公开(公告)号：US11692188B2

公开(公告)日：2023-07-04

申请号：US17497899

申请日：2021-10-09

Applicant: Guilin University of Technology

Inventor： Xiaoli Wu ,  Ke Ren ,  Mengwu Wang ,  Zewen He ,  Shaoling Peng ,  Jichan Ning ,  Yuanli Liu

IPC: C12N11/14 ,  C01F17/276 ,  C12N9/02 ,  C12N9/26 ,  C12N9/08

CPC classification number: C12N11/14 ,  C01F17/276 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/2414 ,  C12Y110/03002 ,  C12Y111/01007 ,  C12Y302/01001 ,  C01P2002/82 ,  C01P2004/03

Abstract: The technical field of enzyme immobilization, and particularly, an organic-inorganic hybrid nanoflower and a preparation method thereof. The organic-inorganic hybrid nanoflower is a flower-like immobilized enzyme formed by self-assembly of a layered rare earth compound as an inorganic carrier and a biological enzyme as an organic component. The layered rare earth compound is Ln2(OH)5NO3·nH2O, where Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, or Y, and n=1.1-2.5. The biological enzyme is one or more of α-amylase, horseradish peroxidase, or laccase. A layered rare earth compound is used as the inorganic carrier for the organic biological enzyme to form the flower-like immobilized enzyme. The immobilized enzyme has better stability and higher catalytic performance when compared with a free enzyme.

公开(公告)号：US11174476B2

公开(公告)日：2021-11-16

申请号：US16991660

申请日：2020-08-12

Applicant: Guilin University of Technology

Inventor： Xiaoli Wu ,  Ke Ren ,  Mengwu Wang ,  Zewen He ,  Shaoling Peng ,  Jichan Ning ,  Yuanli Liu

IPC: C12N11/14 ,  C12N9/08 ,  C01F17/276 ,  C12N9/02 ,  C12N9/26

Abstract: The technical field of enzyme immobilization, and particularly, an organic-inorganic hybrid nanoflower and a preparation method thereof. The organic-inorganic hybrid nanoflower is a flower-like immobilized enzyme formed by self-assembly of a layered rare earth compound as an inorganic carrier and a biological enzyme as an organic component. The layered rare earth compound is Ln2(OH)5NO3·nH2O, where Ln is one or more of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, or Y, and n=1.1-2.5. The biological enzyme is one or more of α-amylase, horseradish peroxidase, or laccase. A layered rare earth compound is used as the inorganic carrier for the organic biological enzyme to form the flower-like immobilized enzyme. The immobilized enzyme has better stability and higher catalytic performance when compared with a free enzyme.

公开(公告)号：US11891712B2

公开(公告)日：2024-02-06

申请号：US17417507

申请日：2020-12-21

Applicant: GUILIN UNIVERSITY OF TECHNOLOGY

Inventor： Xiaoli Wu ,  Ke Ren ,  He Zhang ,  Zewen He ,  Yuanli Liu

IPC: C25D1/00 ,  C25D9/00 ,  C25D9/08 ,  C25D9/02

CPC classification number: C25D1/006 ,  C25D9/00 ,  C25D9/02 ,  C25D9/08

Abstract: A method for preparing organic-inorganic hybrid nanoflower by electrodeposition is provided, which relates to the technical field of enzyme immobilization. An aqueous solution of a rare earth nitrate is mixed with a biological enzyme and a nitrate to obtain a mixed solution; the rare earth ions in the rare earth nitrate are one or more selected from La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Yb and Y ions; the biological enzyme is α-amylase, horseradish peroxidase or laccase; then, the mixed solution is electrodeposited with a three-electrode system consisting of a working electrode, a counter electrode and a reference electrode to obtain an electrodeposited film on the surface of the working electrode; thereafter, the electrodeposited film is washed and dried successively to obtain organic-inorganic hybrid nanoflower.