公开(公告)号：US20240084354A1

公开(公告)日：2024-03-14

申请号：US18252941

申请日：2021-11-15

申请人： HONING BIOSCIENCES ,  INSTITUT CURIE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

发明人： Franck PEREZ ,  Zelia GOUVEIA

IPC分类号： C12P21/00 ,  C07K14/36 ,  C07K14/54 ,  C07K14/55 ,  C12N15/85

CPC分类号： C12P21/005 ,  C07K14/36 ,  C07K14/5434 ,  C07K14/55 ,  C12N15/85 ,  C07K2319/04 ,  C07K2319/20 ,  C12N2740/15043 ,  C12N2830/002

摘要： The present invention relates to a polynucleotide comprising a gene encoding a hook protein and a gene encoding a protein of interest, said protein of interest being either a secretory protein or a cell membrane-anchored protein, wherein:



said gene encoding the hook protein is under the control of a first transcription-activating signal,
said gene encoding the protein of interest is under the control of a second transcription-activating signal, said second transcription-activating signal allowing a lower rate or frequency of transcription initiation than the first transcription-activating signal,
said hook protein is fused to a cellular compartment-retention peptide, and
said protein of interest is fused to a hook protein-binding domain




It also related to vectors comprising the polynucleotide, cells comprising the polynucleotide or the vector and compositions comprising the same. It further relates to methods and uses for modulating the secretion or cell membrane-anchorage of a protein of interest, or for preventing and/or treating a disease in a subject in need thereof.

公开(公告)号：US20240018197A1

公开(公告)日：2024-01-18

申请号：US18464507

申请日：2023-09-11

申请人： INSTITUT CURIE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

发明人： Franck PEREZ ,  Zelia GOUVEIA ,  Gaelle BONCOMPAIN

IPC分类号： C07K14/36 ,  C07K14/725 ,  C07K14/74 ,  C07K14/705 ,  C12N15/63

CPC分类号： C07K14/36 ,  C07K14/7051 ,  C07K14/70539 ,  C07K14/7056 ,  C12N15/63 ,  C07K2319/04 ,  C07K2319/10

摘要： A hook fusion protein, which includes a hook domain and at least one cytoplasmic carboxyl endoplasmic reticulum (ER) retention signal and/or at least one cytoplasmic amino terminal endoplasmic reticulum (ER) retention signal; wherein the hook fusion protein is a soluble protein that localizes in the cytoplasm. Also, a nucleic acid system for intracellular targeting control including a nucleic acid encoding a target fusion protein including a hook fusion protein, and a nucleic acid encoding a target fusion protein including a hook-binding domain; wherein the target fusion protein is a membrane protein; and wherein the hook fusion protein localizes in the ER when bound to the target fusion protein. Additionally, a vector system, viral particle system, host cell and kit include these nucleic acids. Further, the vector system, viral particle system, host cell or kit for use as a medicament, in particular for immunotherapy.