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公开(公告)号:US20070259330A1
公开(公告)日:2007-11-08
申请号:US11739890
申请日:2007-04-25
申请人: Gregory Daryll Goddard , Mohan Mark Amaratunga , Cyndy D. Sanberg , James van Alstine , Oleg Kuzmenok
发明人: Gregory Daryll Goddard , Mohan Mark Amaratunga , Cyndy D. Sanberg , James van Alstine , Oleg Kuzmenok
CPC分类号: C12N5/0605
摘要: The present invention relates to the isolation of human stem cells for use e.g. in cell therapy. More specifically, the invention is a method of separating mononuclear cells from human blood, which method comprises providing a human blood sample together with density gradient media (DGM) in a container; spinning the container comprising blood and DGM; and collecting the DGM fraction that comprises mononuclear cells; wherein the DGM has a density which is >1.080 and
摘要翻译: 本发明涉及人类干细胞的分离,例如使用。 在细胞疗法中。 更具体地说,本发明是从人血液中分离单核细胞的方法,该方法包括在容器中提供人血液样品以及密度梯度介质(DGM); 旋转包含血液和DGM的容器; 并收集包含单核细胞的DGM级分; 其中DGM具有在25℃下测量的> 1.080和<1.090G / CM 3的密度。本发明还涉及包含密度梯度介质的袋和管,其中密度和重量摩尔渗透压浓度具有 已被优化用于本方法,优选用作试剂盒。
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公开(公告)号:US20110070596A1
公开(公告)日:2011-03-24
申请号:US12705828
申请日:2010-02-15
申请人: Nichole Lea Wood , Mohan Mark Amaratunga , Hans Grade , Faisal Ahmed Syud , Anup Sood , Reginald Donovan Smith , Ayse Betul Dinc , Amy Casey Williams , Anthony John Murray , Gregory Daryll Goddard
发明人: Nichole Lea Wood , Mohan Mark Amaratunga , Hans Grade , Faisal Ahmed Syud , Anup Sood , Reginald Donovan Smith , Ayse Betul Dinc , Amy Casey Williams , Anthony John Murray , Gregory Daryll Goddard
IPC分类号: G01N33/68 , C07D403/12 , G01N33/53
CPC分类号: G01N33/6845 , G01N33/6848
摘要: Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library.
摘要翻译: 提供了使用同位素标记的对称双功能交联剂和质谱定性和定量分析蛋白质复合物或蛋白质复合物的试剂和方法。 靶向部分,细胞渗透性部分或亲和部分可附加到双功能交联剂上。 同位素标记的对称双功能交联剂可用于试剂盒或文库。
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