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公开(公告)号：US4888695A
公开(公告)日：1989-12-19
申请号：US568876
申请日：1984-01-06
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012
摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph are generated by recording and reproducing a radiation image stored in a stimulable phosphor sheet. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on the support medium and/or synthesized from certain resolved rows. Corresponding reference sampling points in the plural reference rows are joined to generate plural continuous lines comprising straight lines, polygonal lines or curved lines. Sampling points in the remaining non-reference rows are identified by comparison with the continuous lines to thus determine the base sequence of the DNA or DNA fragment.
摘要翻译： 放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分 彼此平行地在支撑介质上形成分辨行。 获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。 通过记录和再现存储在可刺激的磷光体片中的放射线图像来生成与放射自显影仪相对应的电数字信号。 接下来检测数字信号的每个解决行中的采样点。 然后在多个参考行中确定参考采样点，这些参考行直接设置在支持介质上和/或从某些已解决的行合成。 多个参考行中的相应参考采样点被连接以产生包括直线，多边形线或曲线的多个连续线。 通过与连续线进行比较来确定剩余非参考行中的采样点，从而确定DNA或DNA片段的碱基序列。
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公开(公告)号：US4777597A
公开(公告)日：1988-10-11
申请号：US24909
申请日：1987-03-11
CPC分类号： G01T1/2014 , G01T1/2012 , Y10S250/909
摘要： A signal processing method in autoradiography employing a stimulable phosphor sheet for the purpose of obtaining information on the one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of a symbol, numeral or combination thereof, including the steps of:(1) determining a one dimensional scanning line for signal processing; and(2) detecting sampling points on said scanning line,the process being applied to digital signals corresponding to an autoradiograph having the locational information on the radioactively labeled substances, the digital signals being obtained from stimulable phosphor sheet.
摘要翻译： 为了获得关于在支撑介质上以至少一维方向分布的放射性标记的物质的一维位置的信息，以符号，数字或组合的形式，使用可激发的荧光体片的放射自显影的信号处理方法， 包括以下步骤：（1）确定用于信号处理的一维扫描线; 和（2）检测所述扫描线上的采样点，该处理被应用于与具有关于放射性标记物质的位置信息的放射自显影仪对应的数字信号，数字信号是从可刺激的荧光粉片获得的。
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公开(公告)号：US4837733A
公开(公告)日：1989-06-06
申请号：US883922
申请日：1986-07-10
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012
摘要： A signal processing method in autoradiography for obtaining information on the one dimensional location of radioactively labeled substances in a sample where the substances of the sample are distributed in at least one row on a support medium where the information is in the form of symbols, numerals or a combination thereof. The method at least includes the steps of (1) generating digital image data where the data comprises sets of digital signals corresponding to the autoradiograph including the row of distributed substances and where the sets of digital signals may respectively represent successive linear portions of the autoradiograph; (2) scanning selected digital signals of the digital image data to generate at least one further signal representative of a predetermined portion of the row of distributed substances where the position of the predetermined portion is at least partially determined by its location in the scanning direction and the amount of radioactively labeled substance at the location is at least partially determined by the amplitude of the further signal; (3) processing the further signal with either or both of smoothing and threshold-processing to detect sampling point corresponding to the location of the predetermined portion of the row of distributed substances; (4) obtaining the image data digital corresponding to the autoradiograph having the locational information on the radioactively labeled substances where the digital image data is obtained by utilizing a radiation image recording and reproducing method of employing a stimulable phosphor sheet. A signal processing method for determining the base sequence of DNA is also disclosed.
摘要翻译： 一种放射自显影中的信号处理方法，用于获得样品中放射性标记物质的一维位置的信息，其中样品的物质分布在支撑介质上的至少一行中，其中信息为符号，数字或数字的形式 其组合。 该方法至少包括以下步骤：（1）产生数字图像数据，其中数据包括对应于包括分布物质行的放射自显影仪的数字信号组，并且其中数字信号组可以分别表示放射自显影仪的连续线性部分; （2）扫描数字图像数据的所选择的数字信号以产生表示分布物质行的预定部分的至少一个另外的信号，其中预定部分的位置至少部分地由其在扫描方向上的位置确定; 位置处的放射性标记物质的量至少部分地由另外的信号的幅度决定; （3）用平滑和阈值处理中的任一个或两者来处理另外的信号，以检测对应于分配物质行的预定部分的位置的采样点; （4）获得与放射自显影仪对应的图像数据数字，该放射自显影仪具有放射性标记物质的位置信息，其中通过利用使用可刺激荧光粉片的放射线图像记录和再现方法获得数字图像数据。 还公开了用于确定DNA的碱基序列的信号处理方法。
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公开(公告)号：US4852050A
公开(公告)日：1989-07-25
申请号：US568907
申请日：1984-01-06
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012 , Y10S250/909
摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph is then generated by recording and reproducing from a stimulable phosphor sheet the radiation energy stored therein. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on the support medium and/or synthesized from certain resolved rows. Sampling points in the remaining non-reference rows are identified by comparison with the continuous lines to thus determine the base sequence of the DNA or DNA fragment.
摘要翻译： 放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分 彼此平行地在支撑介质上形成分辨行。 获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。 然后通过从可刺激的荧光体片材记录和再现其中存储的辐射能量来生成对应于放射自显影仪的电数字信号。 接下来检测数字信号的每个解决行中的采样点。 然后在多个参考行中确定参考采样点，这些参考行直接设置在支持介质上和/或从某些已解决的行合成。 通过与连续线进行比较来确定剩余非参考行中的采样点，从而确定DNA或DNA片段的碱基序列。
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公开(公告)号：US4665312A
公开(公告)日：1987-05-12
申请号：US773858
申请日：1985-09-09
CPC分类号： G01T1/2942 , G01T1/2014
摘要： A signal detecting method in autoradiography, which is applied to an autoradiography having locational information on radioactively labeled substances such as radioactively labeled biopolymers distributed one-dimensionally, being recorded on a stimulable phosphor sheet as a radiation energy-stored image, which comprises steps of:(1) preliminarily scanning a part of the stimulable phosphor sheet with an electromagnetic wave to release a portion of the radiation energy as stimulated emission and detecting the stimulated emission to give an electric signal, to which a signal processing is applied, to determine one-dimensional distribution direction of the radioactively labeled substances; and(2) finally scanning the stimulable phosphor sheet with an electromagnetic wave along said one-dimensional distribution direction to release the radiation energy as stimulated emission and detecting the stimulated emission, to obtain the locational information on the radioactively labeled substances as a digital signal.
摘要翻译： 一种放射自显影中的信号检测方法，其应用于具有放射性标记物质的位置信息的放射自显影方法，所述放射自显影物质例如放射性标记的一维分布的生物聚合物作为辐射能量存储图像记录在可刺激的磷光体片上，其包括以下步骤： （1）用电磁波对一部分可刺激的荧光体片进行扫描，以释放辐射能量的一部分作为受激发射，并检测受激发射，给出施加信号处理的电信号， 放射性标记物质的尺寸分布方向; 和（2）最后用沿着所述一维分布方向的电磁波扫描所述可刺激的荧光体片材，以释放作为受激发射的辐射能量并检测受激发射，以获得关于放射性标记物质的位置信息作为数字信号。
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公开(公告)号：US4868749A
公开(公告)日：1989-09-19
申请号：US568879
申请日：1984-01-06
申请人： Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29
CPC分类号： G01T1/2012
摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph is then generated. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on resolved row adjacent to the reference row to identify sampling points in adjacent resolved rows. Sampling points in the remaining non-reference rows are identified for determination of the base sequence of the DNA or DNA fragment.
摘要翻译： 放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分 彼此平行地在支撑介质上形成分辨行。 获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。 然后产生对应于放射自显影仪的电数字信号。 接下来检测数字信号的每个解决行中的采样点。 然后在多个参考行中确定参考采样点，这些参考行直接提供在与参考行相邻的解决行上，以标识相邻已解决行中的采样点。 识别其余非参考行中的取样点用于确定DNA或DNA片段的碱基序列。
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公开(公告)号：US4868746A
公开(公告)日：1989-09-19
申请号：US568875
申请日：1984-01-06
申请人： Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01N27/26 , G01N27/447 , G01N33/50 , G01T1/29 , G21H5/02
CPC分类号： G01T1/2012
摘要： A signal processing method in autoradiography employing a radiosensitive material for the purpose of obtaining information on one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof, comprising the steps of obtaining a digital signal corresponding to the autoradiograph on a radiosensitive material, by exposing said radiosensitive material to radiation emitted by said radioactively labeled substances on the support medium determining one dimensional scanning line relative to said digital signal; and detecting sampling points on said scanning line, comprising and identifying the positions of said sampling points detected on said scanning line among resolved rows for determining the base sequence of labeled substances.
摘要翻译： 一种放射自显影的信号处理方法，其采用放射敏感材料，以其符号，数字或组合的形式获得在支撑介质上以至少一维方向分布的放射性标记物质的一维位置的信息，包括步骤 通过将所述放射敏感材料暴露于所述放射性标记的物质在支撑介质上发射的辐射，确定相对于所述数字信号的一维扫描线，获得与放射自显影材料相对应的放射自显影仪的数字信号; 以及检测所述扫描线上的采样点，包括并识别在所述扫描行中检测的所述采样点在已确定的标记物质的基本序列之间的分辨行中的位置。
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公开(公告)号：US4862358A
公开(公告)日：1989-08-29
申请号：US883139
申请日：1986-07-08
申请人： Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29
CPC分类号： G01T1/2012 , Y10S250/909
摘要： A signal processing method in autoradiography for obtaining information on the one dimensional location of radioactively labeled substances in a sample where the substances of the sample are distributed in at least one row on a support medium where the information is in the form of symbols, numerals or a combination thereof. The method at least includes the steps of (1) generating digital image data where the data comprises sets of digital signals corresponding to the autoradiograph including the row of distributed substances and where the sets of digital signals may respectively represent successive linear portions of the autoradiograph; (2) scanning selected digital signals of the digital image data to generate at least one further signal representative of a predetermined portion of the row of distributed substances where the position of the predetermined portion is at least partially determined by its location in the scanning direction and the amount of radioactively labeled substance at the location is at least partially determined by the amplitude of the further signal; (3) processing the further signal with either or both of smoothing and threshold-processing to detect sampling point corresponding to the location of the predetermined portion of the row of distributed substances; (4) obtaining the image data digital corresponding to the autoradiograph having the locational information on the radioactively labeled substances where the digital image data is obtained by utilizing a radiation image recording and reproducing method employing a radiosensitive material. A signal processing method for determining the base sequence of DNA is also disclosed.
摘要翻译： 一种放射自显影中的信号处理方法，用于获得样品中放射性标记物质的一维位置的信息，其中样品的物质分布在支撑介质上的至少一行中，其中信息为符号，数字或数字的形式 其组合。 该方法至少包括以下步骤：（1）产生数字图像数据，其中数据包括对应于包括分布物质行的放射自显影仪的数字信号组，并且其中数字信号组可以分别表示放射自显影仪的连续线性部分; （2）扫描数字图像数据的所选择的数字信号以产生表示分布物质行的预定部分的至少一个另外的信号，其中预定部分的位置至少部分地由其在扫描方向上的位置确定; 位置处的放射性标记物质的量至少部分地由另外的信号的幅度决定; （3）用平滑和阈值处理中的任一个或两者来处理另外的信号，以检测对应于分配物质行的预定部分的位置的采样点; （4）通过利用使用放射敏感材料的放射线图像记录和再现方法，获得与放射自显影仪相对应的具有放射性标记物质的位置信息的图像数据数字，其中获得数字图像数据。 还公开了用于确定DNA的碱基序列的信号处理方法。
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公开(公告)号：US4884200A
公开(公告)日：1989-11-28
申请号：US595504
申请日：1984-03-30
申请人： Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G03B42/02 , G01N27/26 , G01N27/447 , G01N33/50 , G01T1/29
CPC分类号： G01T1/2014 , G01T1/2942
摘要： A signal detecting method in autoradiography, which is applied to an autoradiograph having locational information on radioactively labeled substances such as radioactively labeled biopolymers distributed one-dimensionally, being recorded on a radiosensitive material as a visible image,which comprises steps of:(1) preliminarily scanning a part of the radiosensitive material with a light and photoelectrically reading out a portion of the visualized autoradiograph to give an electric signal, to which a signal processing is applied, to determine one-dimensional distribution direction of the radioactively labeled substances; and(2) finally scanning the radiosensitive material with a light along said one-dimensional distribution direction and photoelectrically reading out the visualized autoradiograph, to obtain the locational information on the radioactively labeled substances as a digital signal.
摘要翻译： 一种放射自显影法中的信号检测方法，其应用于放射自显影仪，其具有放射性标记物质（例如放射性标记的一次分布的生物聚合物）的位置信息，作为可见光图像记录在放射敏感材料上，其包括以下步骤：（1） 通过光和光电读出一部分可视化放射自显影仪来扫描一部分放射敏感材料，以给出施加信号处理的电信号，以确定放射性标记物质的一维分布方向; 和（2）最后用沿着一维分布方向的光扫描放射敏感材料，并光电读出可视化放射自显影仪，获得放射性标记物质的位置信息作为数字信号。
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公开(公告)号：US4871913A
公开(公告)日：1989-10-03
申请号：US161248
申请日：1988-02-19
CPC分类号： G01T1/2942 , H05G1/60
摘要： A signal processing method in autoradiography for determining base sequence of DNA or DNA fragment, employing groups of base-specific synthetic products which are complementary to the DNA or DNA fragment and a labeled with a radioactive element, said groups being resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium,which comprises a process including:(1) determining on each of the resolved rows a scanning line for signal processing;(2) detecting on each of the resolved rows sampling points on said scanning line; and(3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, ademine, thymine and cytosine;said process being applied to digital signals corresponding to an autoradiograph having the locational information on the radioactively labeled synthetic products, said digital signals being obtained by the use of a stimulable phosphor sheet or by the use of a radiosensitive material.
摘要翻译： 用于确定DNA或DNA片段的碱基序列的放射自显影术中的信号处理方法，使用与DNA或DNA片段互补的碱基特异性合成产物和用放射性元素标记的基团，所述基团平行一维地解析 相互关系以在支撑介质上形成分辨行，其包括以下处理：（1）在每个解决的行上确定用于信号处理的扫描线; （2）对所述扫描线上的每个解析行采样点进行检测; 和（3）比较和识别所解决的行中扫描线上的所述采样点的位置，以获得关于鸟嘌呤，腺嘌呤，胸腺嘧啶和胞嘧啶的位置信息; 所述方法应用于与放射自显影仪对应的数字信号，该放射自显影仪具有关于放射性标记的合成产品的位置信息，所述数字信号通过使用可刺激的荧光粉片或通过使用放射敏感材料获得。
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