公开(公告)号：US08456158B2

公开(公告)日：2013-06-04

申请号：US12845260

申请日：2010-07-28

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N27/84

CPC分类号： G01N33/54333 ,  B03C1/01 ,  B03C1/28 ,  B03C2201/18 ,  B03C2201/24 ,  B03C2201/26 ,  G01N33/54306

摘要： A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.

摘要翻译： 一种磁性结合物质，其是与靶物质特异性结合的具有磁性包裹电介质粒子的第一结合物质，其中包含磁性粒子，并附着在液体样品中显示极性的官能团修饰的表面，以及 标记结合物质，其是与附着有光响应标记物的目标物质特异性结合的第二结合物质与液体样品混合，从而发生结合反应。 在样品池内产生磁场，将磁性结合物质吸引到局部区域。 激励光仅在包括局部区域的预定区域被照射，同时将磁性物质吸引到局部区域时，使其中存在的光响应标签产生光信号。 检测到光信号。

公开(公告)号：US08039268B2

公开(公告)日：2011-10-18

申请号：US12422587

申请日：2009-04-13

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N33/53

CPC分类号： G01N33/54386 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808

摘要： An immunochromatoassay method that allows high detection sensitivity measurement. The method including the steps of: permeating an analyte solution that includes a visibly labeled second binding substance that specifically binds to a detection target substance into a test area of a chromatography medium provided with a first binding substance that specifically binds to the detection target substance, simultaneously with or after the permeation of the analyte solution into the test area, permeating a visual recognition aid solution into the chromatograph medium, the solution having a refractive index whose refractive index difference Δn from that of the chromatograph medium is −0.1≦Δn≦0.1, and visually observing the test area while the visual recognition aid solution is permeated in the test area.

摘要翻译： 允许高检测灵敏度测量的免疫色谱测定方法。 该方法包括以下步骤：将包含特异性结合检测对象物质的可视标记的第二结合物质的分析物溶液渗入具有特异性结合检测对象物质的第一结合物质的色谱介质的检测区域， 在将分析物溶液渗透到测试区域中或之后，将视觉识别助溶剂渗透到色谱介质中，该溶液具有与色谱介质的折射率差＆Dgr; n的折射率为-0.1＆lt; 1lE; ＆Dgr; n＆nlE; 0.1，并且在视觉识别辅助溶液渗入测试区域时目视观察测试区域。

公开(公告)号：US08039267B2

公开(公告)日：2011-10-18

申请号：US12501923

申请日：2009-07-13

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N21/75

CPC分类号： G01N21/6428 ,  G01N21/05 ,  G01N21/648 ,  G01N21/80 ,  G01N33/54373 ,  G01N2021/0346 ,  Y10S436/805

摘要： A sensor-chip includes a sensor-portion having at least a metal-layer deposited on a surface of a dielectric-plate. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection-target-substance in a liquid-sample binds onto the sensor-portion. The amount of the detection-target-substance is detected based on the amount of light generated by excitation of a fluorescent-label in the fluorescent-label binding-substance. A fluorescent-substance the charge state of which changes in the liquid-sample according to the pH of the liquid-sample, and which contains a plurality of fluorescent-dye-molecules enclosed by a material that transmits fluorescence output from the fluorescent-dye-molecules, is used as the fluorescent-label. With the fluorescent-label binding-substance bound to the sensor-portion, the fluorescent-label is attracted to the sensor-portion by adjusting the pH of the sample-liquid to neutralize the charge state of the fluorescent-substance, thereby attracting the fluorescent-label onto the sensor-portion. In this state, the amount of the detection-target-substance is detected.

摘要翻译： 传感器芯片包括具有至少沉积在电介质板的表面上的金属层的传感器部分。 对应于液体样品中的检测对象物质的量的荧光标记结合物质结合到传感器部分上。 基于荧光标记结合物质中的荧光标记物的激发产生的光量来检测检测对象物质的量。 一种荧光物质，其电荷状态根据液体样品的pH而变化，并且其中含有多个荧光染料分子，所述荧光染料分子由透射荧光染料的荧光输出的材料包围， 分子，被用作荧光标记。 通过与传感器部分结合的荧光标记结合物质，通过调节样品液体的pH以中和荧光物质的电荷状态，将荧光标记物吸引到传感器部分，从而吸引荧光标记物 标记到传感器部分上。 在该状态下，检测出检测对象物质的量。

公开(公告)号：US07885769B2

公开(公告)日：2011-02-08

申请号：US11656945

申请日：2007-01-24

申请人： Toshihito Kimura ,  Hisashi Ohtsuka ,  Hiroaki Yamamoto

发明人： Toshihito Kimura ,  Hisashi Ohtsuka ,  Hiroaki Yamamoto

IPC分类号： G01N33/48

CPC分类号： G01N33/54373 ,  B01L3/502 ,  G01N35/00594

摘要： Reference data are subtracted from corresponding actual measurement data, and unprocessed binding quantity data, each of which represents a quantity of binding of a ligand and each of analytes, are thereby acquired. Calculation is made to find a relationship between the reference data and the unprocessed binding quantity data acquired in cases where the binding does not occur. In accordance with the relationship, variation components, which are contained in the unprocessed binding quantity data, are calculated. The variation components are subtracted from the corresponding unprocessed binding quantity data, and processed binding quantity data are thereby acquired. The processed binding quantity data are utilized for extracting an analyte capable of binding with the ligand.

摘要翻译： 从相应的实际测量数据中减去参考数据，从而获得每个表示配体和各分析物的结合量的未处理的结合量数据。 进行计算，以在不发生绑定的情况下找到参考数据与未处理的绑定量数据之间的关系。 根据关系，计算包含在未处理的装订量数据中的变化分量。 从相应的未处理的装订量数据中减去变化分量，从而获得处理的装订量数据。 处理的结合量数据用于提取能够与配体结合的分析物。

公开(公告)号：US07738107B2

公开(公告)日：2010-06-15

申请号：US11859027

申请日：2007-09-21

申请人： Hisashi Ohtsuka ,  Morihito Ikeda

发明人： Hisashi Ohtsuka ,  Morihito Ikeda

IPC分类号： G01N21/55

CPC分类号： G01N21/648 ,  G01N21/6428

摘要： A fluorescence sensor is constituted by: a light source, for emitting excitation light of a predetermined wavelength; a dielectric block, formed of a material that transmits the excitation light; a metal film, formed on a surface of the dielectric block; a non flexible film of a hydrophobic material, formed on the metal film at a film thickness within a range of 10 to 100 nm; a sample holding portion, for holding a sample such that the sample contacts the non flexible film; an incident optical system, for causing the excitation light to enter the interface between the dielectric block and the metal film through the dielectric block such that conditions for total internal reflection are satisfied; and fluorescence detecting means, for detecting fluorescence emitted by a substance within the sample, which is excited by evanescent waves that leak from the interface when the excitation light enters the interface.

摘要翻译： 荧光传感器由以下部分构成：光源，用于发射预定波长的激发光; 介电块，由透射激发光的材料形成; 金属膜，形成在介质块的表面上; 在10〜100nm的范围内，在金属膜上形成疏水性材料的非柔性膜， 样品保持部分，用于保持样品使得样品接触非柔性膜; 入射光学系统，用于使激发光通过介质块进入介电块和金属膜之间的界面，使得满足全内反射的条件; 以及荧光检测装置，用于检测样品内的物质发出的荧光，当激发光进入界面时，由从界面泄漏的ev逝波激发。

公开(公告)号：US07682566B2

公开(公告)日：2010-03-23

申请号：US11333207

申请日：2006-01-18

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N21/00 ,  G01N21/64 ,  G01N30/96 ,  G01N21/55 ,  G01J3/00 ,  C12M1/34

CPC分类号： G01N21/05 ,  G01N21/15 ,  G01N21/553

摘要： A surface plasmon resonance assay apparatus is loaded with a sensor unit. A sensing surface of a thin film detects reaction of a sample. A dielectric prism is overlaid with the thin film to constitute an interface. A reflection angle upon occurrence of attenuated total reflection of the illuminating light is changeable according to reaction of the sample on the sensing surface. Protecting panels are disposed to face outer surfaces of the prism, for covering and protecting at least partially the outer surfaces. A first window in one of the protecting panels is positioned on a path of the illuminating light traveling for incidence on the interface, for passing the illuminating light. A second window in one remaining protecting panel is positioned on a path of the illuminating light traveling upon reflection by the interface, for passing the illuminating light.

摘要翻译： 表面等离子体共振测定装置装载有传感器单元。 薄膜的感测表面检测样品的反应。 介电棱镜与薄膜重叠以构成界面。 发生照射光的全反射衰减时的反射角可以根据检测面上的样品的反应而变化。 保护面板设置成面对棱镜的外表面，用于至少部分地覆盖和保护外表面。 一个保护面板中的第一窗口位于照射光的行进路径上，以便入射在界面上，用于通过照明光。 一个剩余的保护面板中的第二个窗口位于通过界面反射而行进的照明光的路径上，用于通过照明光。

公开(公告)号：US20100047820A1

公开(公告)日：2010-02-25

申请号：US12546147

申请日：2009-08-24

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54373

摘要： A sample is supplied onto the sensor portion of a sensor chip. An excitation light beam is irradiated to generate an enhanced optical field to be generated on the sensor portion. Fluorescent labels are excited, and the amount of a detection target substance is detected, based on the amount of light which is generated due to excitation of the fluorescent labels. A first electric charge is present on the surface of the sensor portion. A fluorescent substance having fluorescent pigment molecules which are enveloped in a light transmitting material that transmits fluorescence generated by the fluorescent pigment molecules, the surfaces of which are charged with second electric charges opposite the first electric charge on the surface are employed as the fluorescent labels. The fluorescent substance is attracted to the sensor portion by static electric interactions between the two charges.

摘要翻译： 将样品供应到传感器芯片的传感器部分。 激发光束被照射以产生将在传感器部分上产生的增强的光场。 荧光标记被激发，并且基于由于荧光标记的激发而产生的光的量，检测目标物质的量。 在传感器部分的表面上存在第一电荷。 具有荧光颜料分子的荧光物质作为荧光标记被使用，荧光颜料分子被包封在透光材料中，该透光材料透射由荧光颜料分子产生的荧光，其表面带有与表面上的第一电荷相反的第二电荷。 荧光物质被两个电荷之间的静电相互作用吸引到传感器部分。

公开(公告)号：US20100009458A1

公开(公告)日：2010-01-14

申请号：US12501923

申请日：2009-07-13

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N21/76

CPC分类号： G01N21/6428 ,  G01N21/05 ,  G01N21/648 ,  G01N21/80 ,  G01N33/54373 ,  G01N2021/0346 ,  Y10S436/805

摘要： A sensor-chip includes a sensor-portion having at least a metal-layer deposited on a surface of a dielectric-plate. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection-target-substance in a liquid-sample binds onto the sensor-portion. The amount of the detection-target-substance is detected based on the amount of light generated by excitation of a fluorescent-label in the fluorescent-label binding-substance. A fluorescent-substance the charge state of which changes in the liquid-sample according to the pH of the liquid-sample, and which contains a plurality of fluorescent-dye-molecules enclosed by a material that transmits fluorescence output from the fluorescent-dye-molecules, is used as the fluorescent-label. With the fluorescent-label binding-substance bound to the sensor-portion, the fluorescent-label is attracted to the sensor-portion by adjusting the pH of the sample-liquid to neutralize the charge state of the fluorescent-substance, thereby attracting the fluorescent-label onto the sensor-portion. In this state, the amount of the detection-target-substance is detected.

摘要翻译： 传感器芯片包括具有至少沉积在电介质板的表面上的金属层的传感器部分。 对应于液体样品中的检测对象物质的量的荧光标记结合物质结合到传感器部分上。 基于荧光标记结合物质中的荧光标记物的激发产生的光量来检测检测对象物质的量。 一种荧光物质，其电荷状态根据液体样品的pH而变化，并且其中含有多个荧光染料分子，所述荧光染料分子由透射荧光染料的荧光输出的材料包围， 分子，被用作荧光标记。 通过与传感器部分结合的荧光标记结合物质，通过调节样品液体的pH以中和荧光物质的电荷状态，将荧光标记物吸引到传感器部分，从而吸引荧光标记物 标记到传感器部分上。 在该状态下，检测出检测对象物质的量。

公开(公告)号：US20090321661A1

公开(公告)日：2009-12-31

申请号：US12492717

申请日：2009-06-26

申请人： Hisashi Ohtsuka

发明人： Hisashi Ohtsuka

IPC分类号： G01N21/64 ,  G01N21/05 ,  G01N21/76

CPC分类号： G01N21/648 ,  G01N21/05 ,  G01N21/7703 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6439

摘要： A fluorescent substance having fluorescent pigment molecules, which are enveloped in a light transmitting material that transmits fluorescence generated by the fluorescent pigment molecules, is employed as fluorescent labels in a detecting method the detects the amount of a detection target substance based on the amount of light which is generated due to excitation of the fluorescent labels. The fluorescent substance is excited by evanescent waves which are generated due to leakage from an optical waveguide mode.

摘要翻译： 作为检测方法中的荧光标记，使用包含在透射由荧光颜料分子产生的荧光的透光性材料中的荧光颜料分子的荧光物质作为荧光标记，基于光量检测检测对象物质的量 这是由于荧光标记的激发而产生的。 荧光物质由于从光波导模式的泄漏而产生的ev逝波被激发。

公开(公告)号：US20090258439A1

公开(公告)日：2009-10-15

申请号：US12422587

申请日：2009-04-13

申请人： Hisashi OHTSUKA

发明人： Hisashi OHTSUKA

IPC分类号： G01N33/558

CPC分类号： G01N33/54386 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808

摘要： An immunochromatoassay method that allows high detection sensitivity measurement. The method including the steps of: permeating an analyte solution that includes a visibly labeled second binding substance that specifically binds to a detection target substance into a test area of a chromatography medium provided with a first binding substance that specifically binds to the detection target substance, simultaneously with or after the permeation of the analyte solution into the test area, permeating a visual recognition aid solution into the chromatograph medium, the solution having a refractive index whose refractive index difference Δn from that of the chromatograph medium is −0.1=Δn=0.1, and visually observing the test area while the visual recognition aid solution is permeated in the test area.

摘要翻译： 允许高检测灵敏度测量的免疫色谱测定方法。 该方法包括以下步骤：将包含特异性结合检测对象物质的可视标记的第二结合物质的分析物溶液渗入具有特异性结合检测对象物质的第一结合物质的色谱介质的检测区域， 在将分析物溶液渗透到测试区域中或之后，将视觉识别助剂溶液渗透到色谱介质中，该折射率与色谱介质的折射率差异Deltan的折射率为-0.1 = Deltan = 0.1 并且在视觉识别辅助溶液渗透到测试区域中的目视观察测试区域。