公开(公告)号：US20220259603A1

公开(公告)日：2022-08-18

申请号：US17610278

申请日：2020-05-21

申请人： Hyasynth Biologicals Inc.

发明人： Leanne BOURGEOIS ,  Alexander CAMPBELL ,  Elizabeth-Ann KRANJEC ,  Mindy MELGAR ,  Shoham MOOKERJEE ,  Sylvester PALYS ,  Alexandre THERRIEN ,  Curtis WALTON ,  Kevin WOO ,  Xiaohua ZHANG

IPC分类号： C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12N9/10 ,  C12P17/04 ,  C12P7/22 ,  C12P7/42

摘要： The present disclosure relates generally to methods and cell lines for the production of phytocannabinoids, phytocannabinoid precursors or intermediates, or phytocannabinoid analogue. Methods for transformation of host cells, such as yeast cells, are described. Cells may be transformed, for example, with a polynucleotide encoding a polyketide synthase (PKS) enzyme, a polynucleotide encoding an olivetolic acid cyclase (OAC) enzyme, and/or a polynucleotide encoding a prenyltransferase (PT) enzyme; and optionally a polynucleotide encoding an acyl-CoA synthase (Alk) enzyme; a polynucleotide encoding a fatty acyl CoA activating (CsAAE) enzyme; and/or a polynucleotide encoding a THCa synthase (OXC) enzyme.