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公开(公告)号:US07651839B2
公开(公告)日:2010-01-26
申请号:US11322764
申请日:2005-12-29
申请人: Haikuo Li , Jiehuan Huang , Junhong Lv , Xiaodong Zhang , Jun Hu , Zhizhou Zhang , Chunhai Fan
发明人: Haikuo Li , Jiehuan Huang , Junhong Lv , Xiaodong Zhang , Jun Hu , Zhizhou Zhang , Chunhai Fan
CPC分类号: C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , C12Q1/686 , C12Q2563/137
摘要: The present invention discloses a method for optimizing PCR amplification by adding elementary substance material into PCR system, wherein the elementary substance material is selected from a group consisting of element titanium, element nickel, element bismuth, element stibium, element selenium, element chromium, and a mixture of the group. This new method is more effective than conventional amplifying method and could be widely employed in many fronts, especially in multiplex PCR, two-round PCR, low-copy PCR, long-term PCR and rapid PCR.
摘要翻译: 本发明公开了一种通过将基本物质材料添加到PCR系统中来优化PCR扩增的方法,其中基本物质材料选自元素钛,元素镍,元素铋,元素锑,元素硒,元素铬和 该组的混合物。 这种新方法比常规扩增方法更有效,可广泛应用于许多方面,特别是在多重PCR,双向PCR,低拷贝PCR,长期PCR和快速PCR中。
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公开(公告)号:US20060166240A1
公开(公告)日:2006-07-27
申请号:US11322764
申请日:2005-12-29
申请人: Haikuo Li , Jiehuan Huang , Junhong Lv , Xiaodong Zhang , Jun Hu , Zhizhou Zhang , Chunhai Fan
发明人: Haikuo Li , Jiehuan Huang , Junhong Lv , Xiaodong Zhang , Jun Hu , Zhizhou Zhang , Chunhai Fan
CPC分类号: C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , C12Q1/686 , C12Q2563/137
摘要: The present invention discloses a method for optimizing PCR amplification by adding elementary substance material into PCR system, wherein the elementary substance material is selected from a group consisting of element titanium, element nickel, element bismuth, element stibium, element selenium, element chromium, and a mixture of the group. This new method is more effective than conventional amplifying method and could be widely employed in many fronts, especially in multiplex PCR, two-round PCR, low-copy PCR, long-term PCR and rapid PCR.
摘要翻译: 本发明公开了一种通过将基本物质材料添加到PCR系统中来优化PCR扩增的方法,其中基本物质材料选自元素钛,元素镍,元素铋,元素锑,元素硒,元素铬和 该组的混合物。 这种新方法比常规扩增方法更有效,可广泛应用于许多方面,特别是在多重PCR,双向PCR,低拷贝PCR,长期PCR和快速PCR中。
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