公开(公告)号：US08263415B2

公开(公告)日：2012-09-11

申请号：US12377021

申请日：2007-09-12

申请人： Henrik Berling ,  Tanja Jarhede ,  Anita Larsson ,  Hakan Roos

发明人： Henrik Berling ,  Tanja Jarhede ,  Anita Larsson ,  Hakan Roos

IPC分类号： G01N33/566

CPC分类号： G01N33/54306 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/54366 ,  G01N33/54393

摘要： A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.

摘要翻译： 确定流体样品中分析物的总浓度的方法，其中至少部分分析物与分析物结合物质作为复合物存在。 所述方法包括以下步骤：a）使样品经受降低分析物和分析物结合物质之间的结合亲和力的条件，足以使基本上解析任何分析物复合物并基本上提供游离形式的所有分析物，b）使样品经受 恢复分析物和分析物结合物质之间的结合亲和力，以及c）在结合亲和力恢复后立即，并且在分析物发生任何显着的重新络合之前，确定样品中游离分析物的浓度。 还公开了测定样品中复合物结合分析物浓度的方法。

公开(公告)号：US20100167422A1

公开(公告)日：2010-07-01

申请号：US12377021

申请日：2007-09-12

申请人： Henrik Berling ,  Tanja Jarhede ,  Anita Larsson ,  Hakan Roos

发明人： Henrik Berling ,  Tanja Jarhede ,  Anita Larsson ,  Hakan Roos

IPC分类号： G01N33/543

CPC分类号： G01N33/54306 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/54366 ,  G01N33/54393

摘要： A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.

摘要翻译： 确定流体样品中分析物的总浓度的方法，其中至少部分分析物与分析物结合物质作为复合物存在。 所述方法包括以下步骤：a）使样品经受降低分析物和分析物结合物质之间的结合亲和力的条件，足以使基本上解析任何分析物复合物并基本上提供游离形式的所有分析物，b）使样品经受 恢复分析物和分析物结合物质之间的结合亲和力，以及c）在结合亲和力恢复后立即，并且在分析物发生任何显着的重新络合之前，确定样品中游离分析物的浓度。 还公开了测定样品中复合物结合分析物浓度的方法。

公开(公告)号：US20060094060A1

公开(公告)日：2006-05-04

申请号：US11249038

申请日：2005-10-11

申请人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

发明人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

IPC分类号： G01N33/53 ,  B05D3/00

CPC分类号： G01N33/548 ,  B82Y30/00 ,  G01N2800/52 ,  Y10S436/809

摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.

摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。

公开(公告)号：US08093005B2

公开(公告)日：2012-01-10

申请号：US12581431

申请日：2009-10-19

申请人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

发明人： Tanja Jarhede ,  Per Kjellin ,  Anita Larsson ,  Hans Sjobom

IPC分类号： G01N33/53 ,  G01N31/00

CPC分类号： G01N33/548 ,  B82Y30/00 ,  G01N2800/52 ,  Y10S436/809

摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.

摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。