公开(公告)号：US20210087613A1

公开(公告)日：2021-03-25

申请号：US17026069

申请日：2020-09-18

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Fiona E. Black ,  Jeffrey Dennis Brodin ,  Lorenzo Berti ,  Siew Hong Leong ,  Jeffrey S. Fisher ,  Allen Eckhardt ,  Rui Zhang ,  Yin Nah Teo

IPC: C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. Some embodiments relate to sequencing target polynucleotides from several different nucleic acids samples on a bead array.