公开(公告)号：US20060173069A1

公开(公告)日：2006-08-03

申请号：US10564367

申请日：2004-07-15

申请人： Hiroyuki Osada ,  Kiyotaka Machida ,  Takeshi Shimizu ,  Tadashi Nakata ,  Toshimasa Shinki ,  Je-Tae Woo ,  Kazuo Nagai

发明人： Hiroyuki Osada ,  Kiyotaka Machida ,  Takeshi Shimizu ,  Tadashi Nakata ,  Toshimasa Shinki ,  Je-Tae Woo ,  Kazuo Nagai

IPC分类号： A61K31/695 ,  A61K31/353 ,  C07F7/02

CPC分类号： C07D493/10 ,  Y02P20/55

摘要： The present invention has an object to provide a novel therapeutic agent for hypercalcemia and bone diseases, and a novel compound capable of being used as an active ingredient of the therapeutic agent. The present invention is a therapeutic agent for hypercalcemia or bone diseases, comprising as an active ingredient a reveromycin A derivative represented by the general formula (I) or a pharmaceutically acceptable salt thereof. (R represents a hydroxyl-protecting group releasable under acidic conditions.)

摘要翻译： 本发明的目的是提供一种用于高钙血症和骨疾病的新型治疗剂，以及能够用作治疗剂的活性成分的新型化合物。 本发明是高钙血症或骨疾病的治疗剂，其含有作为活性成分的由通式（I）表示的复方霉素A衍生物或其药学上可接受的盐。 （R表示在酸性条件下可释放的羟基保护基）

公开(公告)号：US09316588B2

公开(公告)日：2016-04-19

申请号：US13997489

申请日：2011-12-22

申请人： Naohiro Araki ,  Mitsuru Iida ,  Kiyotaka Machida

发明人： Naohiro Araki ,  Mitsuru Iida ,  Kiyotaka Machida

IPC分类号： G01N21/64 ,  G01N33/68

CPC分类号： G01N21/6486 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10 ,  G01N2800/042

摘要： Provided are a procedurally simple and safe method and kit for measuring the concentration in a biological sample of a physiologically active substance which binds to a receptor that causes a change in intracellular cAMP concentration. By providing a composition including genetically-modified cells which show co-expression of a receptor that causes a change in intracellular cAMP concentration, a cyclic nucleotide-responsive calcium channel, and aequorin luminescent protein, it is possible to measure how much of a physiologically active substance which binds to said receptor is included in a biological sample.

摘要翻译： 提供了一种程序上简单和安全的方法和试剂盒，用于测量与引起细胞内cAMP浓度变化的受体结合的生理活性物质的生物样品中的浓度。 通过提供包含遗传修饰的细胞的组合物，其显示引起细胞内cAMP浓度变化的受体的共表达，环核苷酸响应性钙通道和水母发光蛋白发光蛋白质，可以测量多少生理活性 结合所述受体的物质包括在生物样品中。

公开(公告)号：US20130280734A1

公开(公告)日：2013-10-24

申请号：US13997489

申请日：2011-12-22

申请人： Naohiro Araki ,  Mitsuru Iida ,  Kiyotaka Machida

发明人： Naohiro Araki ,  Mitsuru Iida ,  Kiyotaka Machida

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10 ,  G01N2800/042

摘要： Provided are a procedurally simple and safe method and kit for measuring the concentration in a biological sample of a physiologically active substance which binds to a receptor that causes a change in intracellular cAMP concentration. By providing a composition including genetically-modified cells which show co-expression of a receptor that causes a change in intracellular cAMP concentration, a cyclic nucleotide-responsive calcium channel, and aequorin luminescent protein, it is possible to measure how much of a physiologically active substance which binds to said receptor is included in a biological sample.

摘要翻译： 提供了一种程序上简单和安全的方法和试剂盒，用于测量与引起细胞内cAMP浓度变化的受体结合的生理活性物质的生物样品中的浓度。 通过提供包含遗传修饰的细胞的组合物，其显示引起细胞内cAMP浓度变化的受体的共表达，环核苷酸响应性钙通道和水母发光蛋白发光蛋白质，可以测量多少生理活性 结合所述受体的物质包括在生物样品中。