公开(公告)号：US20160265036A1

公开(公告)日：2016-09-15

申请号：US15034831

申请日：2014-11-05

Applicant: HTG MOLECULAR DIAGNOSTICS, INC.

Inventor： Matt Rounseville ,  Vijay Modur

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6834 ,  C12Q2521/327 ,  C12Q2521/501 ,  C12Q2521/514 ,  C12Q2525/161 ,  C12Q2533/107 ,  C12Q2537/125 ,  C12Q2561/125 ,  C12Q2565/519 ,  C12Q2521/319

Abstract: Disclosed herein are methods for detecting a target nucleic acid molecule in a sample. The methods can include contacting a sample with a detectably labeled probe (detection probe) that specifically binds to a first target sequence in the target nucleic acid molecule, a bifunctional oligonucleotide including a portion that specifically binds to a second target sequence in the target nucleic acid molecule and a portion that specifically binds to an anchor, and a surface comprising the anchor. Specifically bound detection probe and bifunctional oligonucleotide are ligated and a reagent that specifically removes substantially all of the target nucleic acid is added. Unligated detection probe is removed and presence of the detectable label is detected. In other embodiments, the ligation and/or removal of target nucleic acid are omitted and the detection probe specifically bound to the target nucleic acid is detected.

Abstract translation: 本文公开了用于检测样品中靶核酸分子的方法。 所述方法可以包括将样品与特异性结合靶核酸分子中的第一靶序列的可检测标记的探针（检测探针）接触，双功能寡核苷酸，包括特异性结合靶核酸中的第二靶序列的部分 分子和特异性结合锚的部分，以及包含锚的表面。 连接特异性结合的检测探针和双功能寡核苷酸，并添加特异性去除基本上所有靶核酸的试剂。 去除未螯合的检测探针，检测到可检测标记的存在。 在其它实施方案中，省略靶核酸的连接和/或去除，并检测特异性结合靶核酸的检测探针。

公开(公告)号：US20160019337A1

公开(公告)日：2016-01-21

申请号：US14772038

申请日：2014-03-03

Applicant: HTG MOLECULAR DIAGNOSTICS, INC.

Inventor： Christopher Roberts ,  Hui Wang ,  Zhenquiang Lu ,  Krishna Maddula ,  Sam Rua ,  Kevin Knapp ,  Byron Lawson ,  Debrah Thompson ,  Michael Hrubiak ,  Tyler Breedlove ,  Vijay Modur

IPC: G06F19/20 ,  C12Q1/68

CPC classification number: G16B25/00 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/57423

Abstract: This disclosure concerns the identification of biomarkers that are characteristic of squamous or non squamous (e.g., adenocarcinoma, large cell carcinoma, carcinoid tumor, sarcomatoid carcinoma) subtypes of non small cell lung cancer (NSCLC), clinically useful NSCLC classifiers, kits and arrays for distinguishing squamous and nonsquamous NSCLC subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing.

Abstract translation: 本公开涉及鉴定非小细胞肺癌（NSCLC）的鳞状或非鳞状体（例如，腺癌，大细胞癌，类癌瘤，肉瘤样癌）亚型，临床上有用的NSCLC分类器，试剂盒和阵列的特征性生物标志物 区分鳞状和非鳞状NSCLC亚型，用于确定临床有用的分类器的生物信息学方法，以及上述每一种的使用方法。