-
公开(公告)号：US20100267071A1
公开(公告)日：2010-10-21
申请号：US12714346
申请日：2010-02-26
申请人： Hashem AKHAVAN-TAFTI , Dean G. BINGER , Ying Chen , Renuka DE SILVA , Terri MCLERNON , James MENDOZA , Bruce H. ODEGAARD , Michael SALVATI , Nir SHAPIR , Wenhua XIE
发明人： Hashem AKHAVAN-TAFTI , Dean G. BINGER , Ying Chen , Renuka DE SILVA , Terri MCLERNON , James MENDOZA , Bruce H. ODEGAARD , Michael SALVATI , Nir SHAPIR , Wenhua XIE
CPC分类号： G01N21/76 , C09B15/00 , C12Q1/28 , G01N21/763 , G01N33/542 , G01N33/582
摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
摘要翻译： 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。
-
公开(公告)号：US07560556B2
公开(公告)日：2009-07-14
申请号：US11072457
申请日：2005-03-04
IPC分类号： C07D221/06 , G01N33/535 , C12Q1/28
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C07D409/04 , C07D409/06 , C07D411/04 , C07D411/06 , C07F9/64 , C07F9/65586 , C12Q1/28 , G01N33/581 , G01N2333/908
摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。
-
公开(公告)号：US07247726B2
公开(公告)日：2007-07-24
申请号：US10468257
申请日：2002-12-18
IPC分类号： C02F9/06 , C07D219/02
CPC分类号： C07D409/04 , C07D219/02 , C07D219/04 , C07D219/06 , C07D411/04 , C07F9/64 , C12Q1/28 , G01N33/535 , Y10S435/968
摘要： Novel compounds comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence rapidly by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了包含在一个碳上被两个含硫原子的基团取代的C-C双键的新型化合物。 该化合物可用于通过与过氧化物酶和过氧化物的反应快速产生化学发光的方法和组合物。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。
-
公开(公告)号：US06897036B2
公开(公告)日：2005-05-24
申请号：US10163750
申请日：2002-06-06
IPC分类号： C12Q1/28 , G01N33/533 , C12Q1/00 , G01N33/53
CPC分类号： C12Q1/28 , G01N33/533 , Y10S435/968
摘要： Methods of producing fluorescence from fluorogenic substrates reactive with a peroxidase enzyme are disclosed. Use of the methods in assays for peroxidase enzymes, peroxidase-labeled analytes are provided. Fluorogenic compounds, compositions and kits for reaction with peroxidase enzymes are described. Two modes of producing fluorescent compounds are described.
摘要翻译： 公开了从与过氧化物酶反应的荧光底物产生荧光的方法。 提供了在过氧化物酶，过氧化物酶标记的分析物的测定中使用该方法。 描述了与过氧化物酶反应的荧光化合物，组合物和试剂盒。 描述了产生荧光化合物的两种模式。
-
5.发明授权
公开(公告)号：US5631167A
公开(公告)日：1997-05-20
申请号：US509692
申请日：1995-07-31
IPC分类号： G01N21/78 , C07D321/00 , C07F9/54 , C09K11/06 , C09K11/07 , C12Q1/42 , G01N33/53 , G01N33/58 , G01N35/08 , G01N21/76
CPC分类号： G01N33/582 , G01N33/53 , Y10T436/118339
摘要： Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light.The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.
摘要翻译： 提供了利用稳定的，酶促触发的具有改善的水溶性的化学发光1,2-二氧杂环丁烷的改进的测定方法。 测定通过胶囊化学分析测定方法进行，其中包含分离的水性片段的流体胶囊包含化学发光二氧杂环丁烷试剂或由油基分离流体分离的活化剂，流过导管，随后反应产生光。 改进之处在于使用由二环氧乙烷结构上设置的两个或更多个水溶性基团取代的二氧杂环丁烷以提供化学发光。 包含这种二氧杂环丁烷，非聚合物表面活性剂增强剂和任选的荧光剂的组合物在用于胶囊化学分析系统的测定中使用时，提供增强的化学发光并消除试剂残留的问题。
-
公开(公告)号：US08106203B2
公开(公告)日：2012-01-31
申请号：US12476668
申请日：2009-06-02
IPC分类号： C07D219/00 , C12Q1/28
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C07D409/04 , C07D409/06 , C07D411/04 , C07D411/06 , C07F9/64 , C07F9/65586 , C12Q1/28 , G01N33/581 , G01N2333/908
摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。
-
公开(公告)号：US20070190526A1
公开(公告)日：2007-08-16
申请号：US11706547
申请日：2007-02-15
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
CPC分类号： C12Q1/6806
摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。
-
公开(公告)号：US20050106576A1
公开(公告)日：2005-05-19
申请号：US10714763
申请日：2003-11-17
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Nicole Cilli , William Cripps , Richard Handley , Elizabeth O'Connor , Lekkala Reddy , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Nicole Cilli , William Cripps , Richard Handley , Elizabeth O'Connor , Lekkala Reddy , Sarada Siripurapu
CPC分类号： C07H21/04 , C07B2200/11
摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.
摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或季铵基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。
-
9.发明授权Di-substituted 1,2-dioxetane compounds having increased water solubility and assay compositions 失效具有增加的水溶性和测定组成的二取代的1,2-二氧杂环丁烷化合物
公开(公告)号：US5777135A
公开(公告)日：1998-07-07
申请号：US509305
申请日：1995-07-31
IPC分类号： G01N33/535 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N33/53 , G01N33/543 , G01N33/58 , C07C69/76 , C07F9/02
CPC分类号： G01N33/582 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , C07C2103/00
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Dioxetanes further instituted with two or more water-solubilizing groups disposed on the dioxetane structure provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. Compositions comprising a dioxetane with two or more water-solubilizing groups, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： 提供了具有改善的水溶性的稳定的酶触发的化学发光的1,2-二氧杂环丁烷。 二氧杂环丁烷结构上设置的两个或多个水溶性基团进一步制备的二氧杂环丁烷，当用于胶囊化学分析系统的分析中时，通过消除试剂残留问题，可提供优异的性能。 还提供了包含二环氧乙烷与两个或多个水溶性基团的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
-
公开(公告)号：US09029092B2
公开(公告)日：2015-05-12
申请号：US12714346
申请日：2010-02-26
申请人： Hashem Akhavan-Tafti , Dean G. Binger , Ying Chen , Renuka De Silva , Terri McLernon , James Mendoza , Bruce H. Odegaard , Michael Salvati , Nir Shapir , Wenhua Xie
发明人： Hashem Akhavan-Tafti , Dean G. Binger , Ying Chen , Renuka De Silva , Terri McLernon , James Mendoza , Bruce H. Odegaard , Michael Salvati , Nir Shapir , Wenhua Xie
IPC分类号： G01N33/53 , G01N30/00 , C12Q1/28 , G01N33/542 , G01N33/58
CPC分类号： G01N21/76 , C09B15/00 , C12Q1/28 , G01N21/763 , G01N33/542 , G01N33/582
摘要： Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
摘要翻译： 公开了用于确定疑似含有分析物的样品中的分析物的方法，试剂，试剂盒和系统，其中所有试剂都可溶于水溶液。 一种测定方法包括在如下条件下处理怀疑含有分析物的样品，使得如果存在分析物，则使活化剂与化学发光化合物反应构型以使其活化。 样品也用试剂处理以减少与分析物无关的信号。 最后，用触发溶液处理样品，从而产生来自活化化学发光化合物的光。 没有试剂与表面或其他固相相关。
-
-
-
-
-
-
-
-
-
搜索结果
22 条记录 (耗时 0.022 秒)
