公开(公告)号：US20240319203A1

公开(公告)日：2024-09-26

申请号：US18579620

申请日：2022-08-04

申请人： H. LUNDBECK A/S

发明人： Anne-Marie JACOBSEN ,  Line Rorbaek OLSEN ,  Frank SCHALK ,  Nico van de MERBEL

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N33/6842 ,  G01N33/6896 ,  G01N2800/2821 ,  G01N2800/52

摘要： The present invention relates to a method of measuring pS396 Tau in a sample comprising the steps of i-iv, i) Treating a CSF sample from a subject suffering or suspected to be suffering from a Tau pathology with trypsin, ii) Subjecting the sample in step i) to a desphosphorylation agent and subsequently take part of the sample aside for step iv) before proceeding to step iii) with the remaining sample, iii) Subjecting the dephosphorylated sample from step ii) to a second trypsin treatment, iv) Measuring the amount of the Tau peptides (SPVVSGDTSPR) corresponding to Tau residues 396-406 in the samples from step ii) and step iii) using LC-MS.