公开(公告)号：US20090101836A1

公开(公告)日：2009-04-23

申请号：US12252669

申请日：2008-10-16

申请人： Hisashi OHTSUKA ,  Kiyoshi Fujimoto

发明人： Hisashi OHTSUKA ,  Kiyoshi Fujimoto

IPC分类号： G01N21/64 ,  B21D33/00

CPC分类号： G01N21/648 ,  G01N21/6428 ,  Y10T29/308

摘要： An excitation light beam is emitted from a light source. The excitation light beam propagates through a substrate and enters an interface between the substrate and a thin metal film having fine apertures with diameters less than or equal to the wavelength of the excitation light beam, provided on the surface of the substrate opposite that on which the excitation light beam is incident. Near field light is generated at the fine apertures. Fluorescent labels, included in a sample which is supplied to contact the thin metal film, are excited by the near field light and/or surface plasmon on the thin metal film induced by the near field light. The fluorescence emitted from the fluorescent labels is detected by a photodetector.

摘要翻译： 激发光束从光源发射。 激发光束通过衬底传播并进入衬底和具有直径小于或等于激发光束的波长的细孔的薄金属膜之间的界面，其设置在衬底的与衬底 激发光束入射。 在细孔处产生近场光。 包含在供应以接触薄金属膜的样品中的荧光标记被由近场光诱导的薄金属膜上的近场光和/或表面等离子体激发。 由荧光标记物发出的荧光由光检测器检测。

公开(公告)号：US20080074671A1

公开(公告)日：2008-03-27

申请号：US11859027

申请日：2007-09-21

申请人： Hisashi OHTSUKA ,  Morihito IKEDA

发明人： Hisashi OHTSUKA ,  Morihito IKEDA

IPC分类号： G01N21/55

CPC分类号： G01N21/648 ,  G01N21/6428

摘要： A fluorescence sensor is constituted by: a light source, for emitting excitation light of a predetermined wavelength; a dielectric block, formed of a material that transmits the excitation light; a metal film, formed on a surface of the dielectric block; a non flexible film of a hydrophobic material, formed on the metal film at a film thickness within a range of 10 to 100 nm; a sample holding portion, for holding a sample such that the sample contacts the non flexible film; an incident optical system, for causing the excitation light to enter the interface between the dielectric block and the metal film through the dielectric block such that conditions for total internal reflection are satisfied; and fluorescence detecting means, for detecting fluorescence emitted by a substance within the sample, which is excited by evanescent waves that leak from the interface when the excitation light enters the interface.

摘要翻译： 荧光传感器由以下部分构成：光源，用于发射预定波长的激发光; 介电块，由透射激发光的材料形成; 金属膜，形成在介质块的表面上; 在10〜100nm的范围内，在金属膜上形成疏水性材料的非柔性膜， 样品保持部分，用于保持样品使得样品接触非柔性膜; 入射光学系统，用于使激发光通过介质块进入介电块和金属膜之间的界面，使得满足全内反射的条件; 以及荧光检测装置，用于检测样品内的物质发出的荧光，当激发光进入界面时，由从界面泄漏的ev逝波激发。

公开(公告)号：US20100006774A1

公开(公告)日：2010-01-14

申请号：US12501906

申请日：2009-07-13

申请人： Hisashi OHTSUKA ,  Kazuyoshi HORII

发明人： Hisashi OHTSUKA ,  Kazuyoshi HORII

IPC分类号： G01N21/64 ,  G01N21/05

CPC分类号： G01N21/648 ,  G01N2021/6439

摘要： A sensor chip including a sensor portion that has at least a metal layer deposited on a surface of a dielectric plate is used. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection target substance in a liquid sample binds onto the sensor-portion. The amount of the detection target substance is detected based on the amount of light generated by excitation of a fluorescent label in the fluorescent-label binding substance. An electrified fluorescent substance containing a plurality of fluorescent dye molecules enclosed by a material that transmits fluorescence output from the plurality of fluorescent dye molecules is used as the fluorescent label. The electrified fluorescent substance is attracted to the sensor portion by applying voltage to the liquid sample in a state in which the fluorescent-label binding substance has bound to the sensor portion. In this state, the amount of the detection target substance is detected.

摘要翻译： 使用包括至少具有沉积在电介质板的表面上的金属层的传感器部分的传感器芯片。 对应于液体样品中的检测对象物质的量的荧光标记结合物质结合到传感器部分上。 基于荧光标记结合物质中的荧光标记物的激发产生的光量，检测出检测对象物质的量。 作为荧光标记，使用含有多个荧光染料分子的带电荧光物质作为荧光标记。 带电的荧光物质通过在荧光标记结合物质与传感器部分结合的状态下向液体样品施加电压而被吸引到传感器部分。 在该状态下，检测出检测对象物质的量。

公开(公告)号：US20090258439A1

公开(公告)日：2009-10-15

申请号：US12422587

申请日：2009-04-13

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N33/558

CPC分类号： G01N33/54386 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808

摘要： An immunochromatoassay method that allows high detection sensitivity measurement. The method including the steps of: permeating an analyte solution that includes a visibly labeled second binding substance that specifically binds to a detection target substance into a test area of a chromatography medium provided with a first binding substance that specifically binds to the detection target substance, simultaneously with or after the permeation of the analyte solution into the test area, permeating a visual recognition aid solution into the chromatograph medium, the solution having a refractive index whose refractive index difference Δn from that of the chromatograph medium is −0.1=Δn=0.1, and visually observing the test area while the visual recognition aid solution is permeated in the test area.

摘要翻译： 允许高检测灵敏度测量的免疫色谱测定方法。 该方法包括以下步骤：将包含特异性结合检测对象物质的可视标记的第二结合物质的分析物溶液渗入具有特异性结合检测对象物质的第一结合物质的色谱介质的检测区域， 在将分析物溶液渗透到测试区域中或之后，将视觉识别助剂溶液渗透到色谱介质中，该折射率与色谱介质的折射率差异Deltan的折射率为-0.1 = Deltan = 0.1 并且在视觉识别辅助溶液渗透到测试区域中的目视观察测试区域。

公开(公告)号：US20080204753A1

公开(公告)日：2008-08-28

申请号：US12038976

申请日：2008-02-28

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N21/64

CPC分类号： G01N21/648 ,  G01N21/6428

摘要： A fluorescence sensor comprises a sensor section for collecting a fluorescent substance, which acts to represent presence of a substance to be detected in a sample, and an exciting light source, which produces exciting light for exciting the fluorescent substance to produce fluorescence. Besides the exciting light source, at least one different non-exciting light source is located for irradiating different non-exciting light, which varies in wavelength from the exciting light and which is substantially free from capability of exciting the fluorescent substance, to the sensor section.

摘要翻译： 荧光传感器包括用于收集用于表示样品中待检测物质的存在的荧光物质的传感器部分和产生用于激发荧光物质以产生荧光的激发光的激发光源。 除了激发光源之外，至少一个不同的非激励光源被定位成用于照射波长与激发光波长不同的非激发光，并且其基本上没有激发荧光物质的能力传递到传感器部分 。

公开(公告)号：US20080179540A1

公开(公告)日：2008-07-31

申请号：US11954565

申请日：2007-12-12

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01J1/58

CPC分类号： G01N21/648 ,  G01N2021/6432

摘要： Exciting light is irradiated through a dielectric material block toward an interface between the dielectric material block and a metal film formed on one surface of the dielectric material block, such that total reflection conditions may be satisfied. A fluorescence detector detects fluorescence produced by a fluorescent substance, which is contained in a sample and produces the fluorescence by being excited by an evanescent wave oozing out from the interface when the exciting light impinges upon the interface. The exciting light has a wavelength causing the fluorescent substance, which is contained in the sample, to undergo multiphoton absorption. The fluorescence detector has sensitivity to a wavelength region of the fluorescence produced by the fluorescent substance through the multiphoton absorption.

摘要翻译： 激发光通过电介质材料块向介电材料块和形成在电介质材料块的一个表面上的金属膜之间的界面照射，使得可以满足全反射条件。 荧光检测器检测由样品中包含的荧光物质产生的荧光，并且当激发光照射在界面上时，通过从界面渗出的瞬逝波激发荧光。 激发光具有导致样品中包含的荧光物质进行多光子吸收的波长。 荧光检测器对由荧光物质通过多光子吸收产生的荧光的波长区域具有灵敏度。

公开(公告)号：US20120322166A1

公开(公告)日：2012-12-20

申请号：US13524865

申请日：2012-06-15

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N21/64

CPC分类号： G01N21/648

摘要： Excitation light from a light source is caused to enter a finely apertured thin metal film, having fine apertures with diameters less than or equal to the wavelength of the excitation light, formed on a surface of a substrate different from a light incident surface thereof, through the substrate, to cause near field light to be generated at the fine apertures. Fluorescent labels included in a sample liquid supplied to contact the thin metal film are caused to emit light by the near field light and by surface plasmon which are induced in the finely apertured thin metal film by the near field light. Fluorescence emitted by the fluorescent labels is detected with a detector. Fluorescent particles having a plurality of fluorescent pigments enveloped in a transmitting material that transmits both the excitation light and the fluorescence are employed as the fluorescent labels.

摘要翻译： 引起来自光源的激发光进入细孔薄金属膜，其具有直径小于或等于激发光的波长的细孔，其形成在不同于其光入射表面的基板的表面上，通过 基板，以在微细孔处产生近场光。 包含在供应用于接触薄金属膜的样品液体中的荧光标记通过近场光和表面等离子体激发使其通过近场光在细孔金属薄膜中诱发。 用检测器检测荧光标记物发出的荧光。 作为荧光标记物，使用具有包含在透射材料中的多个荧光颜料的荧光粒子，其透过激发光和荧光。

公开(公告)号：US20110129942A1

公开(公告)日：2011-06-02

申请号：US12275900

申请日：2008-11-21

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N21/64 ,  G01N33/566

CPC分类号： G01N21/648

摘要： An amount of fluorescent labels corresponding to an amount of a detection target substance are caused to bind on a detecting portion. The fluorescent labels are excited, and the amount of the detection target substance is detected based on the intensity of fluorescence emitted due to the excitation. Fine inorganic fluorescent particles which do not become discolored are employed as the fluorescent labels.

摘要翻译： 使与检测对象物质的量对应的荧光标记物的量结合在检测部上。 荧光标记被激发，并且基于由于激发而发射的荧光的强度来检测检测目标物质的量。 使用未变色的无机荧光微粒作为荧光标记。

公开(公告)号：US20110025315A1

公开(公告)日：2011-02-03

申请号：US12845260

申请日：2010-07-28

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N27/84

CPC分类号： G01N33/54333 ,  B03C1/01 ,  B03C1/28 ,  B03C2201/18 ,  B03C2201/24 ,  B03C2201/26 ,  G01N33/54306

摘要： A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.

摘要翻译： 一种磁性结合物质，其是与靶物质特异性结合的具有磁性包裹电介质粒子的第一结合物质，其中包含磁性粒子，并附着在液体样品中显示极性的官能团修饰的表面，以及 标记结合物质，其是与附着有光响应标记物的目标物质特异性结合的第二结合物质与液体样品混合，从而发生结合反应。 在样品池内产生磁场，将磁性结合物质吸引到局部区域。 激励光仅在包括局部区域的预定区域被照射，同时将磁性物质吸引到局部区域时，使其中存在的光响应标签产生光信号。 检测到光信号。

公开(公告)号：US20090283700A1

公开(公告)日：2009-11-19

申请号：US12465401

申请日：2009-05-13

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01J1/58

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N33/54346 ,  G01N33/54373 ,  G01N33/582

摘要： A fluorescence detecting method utilizes surface plasmon enhancement. An electric field enhancing field is caused to be generated at a detecting portion that includes a metal film provided on a surface of a dielectric prism. Fluorescence emitted by fluorescent labels, which are attached to a detection target substance, due to the excitation effect of the electric field enhancing field is detected by a photodetector. During the detection, a plurality of fine metal particles are dispersed on the detecting portion.

摘要翻译： 荧光检测方法利用表面等离子体增强。 在包括设置在电介质棱镜的表面上的金属膜的检测部分处产生电场增强场。 通过光电检测器检测由于电场增强场的激发效应而附着于检测对象物质的荧光标记发射的荧光。 在检测期间，多个细金属颗粒分散在检测部分上。