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    Methods of using cleavable solid phases for isolating nucleic acids
    1.
    发明申请
    Methods of using cleavable solid phases for isolating nucleic acids 审中-公开
    使用可切割固相分离核酸的方法

    公开(公告)号：US20050106576A1

    公开(公告)日：2005-05-19

    申请号：US10714763

    申请日：2003-11-17

    申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

    发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C07H21/04 ,  C07B2200/11

    摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

    摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或季铵基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。