公开(公告)号：US20230357307A1

公开(公告)日：2023-11-09

申请号：US18311876

申请日：2023-05-03

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE LTD.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Min Yen Lee ,  Ramesh Neelakandan ,  Jeffrey G. Mandell ,  Erin Garcia ,  Sharyuen Soh ,  Daniel Hartoyo Lukamto

IPC: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

CPC classification number: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.

公开(公告)号：US20250109433A1

公开(公告)日：2025-04-03

申请号：US18887792

申请日：2024-09-17

Applicant: Illumina, Inc.

Inventor： Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Ramesh Neelakandan ,  Erin Garcia ,  Abdul Sadeer Abd Salam ,  Daniel Hartoyo Lukamto ,  Soumadwip Ghosh ,  Xiangyuan Yang ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6853

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a macromolecular block. In some embodiments, the macromolecular blocks are configured for slowing or halting the polynucleotide translocation through a nanopore. In some embodiments the macromolecular blocks have linear and branched structures.

公开(公告)号：US20240191292A1

公开(公告)日：2024-06-13

申请号：US18523636

申请日：2023-11-29

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Hassan Bohra ,  Hui Kheng Karen Goh ,  Yin Nah Teo ,  Wei Wei

IPC: C12Q1/6874 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0861

Abstract: An example flow cell includes a substrate including depressions separated by interstitial regions; a polymeric hydrogel positioned within each of the depressions; a primer set attached to the polymeric hydrogel; and one of: a passivation component attached to the interstitial regions, or a passivation component attached to the polymeric hydrogel, or respective passivation components attached to each of the interstitial regions and the polymeric hydrogel.

公开(公告)号：US20210381040A1

公开(公告)日：2021-12-09

申请号：US17396532

申请日：2021-08-06

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Yin Nah Teo ,  Xi-Jun Chen ,  Tarun Kumar Khurana

IPC: C12Q1/6855 ,  C12Q1/6806

Abstract: Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.

公开(公告)号：US20250002974A1

公开(公告)日：2025-01-02

申请号：US18758066

申请日：2024-06-28

Applicant: Illumina, Inc.

Inventor： Sherman Lauw ,  Trina Osothprarop ,  Henry Day ,  Johan Basuki ,  Claire Bevis-Mott ,  Hester Beard ,  Chol Steven Yun ,  Faye Huber ,  Patrizia Iavicoli ,  Yin Nah Teo ,  Mei Xian Agnes Lee ,  Denise Ng

IPC: C12Q1/6806 ,  C12Q1/6874

Abstract: This application relates to methods and compositions for protecting fully functional nucleotides (ffNs) against degradation. In some examples, micelles or surfactants are used to protect ffNs. In some examples, bulky cation compounds are used to protect ffNs. In some examples, the compositions that protect ffNs are included in lyophilized material.

公开(公告)号：US20210087613A1

公开(公告)日：2021-03-25

申请号：US17026069

申请日：2020-09-18

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Fiona E. Black ,  Jeffrey Dennis Brodin ,  Lorenzo Berti ,  Siew Hong Leong ,  Jeffrey S. Fisher ,  Allen Eckhardt ,  Rui Zhang ,  Yin Nah Teo

IPC: C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. Some embodiments relate to sequencing target polynucleotides from several different nucleic acids samples on a bead array.

公开(公告)号：US10851131B2

公开(公告)日：2020-12-01

申请号：US16627035

申请日：2019-02-19

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:

公开(公告)号：US20250019758A1

公开(公告)日：2025-01-16

申请号：US18650800

申请日：2024-04-30

Applicant: ILLUMINA, INC.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Tsz Ying Yuen ,  Daniel Hartoyo Lukamto ,  Erin Garcia ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods for modification-based controlled polynucleotide translocation in nanopores for sequencing, modified nucleotides, and kits and systems for performing the disclosed methods. In some embodiments, modifications can be used to control polynucleotide translocation by modifying nucleotides on a strand of polynucleotide to carry a modification, where the modifications can arrest or slow translocation when encountering the nanopore. In some embodiments, application of a voltage can move one nucleotide and its attached modification through the nanopore at a time.

公开(公告)号：US20240317793A1

公开(公告)日：2024-09-26

申请号：US18608556

申请日：2024-03-18

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Yin Nah Teo ,  Abdul Sadeer Abd Salam ,  Eric Murtfeldt

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: In some examples, a nucleoside triphosphate analogue may include a sugar, a nucleobase coupled to the sugar, a triphosphate group coupled to the sugar, a heteroatom coupled to an alpha phosphate of the triphosphate group, and a first substituent coupled to the heteroatom. The heteroatom may be selected from the group consisting of oxygen, nitrogen, and carbon. The first substituent may include at least one of an alkyl chain or a polymer.

公开(公告)号：US11578094B2

公开(公告)日：2023-02-14

申请号：US17106963

申请日：2020-11-30

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I: