公开(公告)号：US20050106589A1

公开(公告)日：2005-05-19

申请号：US10891880

申请日：2004-07-15

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Sarada Siripurapu ,  Richard Handley

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Sarada Siripurapu ,  Richard Handley

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/101 ,  C12Q1/6823

摘要： Methods of isolating nucleic acids are disclosed comprising binding the nucleic acid to solid phase binding materials and releasing the bound nucleic acid from the solid phase by elution with a novel reagent composition. Compositions feature a high ionic strength buffer or an added hydrophilic organic co-solvent or both. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.

摘要翻译： 公开了分离核酸的方法，其包括将核酸与固相结合材料结合，并通过用新的试剂组合物洗脱从固相中释放结合的核酸。 组合物具有高离子强度缓冲液或添加的亲水性有机共溶剂或两者。 与本发明的方法和组合物一起使用的优选固相材料包含季鎓核酸结合部分。

公开(公告)号：US20070190526A1

公开(公告)日：2007-08-16

申请号：US11706547

申请日：2007-02-15

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Robert A. Eickholt ,  Michael E. Mazelis ,  Wenhuas Xie ,  Richard S. Handley ,  Monica A. Bray ,  Michelle L. Mastronardi ,  Elizabeth A. O'Conner ,  Sarada Siripurapu

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Robert A. Eickholt ,  Michael E. Mazelis ,  Wenhuas Xie ,  Richard S. Handley ,  Monica A. Bray ,  Michelle L. Mastronardi ,  Elizabeth A. O'Conner ,  Sarada Siripurapu

IPC分类号： C12Q1/70 ,  C07H21/02

CPC分类号： C12Q1/6806

摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.

摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。

公开(公告)号：US20050106576A1

公开(公告)日：2005-05-19

申请号：US10714763

申请日：2003-11-17

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Nicole Cilli ,  William Cripps ,  Richard Handley ,  Elizabeth O'Connor ,  Lekkala Reddy ,  Sarada Siripurapu

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C07H21/04 ,  C07B2200/11

摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或季铵基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。