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公开(公告)号：US20090035849A1
公开(公告)日：2009-02-05
申请号：US11983203
申请日：2007-11-06
申请人： Gregory E. Rice , Mark S. Baker , Michael Quinn
发明人： Gregory E. Rice , Mark S. Baker , Michael Quinn
CPC分类号： G01N30/14 , C07K1/22 , G01N33/6854 , G01N2030/009 , B01D15/3804
摘要： This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention.In one aspect, the invention provides a method of depleting a high-abundance molecule from a biological sample, comprising the steps of a) subjecting the sample to affinity depletion using an affinity support with high affinity for a high abundance molecule, and/or b) immunodepletion using an affinity support coupled to an antibody directed against whole or previously fractionated plasma or serum.
摘要翻译： 本发明涉及分析方法，特别涉及用于初步分级分离样品的方法，其中存在低丰度的目标分子，例如蛋白质，多糖或脂肪酸，以及更多丰富的很少或不感兴趣的分子。 特别地，本发明涉及从生物样品中消耗高丰度蛋白质的方法。 还公开了用于该方法的产品和试剂盒，并且构成了本发明的一部分。 一方面，本发明提供了从生物样品中消耗高丰度分子的方法，包括以下步骤：a）使用对高丰度分子具有高亲和力的亲和载体使样品亲和耗尽，和/或b ）使用与针对全部或先前分级的血浆或血清的抗体偶联的亲和载体的免疫缺损。
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公开(公告)号：US20110008900A1
公开(公告)日：2011-01-13
申请号：US12814387
申请日：2010-06-11
申请人： Gregory E. Rice , Mark S. Baker , Michael Quinn
发明人： Gregory E. Rice , Mark S. Baker , Michael Quinn
CPC分类号： G01N30/14 , C07K1/22 , G01N33/6854 , G01N2030/009 , B01D15/3804
摘要： This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention. In one aspect, the invention provides a method of depleting a high-abundance molecule from a biological sample, comprising the steps of a) subjecting the sample to affinity depletion using an affinity support with high affinity for a high abundance molecule, and/or b) immunodepletion using an affinity support coupled to an antibody directed against whole or previously fractionated plasma or serum.
摘要翻译： 本发明涉及分析方法，特别涉及用于初步分级分离样品的方法，其中存在低丰度的目标分子，例如蛋白质，多糖或脂肪酸，以及更多丰富的很少或不感兴趣的分子。 特别地，本发明涉及从生物样品中消耗高丰度蛋白质的方法。 还公开了用于该方法的产品和试剂盒，并且构成了本发明的一部分。 一方面，本发明提供了从生物样品中消耗高丰度分子的方法，包括以下步骤：a）使用对高丰度分子具有高亲和力的亲和载体使样品亲和耗尽，和/或b ）使用与针对全部或先前分级的血浆或血清的抗体偶联的亲和载体的免疫缺损。
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公开(公告)号：US09417218B2
公开(公告)日：2016-08-16
申请号：US12814387
申请日：2010-06-11
申请人： Gregory E. Rice , Michael Quinn
发明人： Gregory E. Rice , Mark S. Baker , Michael Quinn
IPC分类号： G01N33/543 , G01N30/14 , C07K1/22 , G01N33/68 , G01N30/00
CPC分类号： G01N30/14 , C07K1/22 , G01N33/6854 , G01N2030/009 , B01D15/3804
摘要： This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention. In one aspect, the invention provides a method of depleting a high-abundance molecule from a biological sample, comprising the steps of a) subjecting the sample to affinity depletion using an affinity support with high affinity for a high abundance molecule, and/or b) immunodepletion using an affinity support coupled to an antibody directed against whole or previously fractionated plasma or serum.
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