公开(公告)号：US20180163254A1

公开(公告)日：2018-06-14

申请号：US15839597

申请日：2017-12-12

申请人： SYNTHETIC GENOMICS, INC.

发明人： JOHN E. GILL ,  Daniel G. Gibson ,  Lixia Fu

IPC分类号： C12Q1/686 ,  C07H19/173 ,  C07H19/073 ,  C12N9/22

CPC分类号： C12Q1/686 ,  C07H19/073 ,  C07H19/173 ,  C12N9/22 ,  C12N15/09 ,  C12N15/10 ,  C12N15/1031 ,  C12P19/34 ,  C12Y302/02027 ,  C12Q2521/101 ,  C12Q2533/101

摘要： The invention provides compositions and methods for assembling a DNA molecule having a desired sequence. The methods involve contacting a DNA polymerase, dNTPs, and a plurality of pairs of oligonucleotides. The oligonucleotides of a pair have a portion of the desired sequence, and an internal sequence that overlaps and is complementary to an internal sequence of the other oligonucleotide of the pair, and, when arranged in order, they have at least a portion of the desired sequence. The oligonucleotides also have a 3′ or a 5′ primer binding sequence having a binding site for a primer. The oligonucleotides that correspond to the end oligonucleotides of the desired sequence also have a universal 3′ flanking sequence and a universal 5′ flanking sequence, respectively. The methods involve performing a first amplification reaction on the plurality of pairs of oligonucleotides; removing the 3′ and 5′ primer binding sequences from the plurality of pairs of oligonucleotides; and subjecting the plurality of pairs of oligonucleotides to an assembly reaction to thereby assemble the dsDNA molecule having the desired sequence.

公开(公告)号：US20170137807A1

公开(公告)日：2017-05-18

申请号：US15317227

申请日：2015-06-11

申请人： Vela Operations Pte. Ltd.

发明人： Rui ZHANG ,  Leong Ting SEE ,  Siow San ROUGH ,  Yeo Qiang YONG ,  Arseny SMIRNOV ,  Lou Ping CHAO

IPC分类号： C12N15/10 ,  C12Q1/70 ,  C12N9/24 ,  C12Q1/68

CPC分类号： C12N15/1096 ,  C12N9/2497 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/706 ,  C12Y207/00 ,  C12Y207/07049 ,  C12Y302/02027 ,  C12Q2521/531 ,  C12Q2535/122

摘要： The present invention relates to improved semi-automated methods that permit the extraction of nucleic acids from samples, preparation of PCR and post-PCR preparation steps of DNA- libraries for next-generation sequencings methods that can be conducted. The methods and additional aspects relating to such methods are less laborious, safe costs, reagents and are less prone to contamination than comparable methods that are not automated.

公开(公告)号：US20230235315A1

公开(公告)日：2023-07-27

申请号：US18011311

申请日：2021-07-09

申请人： Horizon Discovery Limited

发明人： John Lambourne ,  Immacolata Porreca

IPC分类号： C12N15/10 ,  C12N5/0783 ,  C12N9/24

CPC分类号： C12N15/102 ,  C12N5/0636 ,  C12Y302/02027 ,  C12N9/2497 ,  C12Y305/04004 ,  C07K2319/00 ,  C12N2510/00

摘要： A method for producing genetically engineered immune cells, e.g. T cells, or iPSCs which uses an RNA-scaffold mediated base editing system. The method enables precise modifications to be made to the genome whilst minimizing the possibility of off-target effects, making the method particularly suitable for therapeutic applications.

公开(公告)号：US20180170984A1

公开(公告)日：2018-06-21

申请号：US15836598

申请日：2017-12-08

申请人： Regents of the University of Minnesota

发明人： Reuben S. Harris ,  Hideki Aihara

IPC分类号： C07K14/47 ,  C12N9/22 ,  C12N15/10

CPC分类号： C07K14/4703 ,  C07K2319/80 ,  C07K2319/85 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N2310/20 ,  C12Y302/02027 ,  C12Y305/04005

摘要： Materials and methods for using modified Cas9-APOBEC fusion polypeptides for targeted modification of specific DNA sequences are provided herein.

公开(公告)号：US20060134631A1

公开(公告)日：2006-06-22

申请号：US10746239

申请日：2003-12-23

申请人： Hans Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John Tainer ,  Clifford Mol

发明人： Hans Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John Tainer ,  Clifford Mol

IPC分类号： C12Q1/68 ,  C12N9/22

CPC分类号： C12Q1/6848 ,  C12N9/2497 ,  C12Q1/6869 ,  C12Y302/02027 ,  C12Q2521/531

摘要： Novel cytosine-, thymine- and uracil-DNA glycosylases, subcellular localization peptides, nucleic acid molecules containing the same, methods of identifying such enzymes and their use in various methods including mutagenesis, cell killing and DNA sequencing and modification, are desired.

摘要翻译： 新的胞嘧啶 - ，胸腺嘧啶核苷和尿嘧啶-DNA糖基化酶，亚细胞定位肽，含有这些肽的核酸分子，鉴定这些酶的方法及其在各种方法中的应用，包括诱变，细胞杀伤和DNA测序和修饰。

公开(公告)号：US20230193234A1

公开(公告)日：2023-06-22

申请号：US18051933

申请日：2022-11-02

申请人： Xiamen University ,  Xiamen Zeesan Biotech Co., Ltd.

发明人： Yongyou ZHANG ,  Dan WANG ,  Najie SONG

IPC分类号： C12N9/24 ,  C12N15/70

CPC分类号： C12N9/2497 ,  C12Y302/02027 ,  C12N15/70 ,  C12N2800/101 ,  C07K2319/21 ,  C07K2319/95

摘要： A recombinant vector of a thermolabile UNG fused protein and an expressing and purifying method are provided. The method comprises cloning a Cod UNG genetic sequence to a pCold-SUMO vector to construct a recombinant vector pCold-SUMO-Cod UNG, transforming to E. coli BL21 (DE3) competent cells, transforming and expressing molecular chaperone plasmids pG-Tf2, and inducing the expression at a low temperature to obtain a soluble SUMO-Cod UNG fused protein.

公开(公告)号：US20170321210A1

公开(公告)日：2017-11-09

申请号：US15523939

申请日：2015-11-02

申请人： National University Corporation Kobe University

发明人： Keiji NISHIDA ,  Akihiko KONDO

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N9/24 ,  C12N2310/20

CPC分类号： C12N15/1024 ,  C07K19/00 ,  C12N9/22 ,  C12N9/24 ,  C12N9/2497 ,  C12N15/09 ,  C12N15/11 ,  C12N2310/20 ,  C12Y302/02027

摘要： The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

公开(公告)号：US07662601B2

公开(公告)日：2010-02-16

申请号：US10746239

申请日：2003-12-23

申请人： Hans E. Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John A. Tainer ,  Clifford D. Mol

发明人： Hans E. Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John A. Tainer ,  Clifford D. Mol

IPC分类号： C12N9/10 ,  C12N15/00 ,  C12P21/04 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/48 ,  C07H21/04 ,  C12Q1/00 ,  C12N1/20 ,  C07H21/02

CPC分类号： C12Q1/6848 ,  C12N9/2497 ,  C12Q1/6869 ,  C12Y302/02027 ,  C12Q2521/531

摘要： Novel cytosine-, thymine- and uracil-DNA glycosylases, subcellular localization peptides, nucleic acid molecules containing the same, methods of identifying such enzymes and their use in various methods including mutagenesis, cell killing and DNA sequencing and modification, are desired.

摘要翻译： 新型胞嘧啶 - ，胸腺嘧啶核苷和尿嘧啶-DNA糖基化酶，亚细胞定位肽，含有该核酸分子的核酸分子，鉴定此类酶的方法及其在各种方法中的应用，包括诱变，细胞杀伤和DNA测序和修饰。

公开(公告)号：US06713294B1

公开(公告)日：2004-03-30

申请号：US09101368

申请日：1999-03-03

申请人： Hans E. Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John A. Tainer ,  Clifford D. Mol

发明人： Hans E. Krokan ,  Geir Slupphaug ,  Bodil Kavli ,  John A. Tainer ,  Clifford D. Mol

IPC分类号： C12N914

CPC分类号： C12Q1/6848 ,  C12N9/2497 ,  C12Q1/6869 ,  C12Y302/02027 ,  C12Q2521/531

摘要： Novel cytosine-, thymine- and uracil-DNA glycosylases, subcellular localization peptides, nucleic acid molecules containing the same, methods of identifying such enzymes and their use in various methods including mutagenesis, cell killing and DNA sequencing and modification, are described.

摘要翻译： 描述了新型胞嘧啶 - ，胸腺嘧啶核苷和尿嘧啶-DNA糖基化酶，亚细胞定位肽，含有这些肽的核酸分子，鉴定这些酶的方法及其在各种方法中的应用，包括诱变，细胞杀伤和DNA测序和修饰。

公开(公告)号：US20230257789A1

公开(公告)日：2023-08-17

申请号：US17670372

申请日：2022-02-11

申请人： MICROSOFT TECHNOLOGY LICENSING, LLC

发明人： Yuan-Jyue CHEN ,  Bichlien Hoang NGUYEN ,  Karin STRAUSS

IPC分类号： C12P19/34 ,  C12N15/11 ,  C12N9/00 ,  C12N9/24 ,  C12N9/22 ,  B01L3/00 ,  C07H21/00

CPC分类号： C12P19/34 ,  B01L3/502715 ,  B01L3/502761 ,  C07H21/00 ,  C12N9/22 ,  C12N9/93 ,  C12N9/2497 ,  C12N15/11 ,  C12Y301/26004 ,  C12Y302/02027 ,  C12N2310/531

摘要： Sequential assembly of oligonucleotide hairpins is used to create oligonucleotides with specific sequences. Each oligonucleotide hairpin includes a payload region containing one or more nucleotides that are added to the end of an anchor strand. Overhang regions on the oligonucleotide hairpins hybridize to anchor strands attached to a substrate. The hybridized oligonucleotide hairpins are covalently attached to the anchor strands by the activity of ligase. The oligonucleotide hairpins include an enzyme cleavage region which, when cleaved, separates the payload region from the remainder of the oligonucleotide hairpin. The oligonucleotide hairpin is separated from the anchor strand by denaturation and washed away. This process is repeated with additional oligonucleotide hairpins to add additional nucleotides to the ends of the anchor strands. A microelectrode array may be used to control the location of hybridization and create multiple oligonucleotides in parallel. Fully assembled oligonucleotides can be separated from the substrate and stored.