公开(公告)号：US20220259603A1

公开(公告)日：2022-08-18

申请号：US17610278

申请日：2020-05-21

申请人： Hyasynth Biologicals Inc.

发明人： Leanne BOURGEOIS ,  Alexander CAMPBELL ,  Elizabeth-Ann KRANJEC ,  Mindy MELGAR ,  Shoham MOOKERJEE ,  Sylvester PALYS ,  Alexandre THERRIEN ,  Curtis WALTON ,  Kevin WOO ,  Xiaohua ZHANG

IPC分类号： C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12N9/10 ,  C12P17/04 ,  C12P7/22 ,  C12P7/42

摘要： The present disclosure relates generally to methods and cell lines for the production of phytocannabinoids, phytocannabinoid precursors or intermediates, or phytocannabinoid analogue. Methods for transformation of host cells, such as yeast cells, are described. Cells may be transformed, for example, with a polynucleotide encoding a polyketide synthase (PKS) enzyme, a polynucleotide encoding an olivetolic acid cyclase (OAC) enzyme, and/or a polynucleotide encoding a prenyltransferase (PT) enzyme; and optionally a polynucleotide encoding an acyl-CoA synthase (Alk) enzyme; a polynucleotide encoding a fatty acyl CoA activating (CsAAE) enzyme; and/or a polynucleotide encoding a THCa synthase (OXC) enzyme.

公开(公告)号：US20220290194A1

公开(公告)日：2022-09-15

申请号：US17828449

申请日：2022-05-31

申请人： Hyasynth Biologicals Inc.

发明人： Letian SONG ,  Timothy S. LIAO ,  Curtis WALTON ,  Louis HOM ,  Mindy MELGAR ,  Daniel FURLONG ,  Devanshi BHARGAVA ,  Sylvester PALYS ,  Leanne BOURGEOIS

IPC分类号： C12P7/42 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

摘要： The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of cannabidiolic acid (CBDa), cannabigerolic acid (CBCa), and other phytocannabinoids. A method of producing CBDa, CBCa, and/or other phytocannabinoids in a heterologous host cell having CBDa-producing, CBCa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant CBDa synthase protein having a serine insertion between residues P224 and K225 and one or more other amino acid mutation relative to wild type CBDa synthase, and culturing the transformed host cell to produce CBDa, CBCa, and/or other phytocannabinoids therefrom. The variant CBDa synthase protein has at least 85% sequence identity with the wild type CBDa synthase protein sequence OXC52 according to SEQ ID NO:140, with serine insertion (SEQ ID NO:141). Exemplary variants having good phytocannabinoid production capacity are described.