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    Ion-activated photolabile compounds
    2.
    发明授权
    Ion-activated photolabile compounds 有权

    公开(公告)号：US10407399B2

    公开(公告)日：2019-09-10

    申请号：US15756788

    申请日：2016-09-01

    申请人： HOWARD HUGHES MEDICAL INSTITUTE

    发明人： Luke D. Lavis ,  Jonathan B. Grimm ,  Laurel M. Heckman ,  Eric R. Schreiter

    IPC分类号： C07D311/16 ,  C07D307/94 ,  C07D311/82 ,  C07D405/12 ,  A61N5/06 ,  A61K41/00 ,  C09K11/06 ,  G01N21/64 ,  G01N33/58 ,  G01N33/84

    摘要： The presently-disclosed subject matter relates to analyte-activated photolabile compounds. The compounds include the formula: wherein Z includes a maskable molecule; L is selected from a bond, C, C(O), O, alkyl, (O)alkyl, and alkoxy; R1 is selected from H, halogen, alkyl, and acyl; each R2 is independently selected from H, alkyl, aryl, and acyl, and is optionally substituted with one or more heteroatoms independently selected from COOH and COO(alkyl); and R3 is selected from H, alkyl, aryl, halogen, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), amine, CHO, COOH, COO(alkyl), COO(aryl), PO3H2, and SO3H, and is optionally substituted with one or more heteroatoms independently selected from N, O, and S, halogen, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), amine, NO2, CHO, COO, COOH, COO(alkyl), COO(aryl), C(O)NR2, PO3H2, and/or SO3H. Also provided are methods of detecting calcium and treating a subject with the analyte-activated photolabile compounds.

    Voltage indicators
    7.
    发明授权
    Voltage indicators 有权

    公开(公告)号：US11598792B2

    公开(公告)日：2023-03-07

    申请号：US16872188

    申请日：2020-05-11

    申请人： HOWARD HUGHES MEDICAL INSTITUTE

    发明人： Eric R. Schreiter ,  Ahmed Abdelfattah

    IPC分类号： G01R15/22 ,  G01R19/00 ,  C07K14/435 ,  G01N21/64

    摘要： A voltage indicator includes a polypeptide sequence comprising a voltage-sensitive opsin domain and a capture protein domain arranged and disposed to capture a fluorescent dye ligand. When the fluorescent dye ligand is captured and the voltage indicator is bound to a cell membrane, an increase in voltage across the cell membrane causes an increase in fluorescent emission.

    FLUORESCENT PROTEIN-BASED INDICATORS
    8.
    发明申请
    FLUORESCENT PROTEIN-BASED INDICATORS 有权
    基于荧光蛋白的指示剂

    公开(公告)号：US20150037812A1

    公开(公告)日：2015-02-05

    申请号：US14452428

    申请日：2014-08-05

    申请人： Howard Hughes Medical Institute

    发明人： Eric R. Schreiter ,  Loren L. Looger ,  Benjamin F. Fosque

    IPC分类号： G01N33/84 ,  C12N9/12 ,  G01N33/58 ,  C07K14/47

    CPC分类号： G01N33/84 ,  C07K14/4728 ,  C07K2319/095 ,  C07K2319/21 ,  C07K2319/60 ,  C12N9/12 ,  G01N33/52 ,  G01N33/582

    摘要： The presently-disclosed subject matter includes fluorescent protein-based indicators for detecting ions, small molecule analytes, or combinations thereof. In some embodiments the indicators include a polypeptide, which itself includes a fluorescent polypeptide, a compound-binding polypeptide, and a polypeptide target of the compound-binding polypeptide. In some embodiments the polypeptide includes an EosFP polypeptide, a calmodulin polypeptide, and a M13 polypeptide, or fragments and/or variants thereof. The presently-disclosed subject matter also includes methods for detecting calcium in a sample with embodiments of the present polypeptides. In some embodiments the present indicators experience a permanent shift from green to red fluorescent when exposed to an detecting substance, such as calcium.

    摘要翻译： 目前公开的主题包括用于检测离子的荧光蛋白质指示剂，小分子分析物或其组合。 在一些实施方案中，指示剂包括本身包含化合物结合多肽的荧光多肽，化合物结合多肽和多肽靶的多肽。 在一些实施方案中，多肽包括EosFP多肽，钙调素多肽和M13多肽，或其片段和/或变体。 目前公开的主题还包括用本发明多肽的实施方案检测样品中的钙的方法。 在一些实施方案中，当暴露于检测物质例如钙时，本指标经历从绿色到红色荧光的永久性转变。