公开(公告)号：US20150344897A1

公开(公告)日：2015-12-03

申请号：US14422661

申请日：2012-09-14

申请人： Haibo Wang ,  Fushuang Dong ,  Mengyu Lv ,  Yanmin Zhang ,  Zhiheng Ren ,  Fan Yang ,  Xinchao Liang ,  Wenbo Zuo ,  Xueping Shi ,  Huanhuan Zhang ,  Yiping Gao ,  He Zhao ,  Xian Xu ,  Guozhong Sun ,  Jianfang Chai ,  Yongwei Liu ,  Jinyong Zhu ,  Qiufen Han ,  Qiang Zhang ,  Huijie Ma ,  Zhanwu Wang ,  Junfeng Guan

发明人： Haibo Wang ,  Fushuang Dong ,  Mengyu Lv ,  Yanmin Zhang ,  Zhiheng Ren ,  Fan Yang ,  Xinchao Liang ,  Wenbo Zuo ,  Xueping Shi ,  Huanhuan Zhang ,  Yiping Gao ,  He Zhao ,  Xian Xu ,  Guozhong Sun ,  Jianfang Chai ,  Yongwei Liu ,  Jinyong Zhu ,  Qiufen Han ,  Qiang Zhang ,  Huijie Ma ,  Zhanwu Wang ,  Junfeng Guan

IPC分类号： C12N15/82

CPC分类号： C12N15/8205 ,  C12N15/8207

摘要： The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively.

摘要翻译： 本发明是通过足够和微伤（SMW）的单子叶植物的茎顶分生组织转化的方法。 技术过程包括：通过在种子发芽1-2天后枝条生长至0.2-2厘米时，通过去除胚芽鞘来揭开顶端分生组织; 使用具有直径为4-20μm，暴露长度为0.5-3mm的直径为4-20μm的100-5000个刷毛的SMW刷，通过刺入和刷刷2-3次来对顶端分生组织进行充分和微小的伤口转化，并浸渍 含有包含外源基因的二元载体的根癌农杆菌; 共培养后将经处理的分生组织直接发育至正常植物; 促进植物发展大穗，种植更多种子; 分别收获T0植物的种子; 检测和识别从每个T0植物繁殖的T1代转化结果。 本发明的优点独立于组织培养，基因型无限，不需携带抗性标记，简单大规模进行，适用于可以设置种子的所有单子叶植物。 使用该方法对小麦，水稻和玉米的转化效率分别为49％，66.3％和100％。

公开(公告)号：US20160135379A1

公开(公告)日：2016-05-19

申请号：US14422650

申请日：2012-09-14

申请人： Haibo Wang ,  Mengyu Lv ,  Fushuang Dong ,  Yanmin Zhang ,  Zhiheng Ren ,  Fan Yang ,  Guozhong Sun ,  Xian Xu ,  Xueping Shi ,  Huanhuan Zhang ,  He Zhao ,  Jianfang Chai ,  Yiping Gao ,  Yongwei Liu ,  Jinyong Zhu ,  Qiufen Han ,  Qiang Zhang ,  Huijie Ma ,  Zhanwu Wang ,  Junfeng Guan

发明人： Haibo Wang ,  Mengyu Lv ,  Fushuang Dong ,  Yanmin Zhang ,  Zhiheng Ren ,  Fan Yang ,  Guozhong Sun ,  Xian Xu ,  Xueping Shi ,  Huanhuan Zhang ,  He Zhao ,  Jianfang Chai ,  Yiping Gao ,  Yongwei Liu ,  Jinyong Zhu ,  Qiufen Han ,  Qiang Zhang ,  Huijie Ma ,  Zhanwu Wang ,  Junfeng Guan

IPC分类号： A01G1/00

CPC分类号： A01G22/00 ,  C12N15/8205 ,  C12N15/8207

摘要： The present invention is a method of shoot apical meristem transformation for dicot plant via sufficient and micro wounding (SMW). The technical process includes: germinate the seeds in Petri dish or in the nutrient matrix which can be transplanted with the seedling; expose the shoot apical meristem by removing one cotyledon away; make sufficient and micro wounding transformation treatment in vivo to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; after co-cultivation, develop the treated objects directly to normal plants in the nutrient matrix and then transplant the matrix and seedling together into pot or field; promote the plant to develop more branches, pods, bolls, fruits, and seeds; harvest the seeds of each branch of individual T0 plants separately; detect and identify the transformation results in T1 generation from every T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, no need to graft, unnecessary to carry resistant marker, high survival rate in transplantation and seedlings recovered rapidly, easy to manipulate and large scale to perform, and applicable to all dicot plants which can set seeds. The transformation efficiencies for cotton and soybean using this method are 50% and 76.5%, respectively.

摘要翻译： 本发明是通过足够和微伤（SMW）对双子叶植物的茎顶分生组织转化的方法。 技术过程包括：培养培养皿中的种子或可以移植苗的营养基质; 通过去除一个子叶暴露出芽顶端分生组织; 通过使用具有100-5000根直径为4-20μm的刷毛的SMW刷子，每次暴露的长度为0.5-3mm，通过刺激和刷刷2-3次，使其在顶端分生组织中进行充分和微创伤转化处理至顶端分生组织 并与含有外源基因的含有双元载体的根癌土壤杆菌浸渍; 共培养后，将处理过的物品直接植入营养基质中的正常植物，然后将基质和幼苗移植到盆中或田间; 促进植物开发更多的分支，荚果，棉铃，水果和种子; 分别收获各个T0植物各枝的种子; 检测并确定每个T0工厂的T1代转化结果。 本发明的优点独立于组织培养，基因型无限，无需移植，不需携带抗性标记，移植中高存活率和快速恢复苗，易于操作和大规模执行，适用于所有双子菜 可以种植种子的植物。 使用这种方法的棉花和大豆的转化效率分别为50％和76.5％。

公开(公告)号：US20120172212A1

公开(公告)日：2012-07-05

申请号：US12408700

申请日：2009-03-21

申请人： Jun Yue ,  Xia Chu ,  Liwei Jia ,  Jianbin Ou ,  Yang Liu ,  Yun Zhang ,  Pengfei Yu ,  Xian Xu ,  Jiaming Wang ,  Dezhi Zeng

发明人： Jun Yue ,  Xia Chu ,  Liwei Jia ,  Jianbin Ou ,  Yang Liu ,  Yun Zhang ,  Pengfei Yu ,  Xian Xu ,  Jiaming Wang ,  Dezhi Zeng

IPC分类号： B01J21/06

CPC分类号： B01D53/9445 ,  B01D2255/2092 ,  B01D2255/407 ,  B01D2255/9025 ,  B01J23/38 ,  B01J23/63 ,  B01J23/894 ,  B01J35/0006 ,  B01J35/04 ,  B01J35/1019 ,  B01J35/109 ,  B01J37/0244 ,  Y02A50/2324 ,  Y02T10/22

摘要： The present invention disclosed a tri-layer structured metal composite oxides material which used in a catalyst coat for purifying vehicle exhaust gas, and the method for manufacturing the same.

摘要翻译： 本发明公开了一种用于净化车辆废气的催化剂涂层中的三层结构金属复合氧化物材料及其制造方法。