公开(公告)号：US20240288372A1

公开(公告)日：2024-08-29

申请号：US18426589

申请日：2024-01-30

申请人： HITACHI, LTD.

发明人： Takahiro Ando ,  Takahide Yokoi ,  Chihiro Manri ,  Takeshi Ishida

IPC分类号： G01N21/64 ,  C12Q1/6806 ,  G01N27/447

CPC分类号： G01N21/6486 ,  C12Q1/6806 ,  G01N27/447

摘要： Provided is a gene analysis method including performing a single-base extension reaction using a primer for a single-base extension reaction for detection of a target nucleotide sequence, and a substrate for a single-base extension reaction having a fluorescent dye; subjecting a reaction product of the single-base extension reaction to electrophoresis; and measuring mobility of the electrophoresis and fluorescence intensity of the fluorescent dye, and detecting a wild type and a mutant of the target nucleotide sequence based on the fluorescence intensity. Also provided is a kit for use in the gene analysis method disclosed herein.