公开(公告)号：US20100003719A1

公开(公告)日：2010-01-07

申请号：US12405003

申请日：2009-03-16

申请人： Hans Christian Thagersen ,  Michael Etzerodt ,  Thor Las Holtet ,  Niels Jonas Heilskov Graversen ,  Jette Sandholm Kastrup ,  Bettina Bryde Nielsen ,  Ingrid Kjoller Larsen

发明人： Hans Christian Thagersen ,  Michael Etzerodt ,  Thor Las Holtet ,  Niels Jonas Heilskov Graversen ,  Jette Sandholm Kastrup ,  Bettina Bryde Nielsen ,  Ingrid Kjoller Larsen

IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N5/00 ,  C12N1/00 ,  C12N15/63

CPC分类号： C12N15/62 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4726 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/41 ,  C07K2319/50 ,  C07K2319/73 ,  C07K2319/95

摘要： The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.