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    BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY
    2.
    发明申请
    BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY 审中-公开
    具有热稳定性的BILIRUBIN氧化酶突变体

    公开(公告)号：US20090117638A1

    公开(公告)日：2009-05-07

    申请号：US11946543

    申请日：2007-11-28

    申请人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    发明人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    IPC分类号： C12N9/02

    CPC分类号： C12N9/001 ,  C12Y103/03005

    摘要： A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, Myrothecium verrucaria.

    摘要翻译： 公开了一种耐热性胆红素氧化酶突变体。 通过SEQ ID NO：1的野生型氨基酸序列的至少一个氨基酸残基的缺失，置换，添加或插入获得胆红素氧化酶。 ID。 来自不完美丝状真菌的红霉素氧化酶的1号。

    FUEL CELL AND ELECTRONIC APPARATUS
    3.
    发明申请
    FUEL CELL AND ELECTRONIC APPARATUS 审中-公开
    燃料电池和电子设备

    公开(公告)号：US20110059374A1

    公开(公告)日：2011-03-10

    申请号：US12921868

    申请日：2009-03-02

    申请人： Hideyuki Kumita ,  Yoshio Goto ,  Takaaki Nakagawa ,  Hideki Sakai ,  Hiroki Mita ,  Ryuhei Matsumoto ,  Yuichi Tokita

    发明人： Hideyuki Kumita ,  Yoshio Goto ,  Takaaki Nakagawa ,  Hideki Sakai ,  Hiroki Mita ,  Ryuhei Matsumoto ,  Yuichi Tokita

    IPC分类号： H01M8/16

    CPC分类号： H01M8/16 ,  Y02E60/527

    摘要： A fuel cell having excellent properties is provided in which, when an enzyme is immobilized in at least one of a positive electrode and a negative electrode, a sufficient buffering capability can be provided even in a high power output operation and the inherent capability of the enzyme can be sufficiently exerted. In a biofuel cell including a structure in which a positive electrode 2 and a negative electrode 1 face each other with an electrolyte layer 3 therebetween, the electrolyte layer 3 containing a buffer material, and the biofuel cell including an enzyme immobilized in at least one of the positive electrode 2 and the negative electrode 1, the electrolyte layer 3 contains a compound including an imidazole ring as a buffer material and at least one acid selected from the group consisting of acetic acid, phosphoric acid, and sulfuric acid is further added to the electrolyte layer 3. As the compound including an imidazole ring, imidazole, 1-methylimidazole, 2-methylimidazole, 4-methylimidazole, 2-ethylimidazole, or the like is used.

    摘要翻译： 提供一种具有优异特性的燃料电池，其中当酶固定在正电极和负电极中的至少一个中时，即使在高功率输出操作和酶的固有能力下也可以提供足够的缓冲能力 可以充分发挥。 在包括其间具有电解质层3的正极2和负极1彼此面对的结构的生物燃料电池中，包含缓冲材料的电解质层3和包含固定在以下的至少一个中的酶的生物燃料电池 正极2和负极1，电解质层3含有包含咪唑环作为缓冲材料的化合物，并且还将至少一种选自乙酸，磷酸和硫酸的酸加入到 作为包含咪唑环的化合物，可以使用咪唑，1-甲基咪唑，2-甲基咪唑，4-甲基咪唑，2-乙基咪唑等。

    FUEL CELL, ELECTRONIC DEVICE, AND BUFFER SOLUTION FOR FUEL CELL
    4.
    发明申请
    FUEL CELL, ELECTRONIC DEVICE, AND BUFFER SOLUTION FOR FUEL CELL 审中-公开
    燃料电池，电子设备和燃料电池的缓冲溶液

    公开(公告)号：US20110200889A1

    公开(公告)日：2011-08-18

    申请号：US13121992

    申请日：2009-10-07

    申请人： Hideyuki Kumita ,  Hiroki Mita ,  Yoshio Goto ,  Takaaki Nakagawa ,  Hideki Sakai ,  Ryuhei Matsumoto ,  Yuichi Tokita

    发明人： Hideyuki Kumita ,  Hiroki Mita ,  Yoshio Goto ,  Takaaki Nakagawa ,  Hideki Sakai ,  Ryuhei Matsumoto ,  Yuichi Tokita

    IPC分类号： H01M8/16 ,  H01M8/08

    CPC分类号： H01M8/16 ,  Y02E60/527

    摘要： A fuel cell with which in the case where an enzyme is immobilized to at least one of a cathode and an anode, sufficient buffer ability is able to be obtained even at the time of high output operation, ability inherent in the enzyme is able to be sufficiently demonstrated, and which has superior performance is provided. In a bio-fuel cell which has a structure in which a cathode and an anode are opposed to each other with an electrolyte layer containing a buffer substance in between, and in which an enzyme is immobilized to at least one of the cathode and the anode, a compound containing an imidazole ring is contained in the electrolyte layer as a buffer substance, and one or more acids selected from the group consisting of acetic acid, phosphoric acid, and sulfuric acid are further added.

    摘要翻译： 在将酶固定在阴极和阳极中的至少一个的情况下的燃料电池，即使在高输出操作时也能获得足够的缓冲能力，酶的固有能力可以是 充分证明，并且具有优异的性能。 在具有其中阴极和阳极彼此相对的结构的生物燃料电池中，其间包含缓冲物质的电解质层，并且其中酶固定在阴极和阳极中的至少一个上 ，作为缓冲物质的电解质层含有含有咪唑环的化合物，进一步添加一种或多种选自乙酸，磷酸和硫酸的酸。

    BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY
    5.
    发明申请
    BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY 失效
    具有热稳定性的BILIRUBIN氧化酶突变体

    公开(公告)号：US20100129891A1

    公开(公告)日：2010-05-27

    申请号：US12468643

    申请日：2009-05-19

    申请人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    发明人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    IPC分类号： C12N9/02

    CPC分类号： C12N9/001 ,  C12Y103/03005

    摘要： A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase mutant is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, Myrothecium verrucaria. A biocathode including a thermo stable bilirubin oxidase mutant immobilized thereon, and its use in a biofuel cell is disclosed.

    摘要翻译： 公开了一种耐热性胆红素氧化酶突变体。 通过SEQ ID NO：1的野生型氨基酸序列的至少一个氨基酸残基的缺失，置换，添加或插入获得胆红素氧化酶突变体。 ID。 来自不完美丝状真菌的红霉素氧化酶的1号。 公开了一种包括固定在其上的热稳定性胆红素氧化酶突变体的生物阴极及其在生物燃料电池中的用途。

    Bilirubin oxidase mutant having thermal stability
    7.
    发明授权
    Bilirubin oxidase mutant having thermal stability 失效
    具有热稳定性的胆红素氧化酶突变体

    公开(公告)号：US08093029B2

    公开(公告)日：2012-01-10

    申请号：US12468643

    申请日：2009-05-19

    申请人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    发明人： Hideyuki Kumita ,  Yuichi Tokita ,  Yoshio Goto

    IPC分类号： C12N9/02 ,  C12N9/00 ,  C12P21/06 ,  C12Q1/00 ,  C12Q1/20 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12N9/001 ,  C12Y103/03005

    摘要： A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase mutant is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, Myrothecium verrucaria. A biocathode including a thermo stable bilirubin oxidase mutant immobilized thereon, and its use in a biofuel cell is disclosed.

    摘要翻译： 公开了一种耐热性胆红素氧化酶突变体。 通过SEQ ID NO：1的野生型氨基酸序列的至少一个氨基酸残基的缺失，置换，添加或插入获得胆红素氧化酶突变体。 ID。 来自不完美丝状真菌的红霉素氧化酶的1号。 公开了一种包括固定在其上的热稳定性胆红素氧化酶突变体的生物阴极及其在生物燃料电池中的用途。

    FUEL REFORMER AND POWER GENERATION APPARATUS USING THE SAME
    9.
    发明申请
    FUEL REFORMER AND POWER GENERATION APPARATUS USING THE SAME 审中-公开
    燃油改造器和使用该燃料的发电装置

    公开(公告)号：US20110117453A1

    公开(公告)日：2011-05-19

    申请号：US13001902

    申请日：2009-06-30

    申请人： Yuichi Tokita ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta ,  Takaaki Nakagawa ,  Hiroki Mita

    发明人： Yuichi Tokita ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta ,  Takaaki Nakagawa ,  Hiroki Mita

    IPC分类号： H01M8/16

    CPC分类号： H01M8/16 ,  H01M8/06 ,  H01M8/0625 ,  Y02E60/527

    摘要： The present invention provides a fuel reformer which enables power generation to be actually performed even in the case of using very-safe familiar things such as food and drink and food scraps as a fuel of a biofuel cell. The fuel reformer is used for a fuel cell which generates power as an oxidation reduction reaction progresses using enzyme as a catalyst, and has: a primary fuel introduction unit for introducing a primary fuel; a fuel reforming unit communicating with the primary fuel introduction unit and reforming the primary fuel to a secondary fuel from which electrons can be emitted by an oxidation reduction reaction using enzyme as a catalyst; and a secondary fuel supplying unit communicating with the fuel reforming unit and supplying the secondary fuel to the fuel cell.

    摘要翻译： 本发明提供一种燃料重整器，其即使在使用非常安全的熟悉的东西如食物和饮料以及作为生物燃料电池的燃料的食物废料的情况下也能够实际发电。 所述燃料重整器用于燃料电池，所述燃料电池随着氧化还原反应而产生电力，所述氧化还原反应使用酶作为催化剂进行，并且具有：用于引入初级燃料的初级燃料引入单元; 燃料重整单元，与主要燃料引入单元连通，并且通过使用酶作为催化剂的氧化还原反应将初级燃料重整成能够从其中排出电子的二次燃料; 以及与燃料重整单元连通并将二次燃料供应到燃料电池的二次燃料供给单元。

    RECOMBINANT BILIRUBIN OXIDASE AND PRODUCTION METHOD FOR THE SAME
    10.
    发明申请
    RECOMBINANT BILIRUBIN OXIDASE AND PRODUCTION METHOD FOR THE SAME 审中-公开
    重组胆红素氧化酶及其生产方法

    公开(公告)号：US20130052714A1

    公开(公告)日：2013-02-28

    申请号：US13643905

    申请日：2011-04-25

    申请人： Hideyuki Kumita ,  Daisuke Yamaguchi ,  Yuichi Tokita ,  Shuji Fujita

    发明人： Hideyuki Kumita ,  Daisuke Yamaguchi ,  Yuichi Tokita ,  Shuji Fujita

    IPC分类号： C12N9/02

    CPC分类号： C12Y103/03005 ,  C12N9/001

    摘要： Disclosed is a method of producing a recombinant bilirubin oxidase, including a step wherein a bilirubin oxidase gene derived from imperfect filamentous fungus Myrothecium verrucaria is introduced to Escherichia coli and expression thereof is allowed. Also disclosed is a recombinant bilirubin oxidase obtained by this production method. This recombinant bilirubin oxidase has an enzymatic activity higher than that of a natural bilirubin oxidase derived from the imperfect filamentous fungus Myrothecium verrucaria.

    摘要翻译： 本发明公开了一种重组胆红素氧化酶的制备方法，其特征在于，将来自不完全丝状真菌疣状疟原虫的胆红素氧化酶基因导入大肠杆菌并进行表达。 还公开了通过该制造方法获得的重组胆红素氧化酶。 这种重组胆红素氧化酶的酶活性高于源自不完美丝状真菌疣状疣虫的天然胆红素氧化酶。