公开(公告)号：US11268133B2

公开(公告)日：2022-03-08

申请号：US16377650

申请日：2019-04-08

申请人： HTG Molecular Diagnostics, Inc.

发明人： Bruce A. Seligmann ,  BJ Kerns ,  John Luecke ,  Matt Rounseville ,  Ihab Botros ,  Mark Schwartz

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

摘要： Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.

公开(公告)号：US20190226013A1

公开(公告)日：2019-07-25

申请号：US16377650

申请日：2019-04-08

申请人： HTG Molecular Diagnostics, Inc.

发明人： Bruce A. Seligmann ,  BJ Kerns ,  John Luecke ,  Matt Rounseville ,  Ihab Botros ,  Mark Schwartz

IPC分类号： C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

摘要： Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.

公开(公告)号：US20140243238A1

公开(公告)日：2014-08-28

申请号：US14347855

申请日：2012-09-26

申请人： HTG MOLECULAR DIAGNOSTICS, INC.

发明人： Bruce A. Seligmann ,  Matt Rounseville ,  Krishna Maddula ,  Ihab Botros ,  Chris Cox

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2521/325 ,  C12Q2525/207 ,  C12Q2537/143 ,  C12Q2561/108

摘要： Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.

摘要翻译： 本文公开了共检测样品中靶信使RNA（mRNA）和小非编码RNA（例如miRNA）的存在的方法。 所公开的方法可以用于在单个测定中（例如在多孔测定的相同反应或相同的孔中）同时检测mRNA和小的非编码RNA。 所述方法可以包括使样品与多个核酸酶保护探针接触，所述核酸酶保护探针包括至少一个特异性结合靶mRNA的探针和至少一个特异性结合靶小编码RNA的探针，使样品与核酸酶特异性接触 对于单链核酸，并且例如在微阵列上检测NPP。