的二氢睾酮庚酸酯，其合成方法及其作为雄性男子乳腺发育的雄激素激素的方法，作为激素探针，以及其他应用。
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公开(公告)号：US06344436B1
公开(公告)日：2002-02-05
申请号：US08584043
申请日：1996-01-08
申请人： Louis C. Smith , James T. Sparrow , Jochen Hauer , Martha P. Mims
发明人： Louis C. Smith , James T. Sparrow , Jochen Hauer , Martha P. Mims
IPC分类号： C07K1400
CPC分类号： A61K47/645
摘要： Peptide-macromolecule complexes for delivery of nucleic acid to a cell. The nucleic acid carrier includes a binding complex. The binding complex contains a binding moiety which noncovalently binds to the nucleic acid. The binding complex can also contain a binding moiety which is associated with a surface ligand, nuclear ligand or a lysis agent. These may be associated with the binding moiety by spacers. In addition, the carrier may include a nucleic acid with a combination of the above binding complexes or binding moieties.
摘要翻译： 用于将核酸递送至细胞的肽 - 大分子复合物。 核酸载体包括结合复合物。 结合复合物含有非共价结合核酸的结合部分。 结合复合物还可以含有与表面配体，核配体或裂解剂相关的结合部分。 这些可以通过间隔物与结合部分相关联。 此外，载体可以包括具有上述结合复合物或结合部分的组合的核酸。
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4.发明授权Polyamide resin and method for preparation of reagents for immunodiagnostic use 失效聚酰胺树脂及免疫诊断用试剂的制备方法
公开(公告)号：US5028675A
公开(公告)日：1991-07-02
申请号：US309914
申请日：1989-02-10
IPC分类号： A61K47/48 , C08F220/56 , G01N33/543
CPC分类号： A61K47/48176 , C08F220/56 , G01N33/54313
摘要： A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by cross-linking a dimethylacrylamide monomer by co-polymerization with a functional monomer in an aqueous solution, emulsifying the aqueous solution in an organic solvent and isolating the polyamide resin beads formed by adding an initiator and a promoter. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.
摘要翻译： 用于肽和蛋白质合成的聚酰胺树脂及其制备和使用方法。 聚酰胺树脂通过在水溶液中与官能单体共聚而交联二甲基丙烯酰胺单体来制备，将有机溶剂中的水溶液乳化并分离通过加入引发剂和助催化剂形成的聚酰胺树脂珠。 根据本领域已知的方法，将珠子用作肽和蛋白质合成的固相。 聚酰胺树脂和合成的肽或蛋白质的缀合物直接用于免疫测定或免疫，而不需要将肽或蛋白质与树脂分离并随后纯化。
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公开(公告)号：US5296572A
公开(公告)日：1994-03-22
申请号：US693960
申请日：1991-04-29
IPC分类号： A61K47/48 , C08F220/56 , G01N33/543 , C08F226/02
CPC分类号： A61K47/48246 , A61K47/48176 , C08F220/56 , G01N33/54313
摘要： A large pore polyamide resin is useful for large peptide and protein (protide) synthesis. A method of preparing the same comprises mixing a dimethylacrylamide monomer with an unsaturated or alkenoyl amine monomer, a cross-linker and water, homogeneously emulsifying the aqueous mixture with an organic solvent in the presence of an emulsifier, adjusting the pH of the aqueous mixture during polymerization to 6-8.5 to produce large pore resin beads, and isolating the beads. The beads may be used as a solid phase substrate for the synthesis of a polyamide/protide conjugate. The polyamide resin/protide conjugate may be used, without separation of the protide from the resin or subsequent purification, for immunizing mammals, including humans, against the protide, for affinity purifying immunological molecules binding to the protide, and for immunoassays.
摘要翻译： 大孔聚酰胺树脂可用于大肽和蛋白质（保护）合成。 其制备方法包括将二甲基丙烯酰胺单体与不饱和或烯酰胺单体，交联剂和水混合，在乳化剂存在下，用有机溶剂均匀乳化水性混合物，调节含水混合物的pH值 聚合至6-8.5以产生大孔树脂珠，并分离珠。 珠粒可以用作合成聚酰胺/保护结合物的固相底物。 可以使用聚酰胺树脂/保护物缀合物，不将树脂的保护剂或随后的纯化分离，用于免疫哺乳动物（包括人类）抵抗抗原，用于亲和纯化免疫分子与抗原结合并进行免疫测定。
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6.发明授权Polyamide resin and method for preparation of reagents for immunodiagnostic use 失效聚酰胺树脂及免疫诊断用试剂的制备方法
公开(公告)号：US4973638A
公开(公告)日：1990-11-27
申请号：US368708
申请日：1989-06-19
IPC分类号： A61K47/48 , C08F220/56 , G01N33/543
CPC分类号： A61K47/48176 , C08F220/56 , G01N33/54313
摘要： A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by mixing a dimethylacrylamide monomer with an N-acrylyl-diaminoalkane functional monomer in an aqueous solution together with a cross-linker and emulsifying the aqueous solution in an organic solvent. An initiator and a promoter are added to polymerize the N-acrylyl-diaminoalkane functional monomer, dimethylacrylamide monomer, and cross-linker in the form of beads. The pH of the mixture is controlled during the polymeriztion. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immuniaztion without the need for separation of the peptide or protein from the resin and subsequent purification.
摘要翻译： 用于肽和蛋白质合成的聚酰胺树脂及其制备和使用方法。 聚酰胺树脂通过将二甲基丙烯酰胺单体与N-丙烯酰基 - 二氨基烷基官能单体一起在水溶液中与交联剂一起混合并将水溶液乳化在有机溶剂中来制备。 加入引发剂和促进剂以使珠子形式的N-丙烯酰基 - 二氨基烷基官能单体，二甲基丙烯酰胺单体和交联剂聚合。 在聚合过程中控制混合物的pH值。 根据本领域已知的方法，将珠子用作肽和蛋白质合成的固相。 聚酰胺树脂和合成的肽或蛋白质的缀合物直接用于免疫测定或免疫测定，而不需要将肽或蛋白质与树脂分离并随后纯化。
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公开(公告)号：US5512648A
公开(公告)日：1996-04-30
申请号：US169718
申请日：1993-12-16
IPC分类号： A61K47/48 , C08F220/56 , G01N33/543 , C08F226/02
CPC分类号： A61K47/48246 , A61K47/48176 , C08F220/56 , G01N33/54313
摘要： A large pore polyamide resin is useful for large peptide and protein (protide) synthesis. A method of preparing the same comprises mixing a dimethylacrylamide monomer with an unsaturated or alkenoyl amine monomer, a cross-linker and water, homogeneously emulsifying the aqueous mixture with an organic solvent in the presence of an emulsifier, adjusting the pH of the aqueous mixture during polymerization to 6-8.5 to produce large pore resin beads, and isolating the beads. The beads may be used as a solid phase substrate for the synthesis of a polyamide/protide conjugate. The polyamide resin/protide conjugate may be used, without separation of the protide from the resin or subsequent purification, for immunizing mammals, including humans, against the protide, for affinity purifying immunological molecules binding to the protide, and for immunoassays.
摘要翻译： 大孔聚酰胺树脂可用于大肽和蛋白质（保护）合成。 其制备方法包括将二甲基丙烯酰胺单体与不饱和或烯酰胺单体，交联剂和水混合，在乳化剂存在下，用有机溶剂均匀乳化水性混合物，调节含水混合物的pH值 聚合至6-8.5以产生大孔树脂珠，并分离珠。 珠粒可以用作合成聚酰胺/保护结合物的固相底物。 可以使用聚酰胺树脂/保护物缀合物，不将树脂的保护剂或随后的纯化分离，用于免疫哺乳动物（包括人类）抵抗抗原，用于亲和纯化免疫分子与抗原结合并进行免疫测定。
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8.发明授权Methods and compositions for the preparation and use of site-directed immunologic reagents 失效用于制备和使用定点免疫试剂的方法和组合物
公开(公告)号：US5126399A
公开(公告)日：1992-06-30
申请号：US368713
申请日：1989-06-20
IPC分类号： A61K38/00 , A61K39/00 , A61K47/48 , C07K14/00 , C07K14/02 , C07K14/09 , C07K14/16 , C07K14/445 , C07K14/82 , C07K19/00 , C08F220/56 , C12N9/12 , C12N9/16 , G01N33/531 , G01N33/543 , G01N33/569 , G01N33/68
CPC分类号： C07K14/005 , A61K47/48176 , C07K14/00 , C07K14/445 , C07K14/82 , C07K19/00 , C08F220/56 , C12N9/12 , C12N9/16 , G01N33/531 , G01N33/54313 , G01N33/56972 , G01N33/6878 , A61K38/00 , A61K39/00 , C12N2730/10122 , C12N2740/16222 , C12N2770/32122
摘要： Disclosed are peptidyl-resin conjugates made up of an immunogenic/antigenic peptide conjugated to a polyamide resin, wherein the peptide incorporates a helper T-cell epitope. The inclusion of a T-cell epitope in this peptide sequence provides particular benefits in the preparation of site-directed reagents intended as immunogens. In exemplary studies, a synthetic peptide predicted from Abelson murine leukemia virus abl oncogene (residues 389-403) was synthesized with a T-cell active epitope of 7 amino acids placed at its N-terminus (T-abl-resin). The T-abl-resin construct was found to greatly stimulate the immune response giving significantly higher specific antibody titers than abl-resin controls.
摘要翻译： 公开了由与聚酰胺树脂缀合的免疫原性/抗原肽组成的肽基 - 树脂缀合物，其中肽包含辅助T细胞表位。 在该肽序列中包含T细胞表位在制备用作免疫原的定点试剂中提供了特别的益处。 在示例性研究中，从放置于其N-末端（T-abl-树脂）的7个氨基酸的T细胞活性表位合成从Abelson鼠白血病病毒abl致癌基因（残基389-403）预测的合成肽。 发现T-abl树脂构建体大大刺激免疫应答，比abl-树脂对照提供显着更高的特异性抗体滴度。
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公开(公告)号：US5084509A
公开(公告)日：1992-01-28
申请号：US614857
申请日：1990-11-16
IPC分类号： A61K47/48 , C08F220/56 , G01N33/543
CPC分类号： A61K47/48176 , C08F220/56 , G01N33/54313
摘要： A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by mixing a dimethylacrylamide monomer with an N-acrylyl-diaminoalkane functional monomer in an aqueous solution together with a cross-linker and emulsifying the aqueous solution in an organic solvent. An initiator and a promoter are added to polymerize the N-acrylyl-diaminoalkane functional monomer, dimethylacrylamide monomer, and cross-linker in the form of beads. The pH of the mixture is controlled during the polymerization. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.
摘要翻译： 用于肽和蛋白质合成的聚酰胺树脂及其制备和使用方法。 聚酰胺树脂通过将二甲基丙烯酰胺单体与N-丙烯酰基 - 二氨基烷基官能单体一起在水溶液中与交联剂一起混合并将水溶液乳化在有机溶剂中来制备。 加入引发剂和促进剂以使珠子形式的N-丙烯酰基 - 二氨基烷基官能单体，二甲基丙烯酰胺单体和交联剂聚合。 在聚合期间控制混合物的pH。 根据本领域已知的方法，将珠子用作肽和蛋白质合成的固相。 聚酰胺树脂和合成的肽或蛋白质的缀合物直接用于免疫测定或免疫，而不需要从树脂中分离肽或蛋白质并随后的纯化。
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