公开(公告)号：US06274359B1

公开(公告)日：2001-08-14

申请号：US09111730

申请日：1998-07-08

申请人： Hideharu Anazawa ,  Hiroko Shimada ,  Seiji Sugimoto ,  Tatsuo Suda ,  Toshimasa Shinki ,  Takao Saruta ,  Yuzuru Ishimura ,  Matsuhiko Hayashi ,  Shu Wakino ,  Toshiaki Monkawa ,  Tadashi Yoshida ,  Hiromichi Suzuki

发明人： Hideharu Anazawa ,  Hiroko Shimada ,  Seiji Sugimoto ,  Tatsuo Suda ,  Toshimasa Shinki ,  Takao Saruta ,  Yuzuru Ishimura ,  Matsuhiko Hayashi ,  Shu Wakino ,  Toshiaki Monkawa ,  Tadashi Yoshida ,  Hiromichi Suzuki

IPC分类号： C12N902

CPC分类号： C12N9/0071 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00

摘要： The present invention relates to a polypeptide having 25-hydroxyvitamin D3-1&agr;-hydroxylase activity, being useful for the prevention, diagnosis and therapeutic treatment of adult diseases such as osteoporosis induced by the decrease of active type vitamin D3 and catalyzing the final stage of vitamin D3 activation; and the gene encoding the polypeptide. In accordance with the present invention, the following can be provided; a polypeptide having 25-hydroxyvitamin D3-1&agr;-hydroxylase activity, DNA encoding the polypeptide, a recombinant DNA prepared by inserting the DNA in a vector, a transformant carrying the recombinant DNA, a method for preparing 25-hydroxyvitamin D3-1&agr;-hydroxylase by using the transformant, a method for preparing 1&agr;, 25-dihydroxyvitamin D3 comprising using the polypeptide having 25-hydroxyvitamin D3-1&agr;-hydroxylase activity, and an antibody recognizing the polypeptide.

摘要翻译： 本发明涉及具有25-羟基维生素D3-1alpha-羟化酶活性的多肽，可用于预防，诊断和治疗成人疾病如由活性型维生素D3的减少引起的骨质疏松症并催化维生素的最终阶段 D3激活; 和编码多肽的基因。根据本发明，可以提供以下： 具有25-羟基维生素D3-1alpha-羟化酶活性的多肽，编码该多肽的DNA，通过将DNA插入载体制备的重组DNA，携带重组DNA的转化体，通过以下方法制备25-羟基维生素D3-1alpha-羟化酶的方法 使用转化体，制备包含使用具有25-羟基维生素D3-1α-羟化酶活性的多肽的1α，25-二羟基维生素D 3的方法和识别该多肽的抗体。

公开(公告)号：US20060173069A1

公开(公告)日：2006-08-03

申请号：US10564367

申请日：2004-07-15

申请人： Hiroyuki Osada ,  Kiyotaka Machida ,  Takeshi Shimizu ,  Tadashi Nakata ,  Toshimasa Shinki ,  Je-Tae Woo ,  Kazuo Nagai

发明人： Hiroyuki Osada ,  Kiyotaka Machida ,  Takeshi Shimizu ,  Tadashi Nakata ,  Toshimasa Shinki ,  Je-Tae Woo ,  Kazuo Nagai

IPC分类号： A61K31/695 ,  A61K31/353 ,  C07F7/02

CPC分类号： C07D493/10 ,  Y02P20/55

摘要： The present invention has an object to provide a novel therapeutic agent for hypercalcemia and bone diseases, and a novel compound capable of being used as an active ingredient of the therapeutic agent. The present invention is a therapeutic agent for hypercalcemia or bone diseases, comprising as an active ingredient a reveromycin A derivative represented by the general formula (I) or a pharmaceutically acceptable salt thereof. (R represents a hydroxyl-protecting group releasable under acidic conditions.)

摘要翻译： 本发明的目的是提供一种用于高钙血症和骨疾病的新型治疗剂，以及能够用作治疗剂的活性成分的新型化合物。 本发明是高钙血症或骨疾病的治疗剂，其含有作为活性成分的由通式（I）表示的复方霉素A衍生物或其药学上可接受的盐。 （R表示在酸性条件下可释放的羟基保护基）