公开(公告)号：US11268133B2

公开(公告)日：2022-03-08

申请号：US16377650

申请日：2019-04-08

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Bruce A. Seligmann ,  BJ Kerns ,  John Luecke ,  Matt Rounseville ,  Ihab Botros ,  Mark Schwartz

IPC: C12Q1/68 ,  C07H21/04 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.

公开(公告)号：US20190226013A1

公开(公告)日：2019-07-25

申请号：US16377650

申请日：2019-04-08

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Bruce A. Seligmann ,  BJ Kerns ,  John Luecke ,  Matt Rounseville ,  Ihab Botros ,  Mark Schwartz

IPC: C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.