公开(公告)号：US20080206787A1

公开(公告)日：2008-08-28

申请号：US11711946

申请日：2007-02-27

申请人： Haifeng M. Wu ,  Ming Jin ,  Michael G. Bissell ,  Spero R. Cataland

发明人： Haifeng M. Wu ,  Ming Jin ,  Michael G. Bissell ,  Spero R. Cataland

IPC分类号： G01N33/573

CPC分类号： G01N33/6851 ,  C12Q1/37 ,  G01N2333/96486

摘要： The present invention provides for various processes for determining enzyme activity. In one embodiment, a process for detecting an enzyme activity includes contacting a blood component with a substrate comprising a cleavage site of the enzyme and a tag moiety to produce a sample comprising a cleavage product having a known mass, and placing the sample in contact with a SELDI sample chip which has surface moieties which bind to the cleavage product. In another embodiment, the process further includes subjecting the SELDI sample chip to SELDI mass spectrometry to identify the cleavage product. The present invention also provides for a kit which includes a SELDI sample chip having surface moieties capable of binding to an enzyme cleavage product.

摘要翻译： 本发明提供了确定酶活性的各种方法。 在一个实施方案中，用于检测酶活性的方法包括使血液成分与包含酶的切割位点和标签部分的底物接触，以产生包含具有已知质量的切割产物的样品，并将样品与 具有结合裂解产物的表面部分的SELDI样品芯片。 在另一个实施方案中，该方法还包括对SELDI样品芯片进行SELDI质谱分析以鉴定切割产物。 本发明还提供了一种试剂盒，其包括具有能够结合酶切割产物的表面部分的SELDI样品芯片。

公开(公告)号：US07893616B2

公开(公告)日：2011-02-22

申请号：US11711946

申请日：2007-02-27

申请人： Haifeng M. Wu ,  Ming Jin ,  Michael G. Bissell ,  Spero R. Cataland

发明人： Haifeng M. Wu ,  Ming Jin ,  Michael G. Bissell ,  Spero R. Cataland

IPC分类号： H01J17/26

CPC分类号： G01N33/6851 ,  C12Q1/37 ,  G01N2333/96486

摘要： The present invention provides for various processes for determining enzyme activity. In one embodiment, a process for detecting an enzyme activity includes contacting a blood component with a substrate comprising a cleavage site of the enzyme and a tag moiety to produce a sample comprising a cleavage product having a known mass, and placing the sample in contact with a SELDI sample chip which has surface moieties which bind to the cleavage product. In another embodiment, the process further includes subjecting the SELDI sample chip to SELDI mass spectrometry to identify the cleavage product. The present invention also provides for a kit which includes a SELDI sample chip having surface moieties capable of binding to an enzyme cleavage product.

摘要翻译： 本发明提供了确定酶活性的各种方法。 在一个实施方案中，用于检测酶活性的方法包括使血液成分与包含酶的切割位点和标签部分的底物接触，以产生包含具有已知质量的切割产物的样品，并将样品与 具有结合于切割产物的表面部分的SELDI样品芯片。 在另一个实施方案中，该方法还包括对SELDI样品芯片进行SELDI质谱分析以鉴定切割产物。 本发明还提供了一种试剂盒，其包括具有能够结合酶切割产物的表面部分的SELDI样品芯片。

公开(公告)号：US20100227776A1

公开(公告)日：2010-09-09

申请号：US12717288

申请日：2010-03-04

申请人： Haifeng M. Wu ,  Shangbin Yang ,  Lihui Xu

发明人： Haifeng M. Wu ,  Shangbin Yang ,  Lihui Xu

IPC分类号： C40B30/10 ,  C12Q1/68 ,  C40B40/00

CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172

摘要： Embodiments include a universal and generic method for rapidly genotyping essentially any single nucleotide polymorphism (SNP) and or other polymorphism. Various embodiments include procedures for SNP and/or allele analysis that are easy, cheap, highly multiplexable, easily automatable, and lend themselves to high-throughput. Embodiments are broadly applicable for all applications where SNP determinations are useful.

摘要翻译： 实施方案包括用于快速基因分型基本上任何单核苷酸多态性（SNP）和/或其他多态性的通用和通用方法。 各种实施方案包括易于，便宜，高度可复用，易于自动化的SNP和/或等位基因分析的程序，并且适合于高通量。 实施例广泛适用于SNP测定有用的所有应用。