公开(公告)号：US12180533B2

公开(公告)日：2024-12-31

申请号：US16976267

申请日：2019-03-01

Applicant: HIROSHIMA UNIVERSITY

Inventor： Ryuichi Hirota ,  Akio Kuroda ,  Kei Motomura

IPC: C12Q1/06 ,  C07K14/195 ,  C12N9/02 ,  C12N15/74

Abstract: It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.