Inhibition of hepatitis B virus (HBV) replication by RNA interference
    6.
    发明授权
    Inhibition of hepatitis B virus (HBV) replication by RNA interference 有权
    通过RNA干扰抑制乙型肝炎病毒(HBV)复制

    公开(公告)号:US07067249B2

    公开(公告)日:2006-06-27

    申请号:US10848736

    申请日:2004-05-19

    IPC分类号: C07H21/04

    摘要: The present invention relates to the inhibition of Hepatitis B virus (HBV) replication by RNA molecules of the present invention. Specifically, the RNA molecules of the present invention are double-stranded ribonucleic acid molecules (dsRNA). Specifically, the invention relates to small interfering RNAs (siRNA) which are double-stranded RNAs that direct the sequence-specific degradation of messenger RNA in mammalian cells. The invention relates to development of a new anti-HBV therapy by inhibition of Hepatitis B Virus (HBV) replication using stably-expressed short hairpin RNAs (shRNA), which degrade HBV pregenomic RNA and message RNAs. Included are methods of treatment of cancer by the administration of RNA molecules of the present invention in combination with surgery, alone or in further combination with standard and experimental chemotherapies, hormonal therapies, biological therapies/immunotherapies and/or radiation therapies.

    摘要翻译: 本发明涉及通过本发明的RNA分子对乙型肝炎病毒(HBV)复制的抑制。 具体地说,本发明的RNA分子是双链核糖核酸分子(dsRNA)。 具体地,本发明涉及作为引导哺乳动物细胞中信使RNA的序列特异性降解的双链RNA的小干扰RNA(siRNA)。 本发明涉及通过使用稳定表达的短发夹RNA(shRNA)抑制乙型肝炎病毒(HBV)复制来降低HBV前基因组RNA和消息RNA来开发新的抗HBV治疗。 包括通过单独或与标准和实验化学疗法,激素治疗,生物疗法/免疫疗法和/或放射疗法单独或进一步组合的本发明的RNA分子的给药方法来治疗癌症的方法。