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公开(公告)号：US20150037812A1
公开(公告)日：2015-02-05
申请号：US14452428
申请日：2014-08-05
CPC分类号： G01N33/84 , C07K14/4728 , C07K2319/095 , C07K2319/21 , C07K2319/60 , C12N9/12 , G01N33/52 , G01N33/582
摘要： The presently-disclosed subject matter includes fluorescent protein-based indicators for detecting ions, small molecule analytes, or combinations thereof. In some embodiments the indicators include a polypeptide, which itself includes a fluorescent polypeptide, a compound-binding polypeptide, and a polypeptide target of the compound-binding polypeptide. In some embodiments the polypeptide includes an EosFP polypeptide, a calmodulin polypeptide, and a M13 polypeptide, or fragments and/or variants thereof. The presently-disclosed subject matter also includes methods for detecting calcium in a sample with embodiments of the present polypeptides. In some embodiments the present indicators experience a permanent shift from green to red fluorescent when exposed to an detecting substance, such as calcium.
摘要翻译： 目前公开的主题包括用于检测离子的荧光蛋白质指示剂，小分子分析物或其组合。 在一些实施方案中，指示剂包括本身包含化合物结合多肽的荧光多肽，化合物结合多肽和多肽靶的多肽。 在一些实施方案中，多肽包括EosFP多肽，钙调素多肽和M13多肽，或其片段和/或变体。 目前公开的主题还包括用本发明多肽的实施方案检测样品中的钙的方法。 在一些实施方案中，当暴露于检测物质例如钙时，本指标经历从绿色到红色荧光的永久性转变。
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公开(公告)号：US09518996B2
公开(公告)日：2016-12-13
申请号：US14452428
申请日：2014-08-05
CPC分类号： G01N33/84 , C07K14/4728 , C07K2319/095 , C07K2319/21 , C07K2319/60 , C12N9/12 , G01N33/52 , G01N33/582
摘要： The presently-disclosed subject matter includes fluorescent protein-based indicators for detecting ions, small molecule analytes, or combinations thereof. In some embodiments the indicators include a polypeptide, which itself includes a fluorescent polypeptide, a compound-binding polypeptide, and a polypeptide target of the compound-binding polypeptide. In some embodiments the polypeptide includes an EosFP polypeptide, a calmodulin polypeptide, and a M13 polypeptide, or fragments and/or variants thereof. The presently-disclosed subject matter also includes methods for detecting calcium in a sample with embodiments of the present polypeptides. In some embodiments the present indicators experience a permanent shift from green to red fluorescent when exposed to an detecting substance, such as calcium.
摘要翻译： 目前公开的主题包括用于检测离子的荧光蛋白质指示剂，小分子分析物或其组合。 在一些实施方案中，指示剂包括本身包含化合物结合多肽的荧光多肽，化合物结合多肽和多肽靶的多肽。 在一些实施方案中，多肽包括EosFP多肽，钙调蛋白多肽和M13多肽，或其片段和/或变体。 目前公开的主题还包括用本发明多肽的实施方案检测样品中的钙的方法。 在一些实施方案中，当暴露于检测物质例如钙时，本指标经历从绿色到红色荧光的永久性转变。
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公开(公告)号：US10067148B2
公开(公告)日：2018-09-04
申请号：US15335707
申请日：2016-10-27
CPC分类号： G01N33/84 , C07K14/4728 , C07K2319/095 , C07K2319/21 , C07K2319/60 , C12N9/12 , G01N33/52 , G01N33/582
摘要： The presently-disclosed subject matter includes fluorescent protein-based indicators for detecting ions, small molecule analytes, or combinations thereof. In some embodiments the indicators include a polypeptide, which itself includes a fluorescent polypeptide, a compound-binding polypeptide, and a polypeptide target of the compound-binding polypeptide. In some embodiments the polypeptide includes an EosFP polypeptide, a calmodulin polypeptide, and a M13 polypeptide, or fragments and/or variants thereof. The presently-disclosed subject matter also includes methods for detecting calcium in a sample with embodiments of the present polypeptides. In some embodiments the present indicators experience a permanent shift from green to red fluorescent when exposed to an detecting substance, such as calcium.
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公开(公告)号：US10041935B2
公开(公告)日：2018-08-07
申请号：US14451998
申请日：2014-08-05
摘要： This disclosure provides methods for identification of olfactory sensosry neurons (OSN) that recognize specific odorants. The method comprises introducing into the OSN, a permanent activity marker which exhibits a detectable and permanent change upon activation of the neuron in response to an odorant and exposure to an exteranal stimulus, such as light. The OSN can be isolated and its receptor characterized.
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