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公开(公告)号：US20080057563A1
公开(公告)日：2008-03-06
申请号：US11631719
申请日：2005-07-07
申请人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
发明人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译： 通过从植物或植物叶中感兴趣的序列表达所述目标蛋白质来产生感兴趣的蛋白质的方法，包括：（a）通过用所述植物或所述植物叶子渗透所述植物或所述植物叶来转染所述植物或所述植物叶 农杆菌菌株在补充因子存在下，所述含有T-DNA的土壤杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中编码复制子的所述序列含有所述复制子的复制子功能所必需的序列，所述序列得自 （b）从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述感兴趣的蛋白质，其中所述农杆菌菌株被提供有第一种遗传修饰物 使得所述农杆菌菌株在缺乏所述补体因子的情况下缺陷以用所述T-DNA转染生物体。
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公开(公告)号：US08093458B2
公开(公告)日：2012-01-10
申请号：US11631719
申请日：2005-07-07
申请人： Sylvestre Marillonnet , Carola Engler , Stefan Mühlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
发明人： Sylvestre Marillonnet , Carola Engler , Stefan Mühlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译： 通过从植物或植物叶中感兴趣的序列表达所述目标蛋白质来产生感兴趣的蛋白质的方法，包括：（a）通过用所述植物或所述植物叶子渗透所述植物或所述植物叶来转染所述植物或所述植物叶 农杆菌菌株在补充因子存在下，所述含有T-DNA的土壤杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中编码复制子的所述序列含有所述复制子的复制子功能所必需的序列，所述序列得自 从所述复制子表达的所述感兴趣的序列，（b）任选地从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供有第一遗传 在没有所述补充因子的情况下，使所述农杆菌菌株对所述T-DNA转染生物有缺陷。
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公开(公告)号：US07528292B2
公开(公告)日：2009-05-05
申请号：US10523474
申请日：2003-08-01
CPC分类号： C12N15/8214
摘要： The invention provides processes for generating transgenic plants or plant cells transformed on their plastome, comprising introducing into plant plastids a first and a second DNA molecule, wherein the first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and the second DNA molecule contains a second region homologous to a region of the plastome and a second sequence of interest, whereby a sequence segment of the first sequence of interest is homologous to a sequence segment of the second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby the integration sequence contains at least a portion of the first and second sequences of interest as a continuous sequence. The invention further provides plant cells, plants, and seeds of plants produced by such processes.
摘要翻译： 本发明提供用于产生在其塑料体上转化的转基因植物或植物细胞的方法，包括向植物质体中引入第一和第二DNA分子，其中所述第一DNA分子包含与所述塑性体的区域同源的第一区域，用于引导塑性体整合， 第一个感兴趣的序列，并且第二个DNA分子包含与Plastome的区域同源的第二个区域和第二个感兴趣的序列，其中第一个目的序列的序列片段与第二个序列的序列片段同源 兴趣，并选择具有稳定整合在塑性体中的整合序列的转化体，由此整合序列包含作为连续序列的第一和第二感兴趣序列的至少一部分。 本发明还提供通过这些方法生产的植物的植物细胞，植物和种子。
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公开(公告)号：US07193131B2
公开(公告)日：2007-03-20
申请号：US10466221
申请日：2002-01-18
申请人： Christian Eibl , Fong-Chin Huang , Sebastian Klaus , Stefan Mühlbauer , Stefan Herz , Hans-Ulrich Koop
发明人： Christian Eibl , Fong-Chin Huang , Sebastian Klaus , Stefan Mühlbauer , Stefan Herz , Hans-Ulrich Koop
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8209
摘要： A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.
摘要翻译： 提供了通过以下步骤生产多细胞植物，植物器官或其植物组织转化的方法：（a）改变或破坏质体基因组中的基因的功能以产生可选择或可识别的表型; （b）分离或选择具有表达所述表型的质体的植物或细胞; （c）用具有能够恢复所述功能的恢复序列的至少一个转化载体转化所述分离或选择的植物，植物器官或植物组织的所述质体基因组; 和（d）分离或选择具有表达所述恢复功能的质体的所述转化植物，植物器官或植物组织。
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公开(公告)号：US07534742B2
公开(公告)日：2009-05-19
申请号：US11526318
申请日：2006-09-25
申请人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Lüttgen , Felix Rohdich , Silvia Sagner , Christoph A. Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart H. Zenk
发明人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Lüttgen , Felix Rohdich , Silvia Sagner , Christoph A. Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart H. Zenk
IPC分类号： C40B30/04
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibitors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.
摘要翻译： 本发明涉及涉及类异戊二烯生物合成的酶活性以及在类异戊二烯的生物合成中用于酶的抑制剂，特别是除草剂。 更具体地，本发明涉及用于检测这些抑制剂的筛选方法和用于进行所述方法的酶活性蛋白质以及编码这种蛋白质的纯化的分离的DNA。 此外，本发明涉及可通过所述筛选方法检测的新型抑制剂，以及用于抑制类异戊二烯的合成和基于所述抑制剂控制生物体的生长的组合物和方法。 本发明还涉及抑制剂抗性植物酶和植物，植物组织，植物种子和植物细胞的发展。
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公开(公告)号：US20070015216A1
公开(公告)日：2007-01-18
申请号：US11526318
申请日：2006-09-25
申请人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Luttgen , Felix Rohdich , Silvia Sagner-Grehn , Christoph Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart Zenk
发明人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Luttgen , Felix Rohdich , Silvia Sagner-Grehn , Christoph Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart Zenk
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibitors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.
摘要翻译： 本发明涉及涉及类异戊二烯生物合成的酶活性以及在类异戊二烯的生物合成中用于酶的抑制剂，特别是除草剂。 更具体地，本发明涉及用于检测这些抑制剂的筛选方法和用于进行所述方法的酶活性蛋白质以及编码这种蛋白质的纯化的分离的DNA。 此外，本发明涉及可通过所述筛选方法检测的新型抑制剂，以及用于抑制类异戊二烯的合成和基于所述抑制剂控制生物体的生长的组合物和方法。 本发明还涉及抑制剂抗性植物酶和植物，植物组织，植物种子和植物细胞的发展。
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公开(公告)号：US07122331B1
公开(公告)日：2006-10-17
申请号：US10048874
申请日：2000-08-03
申请人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Lüttgen , Felix Rohdich , Silvia Sagner-Grehn , Christoph A. Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart H. Zenk
发明人： Wolfgang Eisenreich , Monika Fellermeier , Markus Fischer , Stefan Hecht , Stefan Herz , Klaus Kis , Holger Lüttgen , Felix Rohdich , Silvia Sagner-Grehn , Christoph A. Schuhr , Jurathip Wungsintaweekul , Adelbert Bacher , Meinhart H. Zenk
IPC分类号： G01N33/573
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibiors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.
摘要翻译： 本发明涉及涉及类异戊二烯生物合成的酶活性以及在类异戊二烯的生物合成中用于酶的抑制剂，特别是除草剂。 更具体地，本发明涉及用于检测这些抑制剂的筛选方法和用于进行所述方法的酶活性蛋白质以及编码这种蛋白质的纯化的分离的DNA。 此外，本发明涉及可通过所述筛选方法检测的新型抑制剂以及用于抑制类异戊二烯的合成和基于所述抑制剂控制生物体的生长的组合物和方法。 本发明还涉及抑制剂抗性植物酶和植物，植物组织，植物种子和植物细胞的发展。
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公开(公告)号：US20060117400A1
公开(公告)日：2006-06-01
申请号：US10523474
申请日：2003-08-01
申请人： Stefan Herz , Hans Koop , Timothy Golds , Christian Eibl
发明人： Stefan Herz , Hans Koop , Timothy Golds , Christian Eibl
CPC分类号： C12N15/8214
摘要： A process of generating transgenic plants or plant cells transformed on their plastome, comprising (a) introducing into plant plastids a first DNA molecule and a second DNA molecule, wherein said first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and said second DNA molecule contains a second region homologous to a region of the plastome for directing plastome integration and a second sequence of interest, whereby a sequence segment of said first sequence of interest is homologous to a sequence segment of said second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby said integration sequence contains at least a portion of said first and at least a portion of said second sequence of interest as a continuous sequence.
摘要翻译： 一种产生在其塑料体上转化的转基因植物或植物细胞的方法，包括（a）向植物质体中引入第一DNA分子和第二DNA分子，其中所述第一DNA分子包含与所述塑性体的区域同源的第一区域，用于引导 plastome整合和第一个兴趣序列，并且所述第二DNA分子含有与所述plastome区域同源的第二区域，用于引导Plastome整合和第二感兴趣的序列，其中所述第一感兴趣序列的序列片段与 所述第二感兴趣序列的序列片段，以及选择具有稳定整合在所述塑性体中的整合序列的转化体，由此所述整合序列包含所述第一和至少一部分所述第二感兴趣序列的连续序列的至少一部分。
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