公开(公告)号：US11130981B2

公开(公告)日：2021-09-28

申请号：US16328560

申请日：2017-07-31

Applicant: HIROSHIMA UNIVERSITY

Inventor： Ryuichi Hirota ,  Akio Kuroda

IPC: A01N63/00 ,  C12Q1/02 ,  C12N1/20 ,  C12N15/70

Abstract: In order to provide a transformant by which a high containment effect is obtained and which can be prepared by a simple procedure, a method for producing the transformant, and a method for detecting the presence of a reduced phosphorous compound with use of the transformant, a transformant in accordance with an embodiment of the present invention is a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced.

公开(公告)号：US09499822B2

公开(公告)日：2016-11-22

申请号：US14413035

申请日：2013-08-08

Applicant: Hiroshima University

Inventor： Akio Kuroda ,  Ryuichi Hirota ,  Takenori Ishida

IPC: C12N15/52 ,  C12N9/02 ,  C07K14/195 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20

CPC classification number: C12N15/52 ,  C07K14/195 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0004 ,  C12Y120/01001

Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.

Abstract translation: 提供了一种用于选择性培养微生物的简单和便宜的方法，该方法使得即使不使用灭菌操作或抗生素也可选择性培养感兴趣的微生物。 根据本发明的选择性培养微生物的方法包括在含有磷酸作为唯一磷源的培养基中培养引入了亚磷酸脱氢酶基因的重组微生物的步骤。

公开(公告)号：US12180533B2

公开(公告)日：2024-12-31

申请号：US16976267

申请日：2019-03-01

Applicant: HIROSHIMA UNIVERSITY

Inventor： Ryuichi Hirota ,  Akio Kuroda ,  Kei Motomura

IPC: C12Q1/06 ,  C07K14/195 ,  C12N9/02 ,  C12N15/74

Abstract: It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.

公开(公告)号：US20150125934A1

公开(公告)日：2015-05-07

申请号：US14413035

申请日：2013-08-08

Applicant: Hiroshima University

Inventor： Akio Kuroda ,  Ryuichi Hirota ,  Takencri Ishida

IPC: C12N15/52 ,  C07K14/195 ,  C12N9/02

CPC classification number: C12N15/52 ,  C07K14/195 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0004 ,  C12Y120/01001

Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.

Abstract translation: 提供了一种用于选择性培养微生物的简单和便宜的方法，该方法使得即使不使用灭菌操作或抗生素也可选择性培养感兴趣的微生物。 根据本发明的选择性培养微生物的方法包括在含有磷酸作为唯一磷源的培养基中培养引入了亚磷酸脱氢酶基因的重组微生物的步骤。