公开(公告)号：US20060110375A1

公开(公告)日：2006-05-25

申请号：US10524187

申请日：2003-08-11

申请人： Herbert Zech ,  Nicolas Zech ,  Gottfried Dohr ,  Pierre Vanderzwalmen

发明人： Herbert Zech ,  Nicolas Zech ,  Gottfried Dohr ,  Pierre Vanderzwalmen

IPC分类号： A61K48/00 ,  C12N5/06

CPC分类号： G07D3/14 ,  C12N15/873

摘要： A method for producing cell lines or individual organs, differentiable donor cells being supplied to a morula or blastocyst which is cultivated under conditions that ensure a further development of the morula or blastocyst in stages in which newly formed cell lines having a higher degree of differentiation occur, as well as isolation of the cell lines or further differentiation of the cell lines into organs. The method according to the present invention is distinguished in that the cells of the morula or the internal cell mass of the blastocyst have a restricted survivability in comparison to the particular wild type or their survivability is reduced through suitable cultivation conditions, and the donor cells supplied to the morula or blastocyst have varying degrees of differentiation. A significantly shortened cultivation period of the donor cells may thus be achieved in comparison to typical methods, through which the provision of natural stem cells is favored, and perhaps made possible for the first time. The blastocysts may also be used by being transferred into a surrogate mother animal.

摘要翻译： 一种生产细胞系或单个器官的方法，将可分化的供体细胞供应到桑ula或胚泡，其在确保新生成的具有较高分化程度的细胞系的阶段中进一步发育桑ula或胚泡的条件下培养 ，以及细胞系的分离或细胞系进一步分化成器官。 根据本发明的方法的特征在于，与特定野生型相比，囊胚的细胞或囊胚的内部细胞团体具有限制的存活性，或者通过合适的培养条件降低它们的存活力，并且供体细胞 对桑ula或囊胚有不同程度的分化。 与典型的方法相比，可以实现供体细胞的显着缩短的培养期，通过这些方法天然干细胞的提供是有利的，并且可能是第一次可能的。 囊胚也可以通过转移到替代母亲动物中使用。