公开(公告)号：US20230235315A1

公开(公告)日：2023-07-27

申请号：US18011311

申请日：2021-07-09

申请人： Horizon Discovery Limited

发明人： John Lambourne ,  Immacolata Porreca

IPC分类号： C12N15/10 ,  C12N5/0783 ,  C12N9/24

CPC分类号： C12N15/102 ,  C12N5/0636 ,  C12Y302/02027 ,  C12N9/2497 ,  C12Y305/04004 ,  C07K2319/00 ,  C12N2510/00

摘要： A method for producing genetically engineered immune cells, e.g. T cells, or iPSCs which uses an RNA-scaffold mediated base editing system. The method enables precise modifications to be made to the genome whilst minimizing the possibility of off-target effects, making the method particularly suitable for therapeutic applications.

公开(公告)号：US20240067954A1

公开(公告)日：2024-02-29

申请号：US18270879

申请日：2022-01-05

申请人： Horizon Discovery Limited

发明人： John Lambourne ,  Immacolata Porreca ,  Amanda Smith

IPC分类号： C12N15/10 ,  C12N9/22

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N2310/20

摘要： The present invention relates to an RNA-mediated base editing system that enables recruitment of multiple effector proteins to a single target site, allowing for highly efficient base editing. The invention further relates to methods and kits for genetically modifying a cell.