公开(公告)号：US20240109063A1

公开(公告)日：2024-04-04

申请号：US18374062

申请日：2023-09-28

Applicant: ILLUMINA, INC.

Inventor： Dustin BLAIR ,  Patrick WEN ,  John EARNEY ,  Anmiv PRABHU ,  Rachel ABASKHARON ,  Gregory HOLST ,  Chia-Hsi LIU ,  Ravi THAKUR ,  Dakota WATSON ,  Kevin BARTIG ,  Daeyong SIM

IPC: B01L3/00 ,  C12Q1/6874 ,  G01N21/64

CPC classification number: B01L3/502715 ,  C12Q1/6874 ,  G01N21/6428 ,  B01L2200/148 ,  B01L2300/0654 ,  G01N2021/6439 ,  G01N2201/12723

Abstract: An apparatus includes a flow cell, an imaging assembly, and a processor. The flow cell includes a channel and a plurality of reaction sites. The imaging assembly is operable to receive light emitted from the reaction sites in response to an excitation light. The processor is configured to drive relative movement between at least a portion of the imaging assembly and the flow cell along a continuous range of motion to thereby enable the imaging assembly to capture images along the length of the channel. The processor is also configured to activate the imaging assembly to capture one or more calibration images of one or more calibration regions of the channel, during a first portion of the continuous range of motion. The processor is also configured to activate the imaging assembly to capture images of the reaction sites during a second portion of the continuous range of motion.