公开(公告)号：US20210284959A1

公开(公告)日：2021-09-16

申请号：US17162207

申请日：2021-01-29

Applicant: Hitachi, Ltd.

Inventor： Yuki NIIMI ,  Hiroko HANZAWA ,  Maiko TANABE ,  Kunio OHYAMA ,  Shizu TAKEDA

IPC: C12N5/073 ,  C12M1/36

Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.

公开(公告)号：US20230407228A1

公开(公告)日：2023-12-21

申请号：US18108527

申请日：2023-02-10

Applicant: Hitachi, Ltd.

Inventor： Takayuki NOZAKI ,  Yuki NIIMI ,  Masaharu KIYAMA ,  Kakuro HIRAI

IPC: C12M1/36 ,  C12M1/32 ,  C12M1/34 ,  C12Q1/02

CPC classification number: C12M41/48 ,  C12M23/12 ,  C12M41/46 ,  C12Q1/02

Abstract: A cell sheet cutting assistance system comprises a processor for executing a program and a storage device for storing the program. The processor: receives, as input, an evaluation standard of a cell sheet and quality information of a cultured cell sheet; determines whether the quality information satisfies the evaluation standard; and outputs a cutting position of the cell sheet based on a result of the determination.