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公开(公告)号：US5786196A
公开(公告)日：1998-07-28
申请号：US490003
申请日：1995-06-12
申请人： Gregory L. Cote , Herbert Wyckoff , Peter Biely
发明人： Gregory L. Cote , Herbert Wyckoff , Peter Biely
CPC分类号： C12Y302/01084 , C12N9/1051 , C12N9/2402
摘要： A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.
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公开(公告)号：US5889179A
公开(公告)日：1999-03-30
申请号：US98368
申请日：1998-06-17
申请人： Gregory L. Cote , Herbert Wyckoff
发明人： Gregory L. Cote , Herbert Wyckoff
CPC分类号： C12Y302/01084 , C12N9/1051 , C12N9/2402
摘要： A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.
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公开(公告)号：US5888776A
公开(公告)日：1999-03-30
申请号：US98886
申请日：1998-06-17
申请人： Gregory L. Cote , Herbert Wyckoff , Peter Biely
发明人： Gregory L. Cote , Herbert Wyckoff , Peter Biely
CPC分类号： C12Y302/01084 , C12N9/1051 , C12N9/2402
摘要： A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.
摘要翻译： 描述了一种对交替糖内切水解有效的新型酶，交替糖酶，产生相对于天然交替糖显示出降低的粘度和增加的溶解度的低分子量级分的变薄的组合物。 通过从土壤分离的多种新型细菌在细胞外产生和分泌酶。 由交替糖酶水解产生的稀释的交替糖中存在的部分之一是环状四糖，环{-6）-β-D-Glcp-（1,3）-β-D-Glcp-（1,6） - α-D-Glcp-（1,3）-a-D-Glcp-（1-}。一种分离产生内源α-D-葡聚糖酶的微生物菌株的新方法，例如有效用于内切酶裂解的交替糖酶 还描述了交替糖的稀释，将受试菌株的培养物与偶联到可检测指示物的交替糖的测试底物接触。释放的指示剂的检测提供了内源α-D-葡聚糖酶活性的指示。
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