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公开(公告)号：US08309516B2
公开(公告)日：2012-11-13
申请号：US12529246
申请日：2008-03-26
申请人： Hope Hart , Jeng S. Chen , Cheryl Stacy , Frederick Walters
发明人： Hope Hart , Jeng S. Chen , Cheryl Stacy , Frederick Walters
IPC分类号： C07K14/325
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins (eHIPs) having toxicity to at least corn rootworm are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different Bacillus thuringiensis (Bt) Cry proteins or a modified Cry proteins an eHIP having activity against corn rootworm is designed. Nucleic acid molecules encoding the novel eHIPs are also provided. Methods of making the eHIPs and methods of using the eHIPs and nucleic acids encoding the eHIPs of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.
摘要翻译： 公开了用于控制植物害虫的组合物和方法。 特别地，提供了对至少玉米根虫具有毒性的新型工程化杂合杀虫蛋白（eHIP）。 通过融合至少两种不同的苏云金芽孢杆菌（Bt）Cry蛋白或修饰的Cry蛋白的完全或部分可变区和保守嵌段的独特组合，设计具有抗玉米根虫活性的eHIP。 还提供了编码新型eHIP的核酸分子。 还公开了制备eHIP的方法和使用编码本发明的eHIP的eHIP和核酸的方法，例如在转基因植物中赋予防御昆虫损伤的方法。
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公开(公告)号：US20100017914A1
公开(公告)日：2010-01-21
申请号：US12529246
申请日：2008-03-26
申请人： Hope Hart , Jeng S. Chen , Cheryl Stacy , Frederick Walters
发明人： Hope Hart , Jeng S. Chen , Cheryl Stacy , Frederick Walters
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins (eHIPs) having toxicity to at least corn rootworm are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different Bacillus thuringiensis (Bt) Cry proteins or a modified Cry proteins an eHIP having activity against corn rootworm is designed. Nucleic acid molecules encoding the novel eHIPs are also provided. Methods of making the eHIPs and methods of using the eHIPs and nucleic acids encoding the eHIPs of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.
摘要翻译： 公开了用于控制植物害虫的组合物和方法。 特别地，提供了对至少玉米根虫具有毒性的新型工程化杂合杀虫蛋白（eHIP）。 通过融合至少两种不同的苏云金芽孢杆菌（Bt）Cry蛋白或修饰的Cry蛋白的完全或部分可变区和保守嵌段的独特组合，设计具有抗玉米根虫活性的eHIP。 还提供了编码新型eHIP的核酸分子。 还公开了制备eHIP的方法和使用编码本发明的eHIP的eHIP和核酸的方法，例如在转基因植物中赋予防御昆虫损伤的方法。
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公开(公告)号：US20080182796A1
公开(公告)日：2008-07-31
申请号：US11799009
申请日：2007-04-30
申请人： Jeng S. Chen , Cheryl Stacy , Frederick Walters
发明人： Jeng S. Chen , Cheryl Stacy , Frederick Walters
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling plant pests are disclosed. In particular, novel nucleic acid sequences encoding modified Cry3A toxins having increased toxicity to corn rootworm are provided. By inserting a protease recognition site that is recognized by a gut protease of a target insect in at least one position of a Cry3A toxin a modified Cry3A toxin having significantly greater toxicity, particularly to western and northern corn rootworm is designed. Further, a method of making the modified Cry3A toxins and methods of using the modified cry3A nucleic acid sequences, for example in microorganisms to control insects or in transgenic plants to confer protection from insect damage, and a method of using the modified Cry3A toxins, and compositions and formulations comprising the modified Cry3A toxins, for example applying the modified Cry3A toxins or compositions or formulations to insect-infested areas, or to prophylactically treat insect-susceptible areas or plants to confer protection against the insect pests are disclosed.
摘要翻译： 公开了用于控制植物害虫的组合物和方法。 特别地，提供了对玉米根虫具有增加的毒性的编码修饰的Cry3A毒素的新型核酸序列。 通过将目标昆虫的肠道蛋白酶识别的蛋白酶识别位点插入Cry3A毒素的至少一个位置，具有显着更大毒性的改良的Cry3A毒素，特别是对西方和北方的玉米根虫进行设计。 此外，制备修饰的Cry3A毒素的方法和使用经修饰的cry3A核酸序列的方法，例如在微生物中控制昆虫或在转基因植物中赋予防御昆虫损伤的方法，以及使用经修饰的Cry3A毒素的方法，以及 公开了包含修饰的Cry3A毒素的组合物和制剂，例如将经修饰的Cry3A毒素或组合物或制剂施用于昆虫侵袭区域，或用于预防性治疗昆虫敏感区域或植物以赋予对昆虫害虫的保护。
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