公开(公告)号：US20230272350A1

公开(公告)日：2023-08-31

申请号：US18016150

申请日：2021-07-12

申请人： Hvidovre Hospital ,  Københavns Universitet ,  Technische Hochschule Mittelhessen Institute für Bioverfahrenstechnik und Pharmazeutische Technologi

发明人： Judith Margarete Gottwein ,  Jens Bukh ,  Anna Offersgaard ,  Anne Finne Pihl ,  Michael Wolff ,  Keven Lothert

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N2770/24252 ,  C12N2770/24222 ,  C12N2770/24223 ,  C12N2770/24234 ,  C12N2770/24261

摘要： The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and/or concentration of the cell culture supernatant, c) purification and/or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and/or concentration of the product of above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining purified whole virus particles.