公开(公告)号：US20110244594A1

公开(公告)日：2011-10-06

申请号：US13045358

申请日：2011-03-10

申请人： Kazuyoshi HORII

发明人： Kazuyoshi HORII

IPC分类号： G01N33/53 ,  C12M1/42

CPC分类号： G01N33/54373

摘要： A target substance detecting method employs a detecting chip equipped with a fine flow channel. Examinations, in which clogging of the flow channel and inhibition of immune reactions can be easily and expediently prevented without preliminary processes, are enabled to be performed without fluctuations in hemolysis rates. The detecting chip having a flow channel base having the flow channel and a detecting portion formed in the flow channel is employed. A liquid sample that may contain a target substance and contains cellular non target substances is caused to flow into the flow channel. An ultrasonic wave emitting section provided upstream of the detecting portion emits ultrasonic waves into the liquid sample from a direction perpendicular to the longitudinal direction of the flow channel such that a standing wave is generated within the flow channel.

摘要翻译： 目标物质检测方法采用具有精细流动通道的检测芯片。 能够在没有溶血速率波动的情况下进行流动通道堵塞和免疫反应抑制的检查，而无需预处理即可方便和方便地进行。 采用具有流路的流路基体和形成在流路中的检测部的检测芯片。 引起可能含有目标物质并含有细胞非目标物质的液体样品流入流路。 设置在检测部上游的超声波发射部从垂直于流路纵向的方向向液体样品发射超声波，使得在流路内产生驻波。

公开(公告)号：US20110236262A1

公开(公告)日：2011-09-29

申请号：US13071142

申请日：2011-03-24

申请人： Kazuyoshi HORII ,  Tomonari Sendai ,  Akihiro Komatsu ,  Mitsuaki Uchida ,  Masashi Hakamata ,  Junpei Shiraishi ,  Daisuke Hibe ,  Yasunori Ohta ,  Akira Ito

发明人： Kazuyoshi HORII ,  Tomonari Sendai ,  Akihiro Komatsu ,  Mitsuaki Uchida ,  Masashi Hakamata ,  Junpei Shiraishi ,  Daisuke Hibe ,  Yasunori Ohta ,  Akira Ito

IPC分类号： B01J19/00

CPC分类号： G01N21/648 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2200/143 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/082

摘要： A biological substance detecting apparatus that enables stable detection and quantitative analysis of biologically derived target substances is provided. The biological substance detecting apparatus employs a sensor chip provided with a fine flow channel through which a sample liquid is caused to flow, and a sensor surface that adsorbs a biologically derived target substance. Light is irradiated onto the sensor surface, and light emitted by the adsorbed target substance or a labeling substance bound thereto is detected. A flow speed controlling means, for controlling the flow speed of the sample liquid that flows through the fine flow channel to a value within a range such that the adsorption speed of the target substance is not controlled by dispersion.

摘要翻译： 提供一种能够对生物衍生的靶物质进行稳定检测和定量分析的生物物质检测装置。 生物物质检测装置采用设置有流过样品液的微细流路的传感器芯片和吸附生物衍生的靶物质的传感器表面。 光照射到传感器表面上，并且检测被吸附的目标物质发出的光或与其结合的标记物质。 一种流量控制装置，用于将流过细流动通道的样品液体的流速控制在使得目标物质的吸附速度不受分散控制的范围内的值。

公开(公告)号：US20090103090A1

公开(公告)日：2009-04-23

申请号：US12255412

申请日：2008-10-21

申请人： Kazuyoshi Horii ,  Toshihito Kimura

发明人： Kazuyoshi Horii ,  Toshihito Kimura

IPC分类号： G01N21/00

CPC分类号： G01N21/7703 ,  G01N21/51 ,  G01N21/552

摘要： The sensing apparatus includes a measuring light emission device, a waveguide member including a sensing surface modified by a surface modification substance, a detection device and an analyzer. The measuring light of a predetermined polarized state is emitted from the emission device so that the measuring light is totally reflected on the sensing surface of the waveguide member holding target substances labeled by fine metal particles to illuminate the particles by evanescent light generated near the sensing surface. The amount of evanescent light scattered by the fine metal particles is detected by the detection device. The measuring light emission device, the waveguide member and the analyzer are included in an optical waveguide system which sets a polarized state of scattered light generated when no target substance is present on the sensing surface in a crossed nicol relation to the analyzer.

摘要翻译： 感测装置包括测量发光装置，包括由表面改性物质改性的感测表面的波导部件，检测装置和分析器。 从发射装置发射预定极化状态的测量光，使得测量光在保持由细金属颗粒标记的目标物质的波导构件的感测表面上全反射，以通过在感测表面附近产生的ev逝光来照射颗粒 。 由金属微粒散射的ev逝光的量由检测装置检测。 测量发光装置，波导构件和分析器被包括在光波导系统中，该光波导系统设定当与分析器以正交尼科关系在感测表面上不存在目标物质时产生的散射光的偏振状态。

公开(公告)号：US08716008B2

公开(公告)日：2014-05-06

申请号：US12906797

申请日：2010-10-18

申请人： Kazuyoshi Horii

发明人： Kazuyoshi Horii

IPC分类号： C12M1/34

CPC分类号： G01N33/54366 ,  G01N33/557

摘要： In a detection method for detecting the quantity of a target material, a labeled binding material in the amount corresponding to the quantity of the target material contained in a liquid specimen is bonded to the top of a sensor portion; and a signal based on light emitted from a label in an evanescent field or an enhanced optical field produced on a surface of the sensor portion when the sensor portion is irradiated with excitation light is detected. After the labeled binding material is bonded to the immobilization layer, the signal is detected while the fluid over the sensor portion is controlled to flow at a constant flow rate at which bonds between the labeled binding material and the immobilization layer are not broken and the above signal can be detected with a greater magnitude than when the liquid specimen exists over the sensor portion at rest.

摘要翻译： 在用于检测目标材料的量的检测方法中，将与液体试样中包含的目标材料的量相对应的量的标记粘合材料粘合到传感器部分的顶部; 并且检测当传感器部分被激发光照射时基于在渐逝场中的标签发射的光或在传感器部分的表面上产生的增强的光场的信号。 标记的粘结材料结合固定层后，检测信号，同时控制传感器部分上的流体以恒定的流速流动，在该流量下，标记的粘结材料和固定层之间的结合不被破坏，上述 可以以比液体样本存在于传感器部分静止时更大的幅度来检测信号。

公开(公告)号：US20120252131A1

公开(公告)日：2012-10-04

申请号：US13432014

申请日：2012-03-28

申请人： Kazuyoshi HORII

发明人： Kazuyoshi HORII

IPC分类号： G01N21/55

CPC分类号： G01N21/8483

摘要： An analyzer includes a holder for a chip having a spreading layer for a sample including a biological material and a reaction layer with a reagent that reacts with the biological material and generates a chromogenic material, a first light source that applies first light that is absorbed by the chromogenic material, a first detector that detects first output light from the chip irradiated with the first light, a second light source that applies second light that is absorbed by the biological material, a second detector that detects second output light from the chip irradiated with the second light, and a calculation unit that calculates a first concentration of the biological material from the first output light, calculates a spreading state of the sample from the second output light, and calculates a second concentration of the biological material by correcting the first concentration using the calculated spreading state.

摘要翻译： 一种分析仪包括：具有用于包含生物材料的样品扩散层的芯片的保持器和具有与生物材料反应并产生显色材料的试剂的反应层;第一光源，其施加由 发色材料，检测来自用第一光照射的芯片的第一输出光的第一检测器，施加被生物材料吸收的第二光的第二光源，第二检测器，其检测来自被照射的芯片的第二输出光 第二光和计算单元，其计算来自第一输出光的生物材料的第一浓度，从第二输出光计算样本的扩展状态，并且通过校正第一浓度来计算生物材料的第二浓度 使用计算出的扩展状态。

公开(公告)号：US20100041065A1

公开(公告)日：2010-02-18

申请号：US12539111

申请日：2009-08-11

申请人： Kazuyoshi Horii ,  Bo Yang

发明人： Kazuyoshi Horii ,  Bo Yang

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N21/648 ,  G01N33/54373 ,  G01N33/6857

摘要： A labeling binding substance in an amount corresponding to the amount of a detection target substance contained in a liquid sample binds to a sensor portion, and the amount of the detection target substance is detected based on the amount of signal light output by excitation of a label of the labeling binding substance in an enhanced optical field on the sensor portion. In this detection method, a labeling substance that includes a light-responsive substance enclosed by a dielectric that transmits light output from the light-responsive substance is used as the label, and the labeling binding substance binds to the sensor portion through a plurality of fragmented antibodies.

摘要翻译： 与液体样品中所含的检测对象物质的量对应的标记结合物质与传感器部分结合，并且基于通过标记的激发输出的信号光的量来检测检测对象物质的量 在传感器部分的增强光场中的标记结合物质。 在该检测方法中，使用包含由透光从光响应物质输出的光的电介质包围的光响应物质的标记物质作为标签，并且标记结合物质通过多个片段结合到传感器部分 抗体。

公开(公告)号：US20100006774A1

公开(公告)日：2010-01-14

申请号：US12501906

申请日：2009-07-13

申请人： Hisashi OHTSUKA ,  Kazuyoshi HORII

发明人： Hisashi OHTSUKA ,  Kazuyoshi HORII

IPC分类号： G01N21/64 ,  G01N21/05

CPC分类号： G01N21/648 ,  G01N2021/6439

摘要： A sensor chip including a sensor portion that has at least a metal layer deposited on a surface of a dielectric plate is used. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection target substance in a liquid sample binds onto the sensor-portion. The amount of the detection target substance is detected based on the amount of light generated by excitation of a fluorescent label in the fluorescent-label binding substance. An electrified fluorescent substance containing a plurality of fluorescent dye molecules enclosed by a material that transmits fluorescence output from the plurality of fluorescent dye molecules is used as the fluorescent label. The electrified fluorescent substance is attracted to the sensor portion by applying voltage to the liquid sample in a state in which the fluorescent-label binding substance has bound to the sensor portion. In this state, the amount of the detection target substance is detected.

摘要翻译： 使用包括至少具有沉积在电介质板的表面上的金属层的传感器部分的传感器芯片。 对应于液体样品中的检测对象物质的量的荧光标记结合物质结合到传感器部分上。 基于荧光标记结合物质中的荧光标记物的激发产生的光量，检测出检测对象物质的量。 作为荧光标记，使用含有多个荧光染料分子的带电荧光物质作为荧光标记。 带电的荧光物质通过在荧光标记结合物质与传感器部分结合的状态下向液体样品施加电压而被吸引到传感器部分。 在该状态下，检测出检测对象物质的量。

公开(公告)号：US20090261269A1

公开(公告)日：2009-10-22

申请号：US12425826

申请日：2009-04-17

申请人： Kazuyoshi HORII ,  Toshihito Kimura

发明人： Kazuyoshi HORII ,  Toshihito Kimura

IPC分类号： G01J1/58 ,  G01N21/64

CPC分类号： G01N21/648 ,  B82Y15/00 ,  B82Y30/00 ,  C09B67/0005 ,  C09B67/0007 ,  C09B67/0013 ,  G01N21/05 ,  G01N21/47 ,  G01N21/6408 ,  G01N21/6428 ,  G01N33/54373 ,  G01N2021/0325 ,  G01N2021/0346 ,  G01N2021/6432

摘要： A sensor chip includes a dielectric plate and a sensor portion having a metal layer deposited on a predetermined area on the dielectric plate. A photo-reactable labeling-substance of an amount corresponding to the amount of a substance to be detected in a sample binds to the sensor portion by contacting the sample with the sensor portion. The amount of the substance to be detected is obtained by irradiating the predetermined area with excitation light and by detecting light output from the photo-reactable labeling-substance in an enhanced electric field that has been generated on the metal layer by irradiation with the excitation light. The photo-reactable labeling-substance includes a photo-reactable substance enclosed by a light transmissive material that transmits light output from the photo-reactable substance to prevent metal quenching that occurs when the photo-reactable substance is located close to the metal layer.

摘要翻译： 传感器芯片包括电介质板和具有沉积在电介质板上的预定区域上的金属层的传感器部分。 与样品中待检测物质的量相对应的光可标记物质通过使样品与传感器部分接触而结合传感器部分。 通过用激发光照射预定区域并且通过用激发光照射在金属层上产生的增强电场中检测光可反应标记物质的光输出来获得待检测物质的量 。 光可反应标记物质包括由透光材料包围的可光反应物质，其透射从光可反应物质输出的光，以防止当光可反应物质靠近金属层时发生的金属淬火。

公开(公告)号：US08637326B2

公开(公告)日：2014-01-28

申请号：US12978148

申请日：2010-12-23

申请人： Kazuyoshi Horii ,  Ryuichi Nakayama ,  Yasutoshi Hirabayashi

发明人： Kazuyoshi Horii ,  Ryuichi Nakayama ,  Yasutoshi Hirabayashi

IPC分类号： G01N33/543

CPC分类号： G01N33/5438 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0809 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/0436

摘要： Detection of detection target substances at a sensor portion is expedited and the efficiency thereof is improved in biological substance analysis, to enable accelerated analysis with high sensitivity. A biological substance analyzing cell equipped with a reaction chamber having a sample supply space, an acoustic matching layer which is provided at a predetermined region of an inner wall of the reaction chamber that faces another inner wall, and a sensor portion provided within the reaction chamber is employed. Ultrasonic waves are emitted such that a standing wave are generated between the acoustic matching layer and the inner wall of the reaction chamber that faces the acoustic matching layer. The detection target substance is concentrated by the capturing forces of the standing waves, and the concentrated detection target substance is detected at the sensor portion.

摘要翻译： 检测传感器部位的检测对象物质，生物物质分析的效率得到提高，能够高灵敏度地加速分析。 一种装备有具有样品供应空间的反应室的生物物质分析池，设置在反应室的内壁相对于另一内壁的预定区域的声匹配层和设置在反应室内的传感器部分 被雇用。 发射超声波，使得在声匹配层和面向声匹配层的反应室的内壁之间产生驻波。 检测对象物质被驻波的捕获力集中，并且在传感器部分检测浓缩检测对象物质。

公开(公告)号：US20110207238A1

公开(公告)日：2011-08-25

申请号：US12978148

申请日：2010-12-23

申请人： Kazuyoshi Horii ,  Ryuichi Nakayama ,  Yasutoshi Hirabayashi

发明人： Kazuyoshi Horii ,  Ryuichi Nakayama ,  Yasutoshi Hirabayashi

IPC分类号： G01N33/543

CPC分类号： G01N33/5438 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0809 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/0436

摘要： Detection of detection target substances at a sensor portion is expedited and the efficiency thereof is improved in biological substance analysis, to enable accelerated analysis with high sensitivity. A biological substance analyzing cell equipped with a reaction chamber having a sample supply space, an acoustic matching layer which is provided at a predetermined region of an inner wall of the reaction chamber that faces another inner wall, and a sensor portion provided within the reaction chamber is employed. Ultrasonic waves are emitted such that a standing wave are generated between the acoustic matching layer and the inner wall of the reaction chamber that faces the acoustic matching layer. The detection target substance is concentrated by the capturing forces of the standing waves, and the concentrated detection target substance is detected at the sensor portion.

摘要翻译： 检测传感器部位的检测对象物质，生物物质分析的效率得到提高，能够高灵敏度地加速分析。 一种装备有具有样品供应空间的反应室的生物物质分析池，设置在反应室的内壁相对于另一内壁的预定区域的声匹配层和设置在反应室内的传感器部分 被雇用。 发射超声波，使得在声匹配层和面向声匹配层的反应室的内壁之间产生驻波。 检测对象物质被驻波的捕获力集中，并且在传感器部分检测浓缩检测对象物质。