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公开(公告)号：US20050015829A1
公开(公告)日：2005-01-20
申请号：US10482549
申请日：2002-04-30
申请人： Hans-Ulrich Koop , Stefan Muhlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy Golds
发明人： Hans-Ulrich Koop , Stefan Muhlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy Golds
CPC分类号： C12N15/8212 , C12N15/821 , C12N15/8214 , C12N15/8257
摘要： A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.
摘要翻译： 一种产生转化的植物或植物细胞的方法，所述转基因植物或植物细胞在其塑料体上转化并且没有选择标记，包括以下步骤：（a）用DNA转化植物或植物细胞的质体，所述DNA包括：（i）赋予复制的核苷酸序列 所述DNA在植物细胞中，（ii）至少一种感兴趣的序列，（iii）侧翼于所述至少一个感兴趣序列侧翼的序列，所述至少一个感兴趣序列用于将所述至少一个感兴趣的序列稳定整合到质体基因组中，以及（iv ）在所述感兴趣的序列侧翼的所述序列之外的选择标记; （b）允许在存在选择压力的情况下将所述至少一个感兴趣序列整合到塑料瓶中; （c）通过释放选择压力允许所述选择标记序列的丢失; 和（d）回收在其塑料上遗传转化并且没有所述选择标记的细胞和/或植物。
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公开(公告)号：US20070016983A1
公开(公告)日：2007-01-18
申请号：US10581703
申请日：2004-12-03
申请人： Stefan Muhlbauer
发明人： Stefan Muhlbauer
CPC分类号： C12N15/8214 , C12N15/8238
摘要： A process of controlling expression of a plastome-encoded sequence of interest in a plant or in plant cells by externally applying to said plant or to said plant cells a chemical or physical control signal, wherein said control signal is adapted for interacting with an intra-plastid component of the plastid protein expression machinery and wherein expression of said sequence of interest is controlled by said control signal.
摘要翻译： 通过向所述植物或所述植物细胞外部施用化学或物理控制信号来控制植物或植物细胞中感兴趣的质体组编码序列的表达的方法，其中所述控制信号适于与植物细胞内相互作用， 质体蛋白质表达机制的质体组分，并且其中所述感兴趣序列的表达由所述控制信号控制。
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公开(公告)号：US20080057563A1
公开(公告)日：2008-03-06
申请号：US11631719
申请日：2005-07-07
申请人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
发明人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译： 通过从植物或植物叶中感兴趣的序列表达所述目标蛋白质来产生感兴趣的蛋白质的方法，包括：（a）通过用所述植物或所述植物叶子渗透所述植物或所述植物叶来转染所述植物或所述植物叶 农杆菌菌株在补充因子存在下，所述含有T-DNA的土壤杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中编码复制子的所述序列含有所述复制子的复制子功能所必需的序列，所述序列得自 （b）从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述感兴趣的蛋白质，其中所述农杆菌菌株被提供有第一种遗传修饰物 使得所述农杆菌菌株在缺乏所述补体因子的情况下缺陷以用所述T-DNA转染生物体。
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